A5027934348- Immunotherapy and Immune Responses
- Prenatal Screening and Diagnostics
- RNA Interference and Gene Delivery
- Genomics and Rare Diseases
- MicroRNA in disease regulation
- Cancer Immunotherapy and Biomarkers
- Genetics and Neurodevelopmental Disorders
- Genomic variations and chromosomal abnormalities
- Virus-based gene therapy research
- Cancer Mechanisms and Therapy
- Cancer-related molecular mechanisms research
- Fetal and Pediatric Neurological Disorders
- Immune Cell Function and Interaction
- Cancer-related Molecular Pathways
- CAR-T cell therapy research
- Prostate Cancer Treatment and Research
- Parvovirus B19 Infection Studies
- Cancer therapeutics and mechanisms
- Liver physiology and pathology
- Cutaneous Melanoma Detection and Management
- Cancer Cells and Metastasis
- Cell Adhesion Molecules Research
- Congenital heart defects research
- Circular RNAs in diseases
- Microtubule and mitosis dynamics
Dalian Medical University
2021-2025
Chongqing Medical University
2021-2025
Second Affiliated Hospital of Chongqing Medical University
2021-2025
Second Affiliated Hospital of Zhejiang University
2025
Huazhong University of Science and Technology
2025
Guangzhou University of Chinese Medicine
2025
Foshan Hospital of TCM
2025
Kunming Medical University
2009-2024
Wuhan University
2024
Renmin Hospital of Wuhan University
2024
To report the performance of massively parallel sequencing (MPS) based prenatal noninvasive fetal trisomy test on cell-free DNA from maternal plasma in a routine clinical setting China.The MPS-based was offered as screening for trisomies 21 and 18 to pregnant women 49 medical centers over 2 years. A total 11,263 participants were recruited performed 11,105 pregnancies. Fetal outcome data obtained after expected date confinement.One hundred ninety cases classified positive, including 143 47...
Abstract Enhancer of zeste homolog 2 (EZH2) has been shown to be overexpressed in hepatocellular (HCC). We investigated the potential role EZH2 HCC tumorigenesis and examined usefulness RNA interference (RNAi) targeting as a form treatment. Lentivirus-mediated RNAi was employed knock-down expression human hepatoma cells study function evaluate treatment efficacy. effectively reduced expression. Suppression significantly their growth rate vitro markedly diminished tumorigenicity vivo....
The emerging concept of generating cancer stem cells from epithelial-mesenchymal transition has attracted great interest; however, the factors and molecular mechanisms that govern this putative tumor-initiating process remain largely elusive. We report here miR-200a not only regulates but also stem-like in nasopharyngeal carcinoma cells. first showed stable knockdown promotes epithelium-like CNE-1 to mesenchymal phenotype. More importantly, it induced several cell-like traits, including...
Balanced chromosomal rearrangement (or balanced chromosome abnormality, BCA) is a common structural variation. Next-generation sequencing has been reported to detect BCA-associated breakpoints with the aid of karyotyping. However, complications associated this approach and requirement for cytogenetics information limited its application. Here, we provide whole-genome low-coverage BCA events independent knowing affected regions low false positives. First, six samples containing BCAs were used...
Hepatocellular carcinoma (HCC) is one of the leading causes cancer-related deaths worldwide. Surgical resection and conventional chemotherapy radiotherapy ultimately fail due to tumor recurrence HCC's resistance. The development novel therapies against HCC thus urgently required. cyclin-dependent kinase (CDK) pathways are important well-established targets for cancer treatment. In particular, CDK2 a key factor regulating cell cycle G1 S transition hallmark cancers. this study, we utilized...
The identification of causative mutations is important for treatment decisions and genetic counseling patients with disorders sex development (DSD). Here, we designed a new assay based on targeted next-generation sequencing (NGS) to diagnose these genetically heterogeneous disorders. All coding regions flanking sequences 219 genes implicated in DSD were be included panel. A total 45 samples used chromosome dosage validation by using the NGS platform. Among these, 21 processed find mutation....
Mucosal melanoma (MM) is a highly vascularized tumor with an extremely poor prognosis. In this randomized, open-label, phase II study, we characterized the efficacy and safety of bevacizumab in combination carboplatin plus paclitaxel (CPB) patients previously untreated advanced MM.Patients were randomly assigned 2:1 ratio to receive (area under curve, 5) (175 mg/m2) once every 4 weeks (CPB arm, 5 mg/kg) or without (CP arm) 2 weeks. Progression-free survival (PFS) was primary end point....
The human microRNA cluster MC-let-7a-1∼let-7d, with three members let-7a-1, let-7f-1, and let-7d, is an important of the let-7 family. These microRNAs play critical roles in regulating development carcinogenesis. Therefore, precise control MC-let-7a-1∼let-7d level for cellular functions. In this study, we first showed that expression these was significantly reduced hepatocellular carcinoma HepG2 cells as compared immortalized liver L02 cells. We demonstrated encoded by a single polycistronic...
Cyclin-dependent kinase 2 (CDK2) has been reported to be overexpressed in human colorectal cancer; it is responsible for the G1‑to‑S‑phase transition cell cycle and its deregulation a hallmark of cancer. The present study was first use idock, free open‑source protein‑ligand docking software developed by our group, identify potential CDK2 inhibitors from 4,311 US Food Drug Administration‑approved small molecular drugs with re‑purposing strategy. Among top compounds identified idock score,...
Abstract Background The clinicopathological and survival profiles across primary sites in acral melanoma (AM) are still controversial unclear. Methods This is a multi-center retrospective study. Clinicopathological data of AM patients diagnosed between 1 January 2000 31 December 2017 from 6 large tertiary hospitals China were extracted. Chi square tests used to compare basic characteristics sole, palm nail bed. Melanoma-specific (MSS) differences based on compared by log-rank multivariate...
Interleukin-2 (IL-2) has been shown to possess antitumor activity in numerous preclinical and clinical studies. However, the short half-life of recombinant IL-2 protein serum requires repeated high-dose injections, resulting severe side effects. Although adenovirus-mediated gene therapy efficacy, host antibody response adenoviral particles potential biosafety concerns still obstruct its applications. Here we report a novel nanopolymer for delivery, consisting low molecular weight...
To evaluate the performance of a massively parallel sequencing (MPS)-based test in detecting fetal sex chromosome aneuploidy (SCA) and to present comprehensive clinical counseling protocol for SCA-positive patients.This was retrospective study large patient cohort 5950 singleton pregnancies which underwent MPS-based testing as prenatal screening trisomies 21, 18 13, with X Y chromosomes secondary findings, Southwest Hospital China. women were offered choice knowing whether their SCA results...
Hepatocellular carcinoma (HCC) is a hypervascular cancer without effective treatment. Here we report that polypeptide of NC1 domain type VIII collagen (Vastatin) an endogenous expressed in normal liver tissue but lost the most HCC patients (73.1%). Its expression level negatively associated with tumor size (P = 0.035) and metastasis 0.016) patients. To evaluate its potential use as therapeutic, constructed recombinant adeno-associated virus carrying Vastatin (rAAV-Vastatin) to treat...
Chimeric antigen receptor T cell (CAR-T) therapy has achieved remarkable effects in refractory/relapsed (R/R) diffuse large B-cell lymphoma (DLBCL). However, many patients receiving CAR-T still eventually die of disease recurrence or progression due to target loss exhaustion cells. Therefore, maintaining the efficacy become a particular research focus. As lenalidomide can regulate function, we conducted study evaluate maintenance after R/R DLBCL patients.Seven who received and nine that...
Hepatocellular carcinoma (HCC) is one of the most common and malignant cancers. The HCC incidence gets a strong sexual dimorphism as men are major sufferers in this disaster. Although several studies have uncovered presentative correlation between axis androgen/androgen receptor (AR) incidence, mechanism still largely unknown. Cancer stem cells (CSCs) small subgroup cancer contributing to multiple tumors behaviors, which play an important role oncogenesis various cancers including HCC....
Renal carcinoma presents a rapid progression in patients with high metastasis no effective therapeutic strategy. In this study, we designed folate-grafted PEI600-CyD (H1) nanoparticle-mediated DNA vaccine containing an adjuvant of absent melanoma 2 (AIM2) and tumor-specific antigen carbonic anhydrase IX (CAIX) for renal therapy. Mice bearing subcutaneous human CAIX (hCAIX)-Renca tumor were intramuscularly immunized H1-pAIM2/pCAIX, H1-pCAIX, H1-pAIM2, or Mock vaccine, respectively. The growth...