A5017516684- Mesenchymal stem cell research
- Cellular Mechanics and Interactions
- Cell Adhesion Molecules Research
- Nuclear Structure and Function
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
- Periodontal Regeneration and Treatments
- Bone Tissue Engineering Materials
- Muscle Physiology and Disorders
- Cardiac Valve Diseases and Treatments
- Tendon Structure and Treatment
- Shoulder Injury and Treatment
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Cancer Cells and Metastasis
- Anatomy and Medical Technology
- Surgical Simulation and Training
- Single-cell and spatial transcriptomics
- Microtubule and mitosis dynamics
- Shoulder and Clavicle Injuries
- Genetic and Kidney Cyst Diseases
- Coronary Artery Anomalies
- Orthopedic Surgery and Rehabilitation
- Phagocytosis and Immune Regulation
- Soft Robotics and Applications
Southern Medical University
2012-2024
Affiliated Hospital of Nantong University
2024
Nantong University
2024
University of Oklahoma Health Sciences Center
2024
Affiliated Hospital of Zunyi Medical College
2018
Seoul National University
2010
Artificial Intelligence in Medicine (Canada)
1994
United States National Library of Medicine
1994
Shizuoka Children's Hospital
1990
Abstract The mechanisms underlying the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) remain unclear. In present study, we aimed to identify key biological processes during differentiation. To this end, downloaded three microarray data sets from Gene Expression Omnibus (GEO) database: GSE12266, GSE18043 and GSE37558. Differentially expressed genes (DEGs) were screened using limma package, enrichment analysis was performed. Protein‐protein interaction network...
Abstract Background Actin is an essential cellular protein that assembles into microfilaments and regulates numerous processes such as cell migration, maintenance of shape, material transport. Methods In this study, we explored the effect actin polymerization state on osteogenic differentiation human adipose-derived stem cells (hASCs). The hASCs were treated for 7 days with different concentrations (0, 1, 5, 10, 20, 50 nM) jasplakinolide (JAS), a reagent directly polymerizes F-actin. effects...
The anterior talofibular ligament (ATFL) and calcaneofibular (CFL) contribute greatly to the overall stability of ankle joint; however, ATFL combined ATFL-CFL sprains are common. Anatomic reconstruction lateral collateral with grafts has been proposed for patients poor tissue quality or inadequate local tissue. ligaments requires a good understanding their anatomic location.To describe anatomy CFL quantitatively qualitatively explore relationship some morphological parameters.Descriptive...
Adipose-derived stem cells (ASCs) that show multidifferentiation and anti-immune rejection capacities have been widely used in plastic reconstructive surgery. Previous studies indicated mechanical biophysical interactions between their surrounding environment regulate essential processes, such as growth, survival, differentiation, the cytoskeleton system plays an important role mechanotransduction. However, of force determination lineage fate is still unclear. Human ASCs (hASCs) were...
Microenvironmental factors can modulate the cellular status of adipose tissue-derived stem cells (ASCs). In response to microenvironmental changes, remodel extracellular matrix (ECM) proteins, which play an important role in regulating cell behaviors. During adipogenic differentiation, ECM components secreted from ASCs dramatically. To evaluate stepwise adipogenesis-induced secretion on behavior ASCs, we cultured growth and media, was characterized decellularized. The were then reseeded...
Abstract Background The physical factors of the extracellular matrix have a profound influence on differentiation behavior mesenchymal stem cells. In this study, effect biophysical microenvironment rat bone marrow cell (BMSC) osteogenesis was studied both in vitro and vivo. Methods To prepare culture scaffolds varying stiffness, increasing amounts hydroxyapatite (HAp) were mixed into polyethylene glycol/silk fibroin (PEG/SF) solution. amount HAp ranged from 25 to 100 mg, which provided for...
Abstract Background Integrins play a prominent role in osteogenic differentiation by transmitting both mechanical and chemical signals. Integrin expression is closely associated with tensile stress, which has positive effect on differentiation. We investigated the relationship between integrin αVβ3 stress. Methods Human fibroblasts were treated c (RGDyk) lentivirus transduction to inhibit function of αVβ3. Y-15, cytochalasin D verteporfin used phosphorylation FAK, polymerization...
Tetraspanin CD151 is highly expressed in endothelia and reinforces cell adhesion, but its role vascular inflammation remains largely unknown.
The aim of this study was to investigate effects adipose-derived mesenchymal stem cells (AMSCs) on radioresistance breast cancer cells.MTT assays were used detect any influence AMSC supernatants proliferation cells; cell migration determine the effect recruitment AMSCs; survival fraction post-irradiation assessed by clonogenic assay; γ-H2AX foci number determined via fluorescence microscopy; and expression IGF-1R detected Western blotting.AMSC promoted cells. Breast could recruit AMSCs,...
Osteosarcoma, the most common primary bone tumor, occurs frequently in children and adolescents has a 5-year survival rate, which is unsatisfactory. As epidermal growth factor receptor (EGFR) positively correlates with TNM (tumor-node-metastasis) stage osteosarcoma, EGFR may play an important role its progression. The purpose of this study was to explore potential mechanisms underlying correlation. We found that EGF promotes MG63 cell migration invasion as well stress fiber formation via Rho...
Abstract Mesenchymal stem cells (MSCs) are known for their multilineage differentiation potential with immune-modulatory properties. The molecular underpinnings of remain largely undefined. In this study, we investigated the cellular and features chemically induced osteogenesis from MSC isolated human adipose tissue (human MSCs, hAMSCs) using single-cell RNA-sequencing (scRNA-seq). We found that a near complete osteogenic clusters hAMSCs under directional induction. Both groups...
Integrins are closely related to mechanical conduction and play a crucial role in the osteogenesis of human mesenchymal stem cells. Here we wondered whether tensile stress could influence cell differentiation through integrin αVβ3.We inhibited function αVβ3 cells by treating with c(RGDyk). Using cytochalasin D verteporfin inhibit polymerization microfilament nuclear Yes-associated protein (YAP), respectively. For each application, were loaded cyclic 10% at 0.5 Hz for 2 h daily. Mesenchymal...
A virus is arguably alive, and not a cell, but it depends on infecting cell in order to reproduce.†May also notes that possible half the extant species planet may become extinct next 50 100 years. HUNTER 15 18 ARTIFICIAL INTELLIGENCE & MOLECULAR BIOLOGY*kcal an abbreviation for kilocalorie, amount of energy necessary raise liter water one degree centigrade at standard temperature pressure.It equivalent 1 dieter's calorie.A mole substance, measured terms number molecules, rather than by its...
Human adipose-derived stem cells (hASCs) are ideal seed for tissue engineering due to their multidirectional differentiation potential. Microfilaments, microtubules, and intermediate filaments responsible supporting the intracellular space. Vimentin, a type III filament protein that is specifically expressed in of mesenchymal origin, can function as scaffold endow with tension shear stress resistance. Actin fibers (ASF) act an important physical device signal transduction, providing...
Primo-vessels have been observed in the rat abdominal cavity as floating thread like structures on and not adhering to fascia-wrapped internal organs. To date their presence, locations, lengths irregular unpredictable, identification regularly repeatable, thus they remained a nagging enigma primo-vascular system research for several years. In this work, locations were found where primo-vessels present repeatedly. These vessels or freely movable but lay regular position mesentery of rat,...
Human skin fibroblasts (HSFs) approximate the multidirectional differentiation potential of mesenchymal stem cells, so they are often used in differentiation, cell cultures, and injury repair. They an important seed source field bone tissue engineering. However, there a few studies describing mechanism osteogenic HSFs. Here, induction medium was to induce differentiate into osteoblasts, role mechanical sensitive element PDLIM5 microfilament-mediated human evaluated. The depolymerization...
Abstract The angle and position of the scapular glenoid are important in shoulder mechanics, interpretation diseases, planning replacement surgery. In total replacement, understanding bony parameters is also considerable guiding significance for designing implant size improving material adaptability. To compare measured from skeletal scapula specimens with those by 3D modeling CT scanning images, analyze correlations between these data, draw conclusions to guide clinical treatment joint...