A5021793012- HIV/AIDS Research and Interventions
- Antibiotic Resistance in Bacteria
- HIV Research and Treatment
- SARS-CoV-2 and COVID-19 Research
- HIV, Drug Use, Sexual Risk
- Viral Infections and Outbreaks Research
- Escherichia coli research studies
- Pneumonia and Respiratory Infections
- Enterobacteriaceae and Cronobacter Research
- HIV-related health complications and treatments
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Infections and bacterial resistance
- Bacterial Infections and Vaccines
- Bacterial Identification and Susceptibility Testing
- HIV/AIDS drug development and treatment
- SARS-CoV-2 detection and testing
- Tuberculosis Research and Epidemiology
- Hepatitis B Virus Studies
- Healthcare Systems and Practices
- Salmonella and Campylobacter epidemiology
- Streptococcal Infections and Treatments
- COVID-19 diagnosis using AI
- Antibiotic Use and Resistance
- Clinical Laboratory Practices and Quality Control
Institut National de Recherche en Santé Publique
2014-2024
University of Bamako
2022-2024
Centre Pour le Développement des Vaccins-Mali
2024
Institut National de Santé Publique
2021-2024
Université des Sciences, des Techniques et des Technologies de Bamako
2015-2023
University Hospital Medical Center at Treichville
2018
Cheikh Anta Diop University
2010-2015
Shigella is a major cause of diarrhea in young children worldwide. Multiple vaccines targeting are development, and phase 3 clinical trials imminent to determine efficacy against shigellosis. The Enterics for Global Health (EFGH) surveillance study designed the incidence medically attended shigellosis 6- 35-month-old 7 resource-limited settings. Here, we describe microbiological methods used isolate identify Shigella. We developed standardized laboratory protocol isolation identification by...
Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella clinical samples compared culture-based methods, which underestimate burden by 2- 3-fold. qPCR assays have also developed for speciation and serotyping, is critical both vaccine development evaluation. The Enterics Global Health (EFGH) surveillance study will utilize a customized real-time PCR-based TaqMan Array Card (TAC) interrogating 82 targets, the detection...
The worldwide dissemination of extended-spectrum beta-lactamase producing Enterobacteriaceae, (ESBL-E) and their subset carbapenemases (CPE), is alarming. Limited data on the prevalence such strains in infections from patients Sub-Saharan Africa are currently available. We determined, here, ESBL-E/CPE bacteriemic two teaching hospitals Bamako (Mali), which at top health care pyramid country. During one year, all Enterobacteriaceae isolated bloodstream (E-BSI), were collected hospitalized...
Abstract Background Diarrhoea is a public health problem, especially in developing countries where it the second leading cause of child mortality. In Low Income Countries like Mali, self-medication and inappropriate use antibiotics due to scarcity complementary diagnostic systems can lead development multidrug-resistant bacteria causing diarrhoea. The objective this work was determine microorganisms responsible for diarrhoea children under 15 years age characterize their sensitivity panel...
Introduction Access to antiretroviral treatment (ART) becomes more and effective in resource‐limited settings (RLS). However, this global effort would be even profitable if the access laboratory services especially decentralized was strengthened. We report virological outcome HIV‐1 drug resistance three West African countries using dried blood spots (DBS) samples. Methods included HIV‐1‐infected adults on ART ≥6 months followed up capital cities sites Senegal, Mali Guinea‐Conakry. Patients...
Integrase inhibitors (INIs) are a potent option for HIV treatment. Limited data exist on INI resistance in West Africa, particularly children living with HIV/AIDS. We determined the prevalence of integrase gene polymorphisms and frequency naturally occurring amino acid (aa) substitutions at positions associated resistance. Dried blood spot (DBS) samples were obtained from one hundred seven (107) HIV-1-infected aged less than 15 years old two African countries, Benin Mali. All naïve to...
The increasing frequency of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a serious public health concern. This study sought to determine ESBL in isolated from patients' blood cultures two university teaching hospitals Bamako, Mali.During three-month period, the presence patients admitted Bamako was evaluated. microbial identifications were initially performed with an API 20E gallery and VITEK2 locally Mali, then confirmation France mass spectrometry...
In 2016, Mali reported a bacterial meningitis outbreak consisting of 39 suspected cases between epidemiologic weeks 9 and 17 with 15% case fatality ratio in the health district Ouéléssebougou, 80 kilometers from capital Bamako. Cerebrospinal fluid specimens 29 were tested by culture real-time polymerase chain reaction; 22 (76%) positive for pathogens, 16 (73%) which Neisseria meningitidis (Nm). Of Nm-positive specimens, 14 (88%) N serogroup C (NmC), 1 was NmW, nongroupable. Eight NmC...
Antimicrobial resistance (AMR) is a global health concern, with extended-spectrum β-lactamases (ESBLs) and carbapenemases being major contributors. Pleural infection (PI) severe condition in West Africa, complicated by AMR. This study aimed to investigate the prevalence molecular characteristics of ESBL carbapenemase-producing enterobacteria pleural effusions Mali.Pleural fluid samples from 526 patients pleuritis were analyzed. Enterobacterial species isolated identified, genes (blaOXA-48,...
Hepatitis B is a major public health problem in Senegal, country with high prevalence and transmission occurring mainly during infancy. Only, one 6-8 weeks vaccination campaign was initiated 2005 it part of the expanded program immunization. The aim this study to determine HBsAg children born from HIV-seropositive mothers by using dried blood specimens. Specimens were collected between July 2007 November 2012 aged 2-48 Dakar decentralized sites working on HIV mother-to-child prevention....
Objectif : La transmission mère-enfant du VIH durant l’allaitement est un problème de santé publique. Le but cette étude était d’évaluer l’intérêt suivi thérapeutique pharmacologique des femmes allaitantes sous ARV et leurs enfants à Bamako. Méthodes Les patientes ont été recrutées 1er septembre 2015 au 30 2016 suite l’obtention leur consentement éclairé. mères enfant suivis dans le service pédiatrie CHU Gabriel Touré. charges virales plasmatiques réalisées l’UCRC détermination...
Praziquantel is the basic drug recommended for Mass Drug Administration (MDA) by World Health Organization (WHO). However, its impact on prevalence of infection has not been updated after a decade MDA in Mali. This study aimed to assess and intensity urogenital schistosomiasis schoolage children Kayes region. A community-based cross-sectional was conducted three health districts (Bafoulabe, Diema, Oussoubidiagnan) from June August 2022. School-age ranging 5 14 years were enrolled at home...
Underdeveloped and underused medical laboratories in sub-Saharan Africa negatively affect the diagnosis appropriate treatment of ailments.We identified political, disease-related socio-economic factors that have shaped laboratory sector Senegal, Mali Burkina Faso to inform laboratory-strengthening programmes.We searched peer-reviewed grey literature from February 2015 December 2018 on health systems development colonial times present conducted in-depth interviews with 73 key informants...
The use of antimicrobials without microbiological proof is frequent and contributes to the emergence resistance. aim was identify organisms isolated during laboratory examinations type consumed by patients hospitalized Infectious Diseases’ Clinic. This a cross-sectional analytical study, carried out from January 1 December 31, 2017 in Diseases Department Point “G” Teaching Hospital Mali. All biological specimens study period were analyzed. In total, 322 benefited test, with mean age 40.9 ±...
Ability to rapidly and accurate diagnose pathogens during disease outbreaks is essential for public health. Diagnosis depends largely on laboratory capacity, which can be challenging in resource limited settings. We report Mali’s experience involving four research laboratories response the COVID-19 pandemic. This coordinated effort leveraged emerging infectious diagnostic capacity partnerships built from 2014/2015 Ebola outbreak. Since first two cases March 2020, 349,292 suspected were...
Tuberculosis was the deadliest infectious agent before covid-19; 1.5 million deaths in 2020. Despite, a variety, of easy and cheap diagnostic tools, detection rates still fall below 90%; diagnosis delays are long exceeding 30 days many continents. This study aimed to determine risk factors for pulmonary TB Mali.
The aim of our study was to determine the frequency extended-spectrum beta-lactamase (ESBL) phenotypes among enterobacteria present in blood cultures patients at admission two university hospitals Bamako (Mali). During a period three months, we isolated from upon Point G and Gabriel Toure University Hospitals. ESBL-positive were initially identified by API 20E strips VITEK®2 then confirmed France MALDI-TOF mass spectrometry Bichat Hospital bacteriology laboratory. Antibiotic susceptibility...
Abstract Background Non-tuberculosis mycobacteria (NTMs) are environmental agents that can cause opportunistic pulmonary disease in humans and animals which is often misdiagnosed as tuberculosis (TB). In this study, we describe the cases of NTMs identified during first national anti-TB drug-resistance survey conducted Mali, associated risk factors. Methods Sputum was collected from people presenting for TB diagnosis, April to December 2019, regardless age. Microscopy-positive patients were...
Background Non-tuberculous mycobacteria (NTM) are environmental agents that can cause opportunistic pulmonary disease in humans and animals, often misdiagnosed as tuberculosis (TB). In this study, we describe the cases of NTM identified during first national anti-TB drug resistance survey conducted Mali explore associated risk factors. Methods Sputum was collected from people presenting for TB diagnosis April to December 2019, regardless age. Microscopy-positive patients were enrolled tested...
Introduction: While real-time reverse transcription PCR (RT-PCR) is the recommended laboratory method to diagnose severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2) infection, its use in resource limited settings can be difficult maintain due high testing demand and shortage of reagents. The aim this study was evaluate performances Realy Tech™ Standard Q™ comparison RT-PCR a relatively low COVID-19 prevalence setting, Mali.Methods: We conducted cross-sectional between January April...