A5103207885- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Curcumin's Biomedical Applications
- RNA Interference and Gene Delivery
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Renal and related cancers
- Animal Genetics and Reproduction
- Hedgehog Signaling Pathway Studies
- Biochemical and Molecular Research
- Ovarian function and disorders
- Heat shock proteins research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic and Kidney Cyst Diseases
- Genomics and Chromatin Dynamics
- GDF15 and Related Biomarkers
- RNA modifications and cancer
- Nuclear Receptors and Signaling
- MicroRNA in disease regulation
- Insect Resistance and Genetics
- Circular RNAs in diseases
- Cancer, Stress, Anesthesia, and Immune Response
- Epigenetics and DNA Methylation
- Macrophage Migration Inhibitory Factor
- ATP Synthase and ATPases Research
Guangzhou Institutes of Biomedicine and Health
2024
University of Chinese Academy of Sciences
2013-2024
Guangzhou University of Chinese Medicine
2020-2021
Institute of Zoology
2012-2016
Chinese Academy of Sciences
2012-2016
Mammalian spermatogenesis consists of many cell types and biological processes serves as an excellent model for studying gene regulation at transcriptional post-transcriptional levels. Many key proteins, miRNAs, perhaps piRNAs have been shown to be involved in spermatogenesis. However, a systematic method assessing the relationship between protein mRNA expression has not available mechanisms regulation. In present study, we used iTRAQ-based quantitative proteomic approach identify 2008...
Culture of mouse spermatogonial stem cells (mSSCs) contributes to understanding the mechanisms mammalian spermatogenesis. Several key growth factors such as GDNF and FGF2 have been known be essential for proliferation cultured mSSCs. However, additional regulating SSC remain identified. In this study, we report that IGF-1R signaling is required mSSCs by promoting G2/M progression cell cycle. IGF-1 its receptor are expressed in well isolated Sertoli interstitial cells. Blockage either...
Meiosis is the key step in gametogenesis. However, mechanism of mammalian meiosis remains poorly understood due to lack an vitro model. Here, we report that retinoic acid (RA) sufficient for inducing leptotene/zygotene spermatocytes from cultured mouse spermatogonial stem cells. Multiple genes regulated by RA were identified RNA sequencing. combination with pup Sertoli cell co-culture resulted a higher induction efficiency 28%. Comparisons transcriptomic profiles induced spermatogenic cells...
Abstract The regulatory factor X (RFX) family of transcription factors is crucial for ciliogenesis throughout evolution. In mice, Rfx1-4 are highly expressed in the testis where flagellated sperm produced, but functions these spermatogenesis remain unknown. Here, we report production and characterization Rfx2 knockout mice. male mice were sterile due to arrest at an early round spermatid step. Rfx2- null spermatids detached from seminiferous tubules, forming large multinucleated giant cells...
Matrix Metalloproteinase-26 (MMP-26) is the smallest member of matrix metalloproteinase (MMP) family that functions mainly in extracellular milieu to cleave proteins. It expressed various cell types placenta, but its function placentation has been poorly understood. Considering existence a unique cysteine switch sequence MMP-26 protein sequence, as well evidence cancer cells indicating digestion NH2-terminal domain ERβ by MMP-26, we hypothesize may have specific intracellular activity human...
Breast cancer stem cells are an important cause of radiotherapy resistance in the clinical treatment breast patients. How to target is key improving efficacy radiotherapy. We proposed for first time that curcumin combined with glucose nanogold particles (Glu-GNPs) targeted reduce resistance, which can significantly enhance apoptosis level MCF-7 and MDA-MB-231 stem-like (BCSCs) after antiproliferation colony-forming. Under simulated hypoxic conditions, Glu-GNPs improve ROS mammospheres;...
Basonuclin (BNC1), a zinc finger transcriptional factor, is essential for mouse spermatogenesis. However, the regulatory mechanisms of BNC1 in spermatogenesis are poorly understood. In this study, we identified HSF2BP, testis-specific binding protein HSF2, as partner by using yeast two-hybrid screening. HSF2BP could interact with and inhibit activity without affecting its expression level. Moreover, coexpression resulted striking redistribution to cytoplasm. These data suggest that may play...
Curcumin is a type of plant polyphenol extracted from Curcuma longa L. rhizome, which demonstrates antitumor activity in breast cancer cells vitro. To investigate the combined effect and possible mechanism curcumin glucose-gold nanoparticles (Glu-GNPs), radiosensitivity carcinoma xenografts was assessed nude mice. MDA-MB-231 labeled with firefly luciferase were inoculated into mammary fatty pads mice to establish transplantation tumor model human cancer. The tumor-bearing treated different...
Abstract Breast cancer is the first cause of death in women all over world. And morbidity breast has been increasing recent years. Nowadays, treatment still a problem. Radiotherapy resistance one important factors leading to poor prognosis clinical treatment. Curcumin, plant polyphenol from Curcuma longa, antitumor activity and inhibition tumor recurrence metastasis. However, influence curcumin on radiosensitivity carcinoma xenografts, synergistic effect possible mechanism Glucose-Gold...