A5091270742- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Outbreaks Research
- vaccines and immunoinformatics approaches
- SARS-CoV-2 detection and testing
- Biosimilars and Bioanalytical Methods
- Diagnosis and treatment of tuberculosis
- COVID-19 Clinical Research Studies
- Child Nutrition and Water Access
- Infectious Diseases and Tuberculosis
- Retinoids in leukemia and cellular processes
- Biosensors and Analytical Detection
- Clinical Reasoning and Diagnostic Skills
- Pregnancy and preeclampsia studies
- Bacterial Identification and Susceptibility Testing
- Neonatal Respiratory Health Research
- COVID-19 diagnosis using AI
- Cell Image Analysis Techniques
- Cervical Cancer and HPV Research
- Pneumocystis jirovecii pneumonia detection and treatment
- Evolution and Genetic Dynamics
- HIV Research and Treatment
Université des Sciences, des Techniques et des Technologies de Bamako
2016-2023
Background: To identify strains of Mycobacterium tuberculosis complex (MTBc) circulating in Bamako region during the past 10 years. Methods: From 2006 to 2016, we conducted a cross-sectional study with spoligotyping, clinical isolates from (TB)-infected patients at different stages their treatments Bamako, Mali. Results: Among 904 suspected TB included and thereafter tested our BSL-3 laboratory, 492 (54.4%) had MTBc therefore underwent spoligotyping. Overall, three subspecies, i.e., MTB T1...
Background: While, bacteria resistance mutations can affect competitive fitness, given our multidrug-resistant (MDR) prevalence, we conducted this study to determine the impact of MDR on fitness Mycobacterium tuberculosis (MTB) complex strains. We a cross-sectional at University Clinical Research Center (UCRC) from January December 2017. New TB patients over aged 18 were recruited teaching hospital and health reference centers Bamako in USTTB Ethical committee approved protocols. Methods:...
The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) variants may have contributed to prolonging the pandemic, and increasing morbidity mortality related coronavirus disease 2019 (COVID-19). This article describes dynamics circulating SARS-CoV-2 identified during different COVID-19 waves in Mali between April October 2021.The complete spike (S) gene from positive samples was sequenced. Generated sequences were aligned by Variant Reporter v3.0 using Wuhan-1 strain as...
Background: Despite recent advances in the development of more sensitive technologies for diagnosis tuberculosis (TB), resource-limited settings, continues to rely on sputum smear microscopy. This is because microscopy simple, cost-efficient and most accessible tool TB. Our study evaluated performance light-emitting diode fluorescence (LED-FM) using auramine/rhodamine (auramine) fluorescein di-acetate (FDA) vital stain diagnostic pulmonary TB Bamako, Mali. Methods: Sputum was conducted FDA...
Pulmonary tuberculosis (TB) remains one of the main causes morbidity and mortality in Mali. Nontuberculous mycobacteria (NTM) infections are very common but often cofounded with TB because similarity symptoms, which makes diagnosis difficult. Hematological abnormalities associated have been described, not NTM. Therefore, goal this study was to compare hematological parameters patients infected NTM infections. A cross-sectional enrolling participants conducted 2018-2020. Five milliliters...
Diabetes Mellitus (DM) increases worldwide, mostly in low- and middle-income countries. In Mali, the prevalence adult population is estimated at 1.8%, but tuberculosis (TB) patients are not systematically screened. The goal of our study was to determine DM among newly diagnosed TB patients.We conducted a cross sectional pilot prospective cohort four health centers Bamako. All underwent fasting capillary-blood glucose (FCBG) test Day 0, repeated after one-week treatment. Venous FBG performed...
The diagnosis of tuberculosis (TB) has mostly been relied on a long-used method called sputum smear microscopy. In 2010, Xpert MTB/RIF assay was approved by the World Health Organization for simultaneous TB and detection resistance. Our current study undertaken to compare diagnostic performance auramine staining-based light-emitting diode-Fluorescence Microscopy (LED-FM) considering culture as gold standard pulmonary extrapulmonary TB.Pulmonary specimens suspected patients were examined in...
Ability to rapidly and accurate diagnose pathogens during disease outbreaks is essential for public health. Diagnosis depends largely on laboratory capacity, which can be challenging in resource limited settings. We report Mali’s experience involving four research laboratories response the COVID-19 pandemic. This coordinated effort leveraged emerging infectious diagnostic capacity partnerships built from 2014/2015 Ebola outbreak. Since first two cases March 2020, 349,292 suspected were...
The recent call for universal drug susceptibility testing (DST) all tuberculosis (TB) patients will be difficult to meet in settings where Xpert rollout is limited, such as low prevalence of HIV and Multi-drug Resistant Tuberculosis (MDR) settings. As recommended by World Health Organization (WHO) guidelines, the success TB treatment measured Ziehl–Neelsen (ZN) microscopy or auramine–rhodamine fluorescent (FM) on sputum, which conversion negative smear at 2 months (M) an important predictor...
BACKGROUND: Non-conversion on auramine smear microscopy indicates a lack of treatment response, possibly associated with initial rifampicin-resistant tuberculosis (RR-TB). However, dead bacteria still stain positive and may be detected. Fluorescein diacetate (FDA) shows live mycobacteria only. Therefore, we studied the potential 2-month (2M) FDA for identification RR-TB. METHODS: Between 2015 2018, enrolled new smear-positive pulmonary TB patients from five local centres in Bamako, Mali....
Introduction: While real-time reverse transcription PCR (RT-PCR) is the recommended laboratory method to diagnose severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) infection, its use in resource limited settings can be difficult maintain due high testing demand and shortage of reagents. The aim this study was evaluate performances Realy Tech™ Standard Q™ comparison RT-PCR a relatively low COVID-19 prevalence setting, Mali.Methods: We conducted cross-sectional between January April...
Sputum smear microscopy (SSM), the most widely available tool for tuberculosis (TB) detection, has limited performance in paucibacillary patients and requires highly experienced technicians. The objective of this study was to determine whether addition sodium dodecyl sulfate (SDS), a detergent that thins sputum, at 4% 10%, improves detection acid-fast bacilli (AFB), clarity slides, biosafety technique. Thirty participants with presumptive TB were enrolled. Three independent, blinded...
Background: Cervical cancer (CC) is a major public health problem worldwide.In Senegal, it the first female before breast cancer.Human papillomavirus (HPV) has been described as responsible for CC and most common sexually transmitted infection in world.Low risk HPV (HPV-LR) including 6 11 are of mild infections while among high-risk (HPV-HR) genotypes 16,18, 45,31,33, 52, 58,35 order frequency involved almost 90% cervical cancer.Objective: The general objective to do molecular genotyping...
Introduction Xpert MTB/RIF assay is used extensively for the detection of rifampicin-resistant TB (RR-TB). RR-TB treatment monitoring culture-based, although, in resource-limited settings, access to culture poor. Alternative methods are needed. We therefore conducted a pilot study determine performance fluorescein di-acetate FDA vital staining, microscopy-based test that shows viable bacilli, and threshold cycle value (Ct) changes when assessing conversion at end intensive phase treatment....
The objective of this study was to determine the epidemiological profile preterm infants followed at Kangaroo Unit. Patients and Methods: This a descriptive cross-sectional carried out in Pediatrics Department Sikasso hospital (Mali), second-reference from 1 January 2019 31 December on comprehensive sample all unit during period. Results: We collected 94 files 194, frequency 48.4%. average age mothers 28.5 years 92, 5% were married, out-of-school represented 51%. Housewives majority with...