A5059704901- Retinal Diseases and Treatments
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Retinal Imaging and Analysis
- Ocular Surface and Contact Lens
- Cell Adhesion Molecules Research
- Corneal surgery and disorders
- Retinal and Optic Conditions
- Intraocular Surgery and Lenses
- Ocular Diseases and Behçet’s Syndrome
- Connexins and lens biology
- Lung Cancer Treatments and Mutations
- Heart Failure Treatment and Management
- Retinal Development and Disorders
- Ocular Infections and Treatments
- Protease and Inhibitor Mechanisms
- Advanced Glycation End Products research
- Cellular Mechanics and Interactions
- Cancer, Hypoxia, and Metabolism
- Immune Response and Inflammation
- Retinal and Macular Surgery
- Cancer Diagnosis and Treatment
- Lung Cancer Diagnosis and Treatment
- Lung Cancer Research Studies
- NF-κB Signaling Pathways
Yamaguchi University
2016-2025
Shinshu University
2010-2025
Tosei General Hospital
2024
Shinshu University Hospital
2018-2021
Shizuoka University
2021
Matsumoto University
2021
Ophthalmology Associates (United States)
2019
Shionogi (Japan)
2002-2018
Ōtani University
2018
Japan Meteorological Agency
2018
view Abstract Citations (402) References (37) Co-Reads Similar Papers Volume Content Graphics Metrics Export Citation NASA/ADS X-Ray Variability of GX 339-4 in Its Very High State Miyamoto, Sigenori ; Kimura, Kazuhiro Kitamoto, Shunji Dotani, Tadayasu Ebisawa, Ken Results are presented on observations 339 - 4 performed with the large-area counters aboard Ginga when this X-ray source was its very high state. It shown that energy spectrum consists a low-energy component, interpreted as being...
The small GTPase Rho acts on two effectors, ROCK and mDia1, induces stress fibers focal adhesions. However, how mDia1 individually regulate signals dynamics of these structures remains unknown. We stimulated serum-starved Swiss 3T3 fibroblasts with LPA compared the effects C3 exoenzyme, a inhibitor, those Y-27632, inhibitor. Y-27632 treatment suppressed LPA-induced formation adhesions as did exoenzyme but induced membrane ruffles complexes, which were absent in exoenzyme-treated cells. This...
Thirty patients from 15 to 69 years of age with congenital cystic dilatation the common bile duct were studied. The diagnosis was made by intravenous cholangiography in 70% and percutaneous transhepatic and/or endoscopic retrograde cholangiopancreatiography entire group. Cystic also noted intraphepatic ducts 12 patients. A union between main pancreatic occurred at a high position 17 18 whom both adequately opacified, forming an abnormally long channel. One patient choledochodele had normal...
Background & AimsPlatelet transfusion is used to prevent hemorrhagic events in patients with thrombocytopenia undergoing invasive procedures, but there are many disadvantages. We evaluated the efficacy and safety of lusutrombopag chronic liver disease procedures.MethodsWe performed a double-blind, parallel-group, phase 3 study 96 (platelet counts below 50,000/μL) procedures from October 2013 May 2014 at 81 centers Japan. Patients were randomly assigned (1:1) groups given once-daily (3 mg) or...
The molecular mechanisms behind phenotypic modulation of smooth muscle cells (SMCs) remain unclear. In our recent paper, we reported the establishment novel culture system gizzard SMCs (Hayashi, K., H. Saga, Y. Chimori, K. Kimura, Yamanaka, and Sobue. 1998. J. Biol. Chem. 273: 28860–28867), in which insulin-like growth factor-I (IGF-I) was most potent for maintaining differentiated SMC phenotype, IGF-I triggered phosphoinositide 3-kinase (PI3-K) protein kinase B (PKB(Akt)) pathway. Here,...
Under conventional culture conditions, smooth muscle cells display their phenotypic modulation from a differentiated to dedifferentiated state. Here, we established primary system of maintaining phenotype, as characterized by expression muscle-specific marker genes such h-caldesmon and calponin, cell morphology, ligand-induced contractility. Laminin retarded the progression dedifferentiation cells. Insulin-like growth factors (IGF-I IGF-II) insulin markedly prolonged with IGF-I being more...
We developed a new pull-down assay for GTP-Rho and examined its level during cell cycle. HeLa cells were arrested in the S phase by thymidine enriched prometaphase, metaphase, telophase, G(1) collecting at 0, 45, 90, 180 min after release from nocodazole arrest, respectively. The of did not change significantly to but increased thereafter, peaking returned original phase. GDP-GTP exchange activity Rho measured lysates parallel also mitosis with peak metaphase. Using this system, we role...
TNF-α disrupts the barrier function of cultured human corneal epithelial (HCE) cells. We investigated effects cytoprotective drug rebamipide on this disruption by as well damage in a rat model dry eye.The HCE cells was evaluated measurement transepithelial electrical resistance. The distribution tight-junction (ZO-1, occludin) and adherens-junction (E-cadherin, β-catenin) proteins, p65 subunit nuclear factor-κB (NF-κB) determined immunofluorescence microscopy. Expression junctional proteins...
Purpose.: To characterize the structural properties of collagen lamellae in normal and keratoconic human corneal stroma, we measured their width angle relative to Bowman's layer (BL). Methods.: Thirteen four corneas were examined. Collagen tissue blocks from central cornea visualized by second harmonic generation imaging microscopy. Images obtained 1-μm steps BL Descemet's membrane (DM) subjected three-dimensional reconstruction. The reconstructed data sets divided into 10 layers equal depth...
Although Rho regulates cytokinesis, little was known about the functions in mitosis of Cdc42 and Rac. We recently suggested that works metaphase by regulating bi-orient attachment spindle microtubules to kinetochores. now confirm role RNA interference identify mechanisms for activation down-regulation Cdc42. Using a pull-down assay, we found level GTP-Cdc42 elevates metaphase, whereas GTP-Rac does not change significantly mitosis. Overexpression dominant-negative mutants Ect2 MgcRacGAP,...
The fluctuations in the expression of two forms DNA topoisomerase II, p170 and p180, were examined by immunoblotting during transition growth state cell cycle Swiss 3T3 cells. level was higher exponentially growing phase than stationary phase, p180 did not appear to change when cells changed state. When quiescent stimulated with serum, began increase at 12 h reached a maximal 24-28 h, corresponding G2 phase. In contrast, almost constant cycle, band became obscure number mitotic increased....
purpose. The corneal epithelium provides a barrier that is both important for homeostasis and dependent on tight junctions (TJs) between adjacent epithelial cells. authors examined the effects of tumor necrosis factor-α (TNF-α), proinflammatory cytokine, function expression TJ proteins in simian virus 40–transformed human (HCE) methods. cultured HCE cells was evaluated by measurement transepithelial electrical resistance (TER). subcellular distribution zonula occludens-1 (ZO-1) occludin p65...
The barrier function of the corneal epithelium contributes to homeostasis and is impaired by inflammation. Adherens junctions (AJs) tight (TJs) are essential for cell adhesion function. We examined effects proinflammatory cytokine interleukin (IL)-1beta on AJs TJs as well in simian virus 40-transformed human epithelial (HCE) cells.Barrier was evaluated measurement transepithelial electrical resistance (TER). subcellular distributions AJ proteins E-cadherin beta-catenin, TJ ZO-1 occludin, p65...
In this study, we investigated the therapeutic potential of a Rho-associated coiled-coil-containing protein kinase (ROCK) inhibitor ripasudil (K-115) eye drop on retinal neovascularization and hypoxia.In vitro, human microvascular endothelial cells (HRMECs) were pretreated with then stimulated VEGF. ROCK activity was evaluated by phosphorylation myosin phosphatase target (MYPT)-1. Endothelial migration cell viability assessed MTT assay, respectively. The concentration in retina measured...
ABSTRACT Mechanical stimulation as a mimic of drusen formation in the eye increases expression angiogenic factors retinal pigment epithelial (RPE) cells, but underlying molecular mechanisms remain unclear. We investigated and characterized effects mechanical on RPE cells both vitro mouse model. increased vascular endothelial growth factor (VEGF, encoded by VEGFA) other angiogenesis-related genes cultured RPE1 cells. The presence hypoxia-inducible 1α (HIF-1α, HIF1A) was also increased,...
It has been demonstrated that proinsulin C-peptide possesses several biological activities and its specific binding sites are present on the surface of cell membranes. However, molecular cellular mechanisms actions poorly known. In study we examined possible involvement mitogen-activated protein kinase (MAPK) pathway in effects. induced phosphorylation MAPK [p44 extracellular signal-regulated 1 (ERK1) p42 ERK2] Swiss 3T3 3T3-F442A fibroblasts but not 3T3-L1 some other lines such as L6E9...
purpose. Polyinosinic-polycytidylic acid [poly(I:C)], an analog of viral double-stranded RNA, interacts with Toll-like receptor (TLR)-3 and thereby elicits immunoinflammatory responses characteristic infection. The effects poly(I:C) on the expression proinflammatory cytokines, chemokines, adhesion molecules, as well signaling pathways that underlie such effects, were investigated in cultured human corneal fibroblasts. methods. Expression molecules intercellular molecule (ICAM)-1 vascular...
Purpose.: Migration of corneal epithelial cells is an important step in the wound healing. The role extracellular signal regulated kinase (ERK) for regulation cell migration during closure was examined. Methods.: Scratch wounds were introduced into human absence or presence PD98059, ERK signaling inhibitor. phosphorylation and localization examined by immunoblot immunofluorescence analyses. tyrosine focal adhesion (FAK) paxillin, as well association FAK with paxillin ERK, evaluated...
Background and Purpose— Several studies suggested that statins during hospitalization were associated with better disability outcomes in patients acute ischemic stroke, but only 1 small randomized trial is available. Methods— We conducted a multicenter, open-label, controlled strokes 11 hospitals Japan. Patients stroke dyslipidemia randomly received within 24 hours after admission the early group or on seventh day delayed group, 1:1 ratio. Statins administered for 12 weeks. The primary...
The contemporary outcome of balloon pulmonary angioplasty (BPA) and endarterectomy (PEA) in patients with chronic thromboembolic hypertension (CTEPH) are unclear.
Conventional hepatic arteriography combined with superselective infusion was carried out in 51 patients hepatocellular carcinoma smaller than 5 cm, and angiograms of varying phases were analyzed. In cancers 40 mm, particularly those 20 so-called tumor stain the capillary phase only abnormality seen most but not all cases. Within a stain, there unstained areas cases histologic examination resected specimens showed them to be due either necrosis, fibrosis, or fatty changes. Homogeneity shape...