A5100706977- Metabolomics and Mass Spectrometry Studies
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Nanoplatforms for cancer theranostics
- Cancer Risks and Factors
- MicroRNA in disease regulation
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Phagocytosis and Immune Regulation
- Cancer, Hypoxia, and Metabolism
- Photodynamic Therapy Research Studies
- RNA Interference and Gene Delivery
- Lung Cancer Diagnosis and Treatment
- Cancer Immunotherapy and Biomarkers
- interferon and immune responses
- Breast Cancer Treatment Studies
- Cancer, Lipids, and Metabolism
- Lung Cancer Treatments and Mutations
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Circular RNAs in diseases
- Esophageal Cancer Research and Treatment
- Antibiotics Pharmacokinetics and Efficacy
- Parkinson's Disease Mechanisms and Treatments
- Lung Cancer Research Studies
Institute of Zoology
2023-2025
Guangdong Academy of Sciences
2023-2025
University of Science and Technology of China
2024-2025
Tianjin Medical University Cancer Institute and Hospital
2021-2025
Ministry of Agriculture and Rural Affairs
2021-2025
Nanjing Agricultural University
2015-2025
Henan University
2024-2025
First Affiliated Hospital of Zhengzhou University
2020-2025
Second Affiliated Hospital of Zhejiang University
2018-2025
Zhejiang University
2021-2025
Background & AimsWe aimed to identify long noncoding RNAs (lncRNAs) that are up-regulated in gastric cancer tissues from patients and study their function tumor metastasis.MethodsWe collected nontumor China analyzed levels of lncRNAs by microarray analysis, proteins immunohistochemistry, quantitative reverse-transcription polymerase chain reaction; we compared these with survival times progression. RNA were knocked down or out BGC-823, SGC-7901, MKN45 cell lines using small interfering short...
Microbiota-host interactions play critical roles in colorectal cancer (CRC) progression, however, the underlying mechanisms remain elusive. Here, we uncover that Fusobacterium nucleatum (F. nucleatum) induces a dramatic decline of m6A modifications CRC cells and patient-derived xenograft (PDX) tissues by downregulation an methyltransferase METTL3, contributing to inducation aggressiveness. Mechanistically, characterized forkhead box D3 (FOXD3) as transcription factor for METTL3. F. activates...
DNA comprises molecular information stored in genetic and epigenetic bases, both of which are vital to our understanding biology. Most sequencing approaches address either genetics or epigenetics thus capture incomplete information. Methods widely used detect bases fail common C-to-T mutations distinguish 5-methylcytosine from 5-hydroxymethylcytosine. We present a single base-resolution methodology that sequences complete the two most cytosine modifications workflow. is copied enzymatically...
Paclitaxel (PTX) is one of the most useful chemotherapeutic agents approved for several cancers, including ovarian, breast, pancreatic, and nonsmall cell lung cancer. However, it causes systemic side effects when administered parenterally. Photodynamic therapy (PDT) a new strategy treating local cancers using light photosensitizer. Unfortunately, PDT often followed by recurrence due to incomplete ablation tumors. To overcome these problems, we prepared far-red light-activatable prodrug PTX...
In vitro digestion products of proteins were compared among beef, pork, chicken, and fish. Gastric jejunal contents from the rats fed these meat also compared. Cooked fish homogenized incubated with pepsin alone or followed by trypsin. The molecular weights less than 3000 Da identified MALDI-TOF-MS nano-LC-MS/MS. obtained four for 7 days analyzed. After digestion, beef samples had a greater number fragments in similarity chicken samples, but digestibility was greatest (p < 0.05) pork...
There is currently no optimal scaffold for the transplantation of limbal stem cells (LSCs) to induce corneal reconstruction after alkali burns. This study attempts fabricate a novel in situ Alginate-Chitosan hydrogel (ACH) LSCs transplantation. Sodium alginate dialdehyde (SAD), biological crosslinker, was prepared by periodate-mediated sodium oxidization. Carboxymethyl chitosan rapidly crosslinked with SAD via Schiff's base formation between available aldehyde and amino groups. The ACH...
The stimulator of interferon genes (STING) plays a pivotal role in orchestrating innate immunity, and dysregulated activity STING has been implicated the pathogenesis autoimmune diseases. Recent findings suggest that bacterial infection activates STING, relieving ER stress, triggers non-canonical autophagy by spatially regulating STX17. Despite these insights, precise mechanism governing dynamics autophagosome fusion elicited remains unclear. In this study, we demonstrate dynamic activation...
Early diagnosis of diabetic nephropathy (DN) is difficult although it crucial importance to prevent its development. To probe potential markers and the underlying mechanism DN, an animal model db/db mice, was used serum urine metabolites were profiled using gas chromatography/time-of-flight mass spectrometry. Metabolic patterns evaluated based on data. Principal component analysis data revealed obvious metabonomic difference between mice controls, showed distinctly different metabolic during...
Continuous exposure of breast cancer cells to adriamycin induces high expression P-gp and multiple drug resistance. However, the biochemical process underlying mechanisms for gradually induced resistance are not clear. To explore mechanism evaluate anti-tumor effect adriamycin, drug-sensitive MCF-7S drug-resistant MCF-7Adr were used treated with intracellular metabolites profiled using gas chromatography mass spectrometry. Principal components analysis data revealed that two cell lines...
Abstract Background Heterogeneity of immune gene expression patterns luminal breast cancer (BC), which is clinically heterogeneous and overall considered as low immunogenic, has not been well studied especially in non-European populations. Here, we aimed at characterizing the profile BC an Asian population associating it with patient characteristics tumor genomic features. Methods We performed profiling adjacent normal tissue 92 patients from Hong Kong using RNA-sequencing data used...
Large-scale identification of N-linked intact glycopeptides by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in human serum is challenging because the wide dynamic range protein abundances, lack a complete N-glycan database and existence proteoforms. In this regard, spectral library search method was presented for from glycoproteins target-decoy motif-specific false discovery rate (FDR) control. Serum proteins were firstly separated into low-abundance high-abundance...
PCIF1 (phosphorylated CTD interacting factor 1) is the first reported RNA N6,2'-O-dimethyladenosine (m6Am) methyltransferase. However, pathological significance of and m6Am modification remains unknown. Here we find that both expression are significantly elevated in gastric cancer tissues. Increased associated with progression, predicts poor prognosis. Silence inhibits proliferation invasion cells, suppresses tumor growth metastasis mouse model. m6Am-seq analysis reveals TM9SF1...
Abstract Background While previous genome-wide association studies (GWAS) have identified multiple risk variants for migraine, there is a lack of evidence about how these contribute to the development migraine. We employed an integrative pipeline efficiently transform genetic associations identify causal genes Methods conducted proteome-wide study (PWAS) by combining data from migraine GWAS with proteomic human brain and plasma proteins that may play role in developing also combined novel...
The polysaccharides from Stemona tuberosa Lour, a kind of plant used in Chinese herbal medicine, have various pharmacological activities, such as anti-inflammatory and antioxidant properties. However, the effects extraction methods activity different parts are still unknown. Therefore, this study aimed to evaluate on yields, chemical compositions, bioactivity extracted Lour. Six were leaves, roots, stems Lour through use hot water (i.e., SPS-L1, SPS-R1, SPS-S1) an ultrasound-assisted method...
Aims This study aimed to evaluate the associations between types of night shift work and different indices obesity using baseline information from a prospective cohort workers in China. Methods A total 3,871 five companies were recruited survey. structured self-administered questionnaire was employed collect participants' demographic information, lifetime working history, lifestyle habits. Participants grouped into rotating, permanent irregular groups. Anthropometric parameters assessed by...