A5100618410- Spinal Cord Injury Research
- Nerve injury and regeneration
- Cancer-related molecular mechanisms research
- Neurogenesis and neuroplasticity mechanisms
- Bone Metabolism and Diseases
- Bone Tissue Engineering Materials
- Spondyloarthritis Studies and Treatments
- TGF-β signaling in diseases
- Periodontal Regeneration and Treatments
- Dental Implant Techniques and Outcomes
- Mesenchymal stem cell research
- Spine and Intervertebral Disc Pathology
- Respiratory Support and Mechanisms
- MicroRNA in disease regulation
- Pain Mechanisms and Treatments
- Neonatal Respiratory Health Research
- Connective tissue disorders research
- Neuroinflammation and Neurodegeneration Mechanisms
- Orthopaedic implants and arthroplasty
- Angiogenesis and VEGF in Cancer
- Meningioma and schwannoma management
- Dental Radiography and Imaging
- Inhalation and Respiratory Drug Delivery
- IL-33, ST2, and ILC Pathways
- Kruppel-like factors research
Sun Yat-sen University
2015-2025
Key Laboratory of Guangdong Province
2020-2025
The First Affiliated Hospital, Sun Yat-sen University
2015-2025
Second Affiliated Hospital of Soochow University
2024
Soochow University
2016-2024
First Affiliated Hospital of Soochow University
2016-2024
Beijing Tongren Hospital
2023
Capital Medical University
2023
Shanghai Jiao Tong University
2021
State Key Laboratory of Mechanical System and Vibration
2021
Abstract Aged bone marrow mesenchymal stem cells (BMSCs) exhibit aberrant self-renewal and lineage specification, which contribute to imbalanced bone-fat progressive loss. In addition known master regulators of commitment, it is crucial identify pivotal switches governing the specific differentiation fate aged BMSCs. Here, we profiled differences in epigenetic regulation between adipogenesis osteogenesis identified super-enhancer associated lncRNA nuclear-enriched abundant transcript 1...
Osteoporosis is one of the most prevalent skeletal system diseases. It characterized by a decrease in bone mass and microarchitectural changes tissue that lead to an attenuation resistance susceptibility fracture. Vertebral fracture far osteoporotic In musculoskeletal system, osteoblasts, originated from marrow stromal cells (BMSC), are responsible for osteoid synthesis mineralization. osteoporosis, BMSC osteogenic differentiation defective. However, date, what leads defective osteogenesis...
To investigate the molecular mechanism underlying inflammation-related ectopic new bone formation in ankylosing spondylitis (AS).Spinal tissues and sera were collected from patients with AS healthy volunteers examined for expression of Wnt proteins. An vitro cell culture system mimicking local inflammatory microenvironment bone-forming sites was established to study relationship between inflammation expression, regulatory inflammation-induced role signaling formation. Modified...
Diabetic retinopathy (DR) is a serious microvascular complication of diabetes. This study demonstrates the antiangiogenic effects scutellarin (SCU) on high glucose- and hypoxia-stimulated human retinal endothelial cells (HRECs) diabetic rat model by oral administration. The mechanisms SCU in vitro vivo were investigated. HRECs cultured (30 mM D-glucose) hypoxia (cobalt chloride-treated)-stimulated condition to evaluate CCK-8 test, cell migration experiment (wound healing transwell), tube...
Ankylosing spondylitis (AS) is a chronic inflammatory disease characterized by back pain and spinal ankylosis due to pathological new bone formation. Here, we identified CXCL12 as critical contributor formation through recruitment of osteogenic precursor cells (OPCs). was found highly expressed in the regions that would potentially develop bone. OPCs were recruited where up-regulated. Inhibition CXCL12/CXCR4 axis with AMD3100 or conditional knockout CXCR4 attenuated migration subsequent...
Abstract Maxillary sinus membrane (MSM) elevation is a common surgical technique for increasing bone height in the posterior maxilla prior to dental implant placement. However, biological nature of regeneration MSM remains largely unidentified. In this study, tissue was obtained from 16 individuals during orthognathic surgery and used isolate stem cells (MSMSCs) by single-colony selection STRO-1 cell sorting. The characteristics terms colony-forming ability, surface antigens,...
Abstract Spinal cord injury (SCI) can lead to severe motor and sensory dysfunction, yet there are no effective therapies currently due the failure of reconstructing interruption neuroanatomical circuit. While neural stem cell (NSC) transplantation has been considered a potential strategy repair circuit after SCI, efficacy this remains unproven. The main reason is that most transplanted NSC differentiates into astrocyte rather than neuron in microenvironment SCI. Our results demonstrated Wnt4...
The aim of this study was to identify the role Piezo1-mediated mechanotransduction in entheseal pathological new bone formation and explore underlying molecular mechanism.Spinal ligament tissues were collected from 14 patients with ankylosing spondylitis (AS) non-AS controls bulk RNA sequencing conducted. Collagen antibody-induced arthritis models established observe formation. Pharmacological inhibition genetic ablation Piezo1 performed animal essential Piezo1. Entheseal osteo-chondral...
JOURNAL/nrgr/04.03/01300535-202502000-00032/figure1/v/2024-05-28T214302Z/r/image-tiff Invasive inflammation and excessive scar formation are the main reasons for difficulty in repairing nervous tissue after spinal cord injury. Microglia astrocytes play key roles injury micro-environment share a close interaction. However, mechanisms involved remain unclear. In this study, we found that injury, resting microglia (M0) were polarized into pro-inflammatory phenotypes (MG1 MG3), while reactive...
Article1 December 2020Open Access Source DataTransparent process Aberrant upregulation of CaSR promotes pathological new bone formation in ankylosing spondylitis Xiang Li Department Spine Surgery, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, China Guangdong Province Key Laboratory Orthopaedics and Traumatology, ChinaThese authors contributed equally to this work Search for more papers by author Siwen Chen Zaiying Hu Rheumatology Immunology, Sixth Dongying Jianru Wang...
Patients with osteoporosis have a high risk of implant loosening due to poor osteointegration, possibly leading failure, revision, and refracture. RNA interference therapy is an emerging epigenetic treatment, we found that miR-20a could enhance osteogenesis. Moreover, small extracellular vesicles (sEVs) derived from bone marrow mesenchymal stem cells (hBM-MSCs) were utilized as nanoscale carriers for the protection transportation (sEV-20a). In this study, intended determine whether sEVs...
Efficient osteogenetic differentiation and bone formation from muscle-derived stem cells (MDSCs) should have potential clinical applications in treating nonunion fracture healing or defects. Here, we investigate ability of MDSCs induced by morphogenetic protein 9 (BMP9) vitro rabbit radius defects repairing model. Rabbit's were extracted type I collagenase trypsin methods, BMP9 was introduced into infection with recombinant adenovirus. Effects BMP9-induced identified alkaline phosphatase...
Abstract Neural stem cells (NSCs) transplantation represents a promising strategy for the repair of injured neurons, since NSCs not only produce multiple neurotrophic growth factors but also differentiate into mature to replace damaged cells. Previous studies have shown that Notch signaling pathway had negative effects on neuronal differentiation; however, precise mechanism remained inadequately understood. This research aimed investigate whether inhibition Notch1 promotes differentiation...
We recently reported that maxillary sinus membrane stem cells (MSMSCs) have osteogenic potential. However, the biological mechanisms of bone formation remain unclear. In this study, we investigated role and microRNAs (miRNAs) in differentiation MSMSCs. The expression miRNAs was determined differentiated MSMSCs by comprehensive miRNA microarray analysis quantitative RT-PCR (qRT-PCR). selected miR-1827 for functional follow-up studies to explore its significance Here, found be up-regulated...
This study evaluated the expression pattern of micro RNAs (miRNAs) on a surface with nanotopography compared smooth (control).Human mesenchymal stem cells (hMSCs) were plated different surfaces and at 3, 7, 14 days for alkaline phosphatase (ALP) activity, genes (osterix [OSX], runt-related transcription factor 2 [RUNX2], bone morphogenetic protein [BMP2], ALP), miRNAs. Western blot was also used to detect osteogenic proteins (BMP2, OSX, osteocalcin [OCN]). Scanning electron microscopy onto...
Growing evidence shows that the inhibitory effect of inflammatory cytokines on new bone formation by osteogenic precursor cells is a critical cause net bone-density reduction. Melatonin has been proven to be potential therapeutic candidate for osteoporosis. However, whether it capable antagonizing suppressing so far elusive. In this study, using cell culture system human marrow stromal and MC3T3-E1 preosteoblasts, we recorded following vital observations provided insights melatonin-induced...
Traditional therapeutic strategies for spinal cord injury (SCI) are insufficient to repair locomotor function because of the failure axonal reconnection and neuronal regeneration in injured central nervous system (CNS). Neural stem cell (NSC) transplantation has been considered a potential strategy is generally feasible repairing neural circuit after SCI; however, most formidable problem that differentiation rate NSCs quite limited. Therefore, it essential induce improve repair. Our results...
Abstract Due to the various presentations of gastrointestinal tract duplications (GTD), diagnosing and management for this disease might be varied difficult. We intend improve experiences these difficult, in terms clinical presentations, diagnostic investigations, management. reviewed recent literature retrospectively analyzed 72 pediatric patients with enteric duplication. Diagnosis was confirmed by surgery pathological examination imaging characteristics features. The ages ranged from one...
Helicobacter pylori infection and alcohol intake are independent risk factors in gastric carcinogenesis; however, until now, the combined effect of H. consumption specific mechanism is still problematic. Here, we developed a series mouse models that progress from chronic gastritis to cancer, induced by infecting with then determining molecular progression flow cytometry, western blotting, qPCR, Mito Traker assay cancer T-cell lines. Interleukin-10 (IL-10) knockout mice was used determine...
Peripheral arterial disease (PAD) is a prevalent cardiovascular condition that affects up to 200 million people globally, and has significant implications for morbidity mortality. Recent studies have identified the red cell distribution width-to-albumin ratio (RDW/ALB) as potential systemic inflammatory marker correlated with several diseases including PAD. However, comprehensive role of RDW/ALB in PAD remains underexplored. The objective this study was elucidate relationship between provide...
Abstract Pathological new bone formation is the main cause of disability in ankylosing spondylitis (AS), and so far, it lacks a targeted therapy. Macrophages are central orchestrators inflammation progression tissue remodeling, but their contribution to pathological has largely not been explored. Here, identified that TREM2 + macrophages predominated within sites adjacent osteogenic precursor cells. In vivo, both depletion knockout Trem2 significantly reduced collagen antibody‐induced...