Recently, the curcumin derivative CU17 possessing HDAC inhibitory activity has been shown to synergistically enhance anti-proliferative of Gem against lung cancer cells. Nevertheless, mechanism(s) underlying synergistic anti-cancer effect remains be investigated. This study aimed investigate mechanisms that underpin combined and NSCLC A549 cells both in vitro mouse xenograft models. was successfully synthesized subsequently investigated for its combination effects with on inductions cell...

Houttuynia cordata Thunb. has been used as a traditional medicine to treat variety of ailments in Asian countries such China, Japan, South Korea, and Thailand. In Thailand, H. fermentation products (HCFPs) are commercially produced popularly consumed throughout the country without experimental validation. Anti-inflammatory activity fresh leaves or aerial parts previously reported, however, anti-inflammatory available HCFPs by industrialized process not yet investigated. The aim this study...

Previous research reported that the curcumin derivative (CU17) inhibited several cancer cell growths in vitro. However, its anticancer potential against human lung cells (A549 lines) has not yet been evaluated. The purpose of this was to examine HDAC inhibitory and anti-cancer activities CU17 compared (CU) A549 cells. An vitro study showed had greater activity than CU. a dose dependent manner with half-maximal concentration (IC

Houttuynia cordata Thunb. and Phyllanthus emblica Linn. are native plants with medicinal nutritive significance in Asia. The present study was aimed at evaluating antiproliferative effects on human cancer cell lines identifying the phenolic acid composition of water ethanolic extracts powdered formula H. fermented broth P. fruit. Anticancer activity evaluated against HeLa, HT29, HCT116, MCF7 Jurkat cells using an MTT assay flow cytometric analysis apoptosis induction cycle arrest. Reverse...

A number of Houttuynia cordata fermentation products are commercially available in Thailand.However, their anticancer activity remains to be investigated.In this study, the phenolic acid composition and two commercialized H. were investigated.Reversed phase HPLC was used identify quantify acids.MTT Annexin V staining assays investigate antiproliferative apoptosis induction activities, respectively.Seven acids including protocatechuic, p-hydroxybenzoic, vanillic, syringic, p-coumaric,...

Clinical application of cisplatin against cholangiocarcinoma is often associated with resistance and toxicity posing urgent demand for combination therapy. In this study, we evaluated the combined anticancer effect histone deacetylase inhibitors (HDACIs), suberoylanilide hydroxamic acid (SAHA) trichostatin A (TSA), on KKU-100 KKU-M214 cell lines. Antiproliferative activity was using MTT assay. Apoptosis induction cycle arrest were analyzed by flow cytometry. Cell apoptosis regulating...

Chemotherapy with cisplatin in cholangiocarcinoma produces adverse effects and leads to resistance development by tumors. We aimed evaluate anti-cancer co-administration of scopoletin cells. MTT assay, median effect principle, cell cycle arrest apoptosis assay were conducted determine effects. Results revealed that treatment resulted dose-dependent reduction viability for Combination these agents inhibited proliferation cells significantly more than single agent either. indices reflect...

This study investigated the impact of Valencia KK4-type peanut skin ethanolic extract (KK4-PSE) combined with cisplatin or 5-fluorouracil (5-FU) on HeLa cells in vitro and xenograft models. At exposure times 24, 48 72 h, KK4-PSE inhibited growth a half maximal inhibitory concentration (IC

Background and Objectives: The treatments of cholangiocarcinoma (CCA) with Cisplatin (Cis) Gemcitabine (Gem) often cause side effects drug resistance. This study aimed to investigate the combined Tiliacora triandra leaf powder ethanolic extract (TLPE) Cis or Gem on CCA cells in vitro nude mouse xenografts. Materials Methods: Antiproliferative activity was evaluated using MTT assay. Drug interaction studied by Chou-Talalay method. Apoptosis induction cell cycle arrest were analyzed flow...

Abstract Application of 5-fluorouracil (5-FU) in cholangiocarcinoma (CCA) is limited by adverse side effects and chemoresistance. Therefore, the combination therapy 5-FU with other substances, especially natural products may provide a new strategy for CCA treatment. The aim this study was to evaluate two ethanolic extracts Thai noni juice (TNJ) on cell lines nude mice xenografts. results antiproliferative assay showed treatment each TNJ extract exerted more cytotoxicity cells than either...

Background: Recent attention on chemotherapeutic intervention against cancer has been focused discovering and developing phytochemicals as anticancer agents with improved efficacy, low drug resistance toxicity, cost limited adverse side effects. In this study, we investigated the effects of Curcuma C20-dialdehyde growth, apoptosis cell cycle arrest in colon cervical lines. Materials Methods: Antiproliferative, induction, activities were determined by WST proliferation assay, flow cytometric...

Twenty newly synthesized derivatives of [6]-shogaol (4) were tested for inhibitory activity against histone deacetylases. All showed moderate to good deacetylase inhibition at 100 µM with a slightly lower potency than the lead compound. Most potent inhibitors among pyrazole products, 5j and 5k, Michael adduct pyridine 4c benzothiazole 4d, IC50 values 51, 65, 61 60 µM, respectively. They further evaluated isoform selectivity via molecular docking study. Compound 4d best towards HDAC3, whereas...

Abstract: This study aimed at investigating the effects of Valencia KK4-type peanut skin ethanolic extract (KK4-PSE) in combination with cisplatin or 5-fluorouracil (5-FU) against HeLa cells vitro and xenograft models. MTT assay showed that KK4-PSE, cisplatin, 5-FU suppressed proliferation a dose time dependent manner. Drug interactions by Chou-Talalay method demonstrated KK4-PSE enhanced antiproliferative activity index (CI) values 0.49 (48 h) 0.60 (72 h), indicating synergistic effect,...

Houttuynia cordata fermentation products (HCFPs) are produced and widely used as dietary supplements for health immune support. However, the effect on function these has not been clearly demonstrated. In this study, soluble fractions of selected HCFP were determination immunomodulatory potential, both in vitro animal models. Viability proliferation rat splenocytes phagocytic activity human neutrophils evaluated. Studies effects, including hematological parameters, mitogen-driven lymphocyte...

Houttuynia cordata Thunb. fermentation product (HCFP) is widely used in Thailand as a dietary supplement for immune support with no experimental verification. The aims of this study were to investigate the safety and immunomodulation HCFP healthy volunteers its effect on antiretroviral drugs Sprague Dawley rats. basic characteristics blood chemistry 10 did not show any significant differences between before after 4 weeks intervention daily intake HCFP, major adverse event was observed....

This work revealed peanut seed prolamins likely displaying a defensive role besides the known nitrogen storage. Drought stress and proteomic approaches were used in varieties of peanuts to explore prolamin member association with test against Aspergillus flavus spore germination. The effect was showed by aerial biomass, leaf content malondialdehyde, contamination A. flavus. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis profiles not informative for antifungal polypeptides. From...