A5100688618- MicroRNA in disease regulation
- Gut microbiota and health
- Circular RNAs in diseases
- Primate Behavior and Ecology
- Clostridium difficile and Clostridium perfringens research
- RNA modifications and cancer
- Animal Vocal Communication and Behavior
- Diet and metabolism studies
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Genetic Mapping and Diversity in Plants and Animals
- Nanopore and Nanochannel Transport Studies
- Mycorrhizal Fungi and Plant Interactions
- Cancer-related molecular mechanisms research
- Inflammatory Myopathies and Dermatomyositis
- Bacteriophages and microbial interactions
- Agriculture Sustainability and Environmental Impact
- Nuclear Receptors and Signaling
- Plant and Fungal Species Descriptions
- Animal Virus Infections Studies
- Plant and animal studies
- Animal Disease Management and Epidemiology
- Animal Behavior and Reproduction
- Cancer Treatment and Pharmacology
- Viral gastroenteritis research and epidemiology
Ministry of Industry and Information Technology
2025
Beihang University
2025
Chinese Academy of Sciences
1984-2025
Agro-Environmental Protection Institute
2024-2025
Shandong Tumor Hospital
2025
Shandong First Medical University
2025
Ministry of Agriculture and Rural Affairs
2024-2025
Women's Hospital, School of Medicine, Zhejiang University
2024
Peking University
2013-2024
Jinan University
2023-2024
Abstract The mechanisms underlying glucocorticoid (GC)-induced obesity are poorly understood. Macrophages the primary targets by which GCs exert pharmacological effects and perform critical functions in adipose tissue homeostasis. Here, we show that macrophages essential for GC-induced obesity. Dexamethasone (Dex) strongly induced Krüppel-like factor 9 ( Klf9 ) expression macrophages. Similar to Dex, lentivirus-mediated overexpression inhibits M1 M2a markers expression, causing macrophage...
To investigate why the level of Lyn is significantly decreased in B cells from a majority patients with systemic lupus erythematosus (SLE) and to determine role microRNA-30a (miR-30a) SLE cell hyperactivity.Luciferase reporter gene assays were performed identify interaction between miR-30a 3'-untranslated region (3'-UTR) Lyn. Levels determined by TaqMan quantitative polymerase chain reaction (qPCR), messenger RNA levels tested real-time qPCR, protein using Western blotting. The quantity IgG...
Weaning is one of the most stressful challenges in pig's life, which contributes to dysfunctions intestinal and immune system, disrupts gut microbial ecosystem, therefore compromises growth performance health piglets. To mitigate negative impact stress on early-weaned piglets, effective measures are needed promote health. Toward this end, we tamed a Saccharomyces cerevisiae strain developed probiotic Duan-Nai-An, yeast culture S. egg white. In study, tested Duan-Nai-An piglets analyzed its...
Background Circular RNAs (circRNAs) have been demonstrated to act as key regulators in the chemoresistance of human cancers, including breast cancer (BC). Here, we aimed explore role circ‐RNF111 paclitaxel (PTX) resistance BC. Methods Quantitative real‐time polymerase chain reaction (qRT‐PCR) was employed determine expression circ‐RNF111, microRNA‐140‐5p (miR‐140‐5p) and E2F transcription factor 3 (E2F3) mRNA. The half maximal inhibitory concentration (IC 50 ) PTX, cell viability, colony...
Abstract Grasslands provide a number of ecosystem services for human society. Degradation grasslands results in the loss biodiversity and leads to deterioration functions. In order accurately assess influence grassland degradation on belowground ecosystems, we conducted experiments temperate steppe with different levels investigated soil quality biotic communities. Our showed that significantly decreased quality, lower values index (SQI) observed degraded than meadow from forest-steppe...
Glioblastoma (GBM) is the most aggressive brain tumor. Reportedly, circular RNAs (circRNAs) participate in regulation of development and progression diverse cancers, including GBM.Dysregulated circRNAs GBM tissues were screened out from GEO database. The expression candidate cells was measured by qRT-PCR. Loss-of function assays, colony formation assay, EdU TUNEL flow cytometry analysis conducted to determine effects circ-AHCY knockdown on cell proliferation apoptosis. Animal study further...
Circular RNAs (circRNAs) play a crucial role in hepatocellular carcinoma (HCC) progression. However, the of exosomal circRNAs HCC is still largely unknown. We aimed to explore function circ-ZNF652 HCC.The morphology and size exosomes were examined by transmission electron microscopy (TEM) nanoparticle tracking analysis (NTA). The expression circ-ZNF652, ZNF652 mRNA, microRNA-29a-3p (miR-29a-3p) guanylyl cyclase domain containing 1 (GUCD1) mRNA was determined quantitative real-time polymerase...
Circular RNAs (circRNAs) function as essential regulators in diverse human cancers, including hepatocellular carcinoma (HCC). However, the of circ_0000517 HCC was unknown. We aimed to explore roles and mechanisms HCC.The levels circ_0000517, RPPH1 mRNA microRNA-1296-5p (miR-1296-5p) were measured using quantitative real-time polymerase chain reaction (qRT-PCR). The characteristics explored by RNase R digestion actinomycin D assays. Cell proliferation evaluated Counting Kit-8 (CCK-8) colony...
The changes in dietary patterns cause an increased incidence of colorectal cancer (CRC) globally. We aimed to explore the mechanism behind circular RNA circ_0136666 progression CRC.The expression circ_0136666, miR-383 and cAMP response element binding protein 1 (CREB1) was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Cell proliferation, apoptosis glycolysis were measured 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), flow cytometry glucose or...
To investigate the influence of group counselling on career planning and maturity male nursing students.Sixty students were randomly selected from a specific-level first-class hospital in Hunan Province July to August 2020 by using convenience sampling method subsequently divided into control experimental random number table method. The received routine pre-job training, including aspects concerning profile, nurse etiquette, core systems, professional ethics, emergency treatment prospects...
Soil addition is now widely used in the restoration of degraded ecosystems, but how soil influences multiple ecological functions grasslands, and whether these effects depend on amount type inoculum, are still not clear. We performed two parallel experiments to examine different donor types amounts affect grassland. In a field experiment at grassland site where top layer was removed (5 cm), we assessed effect collected from ecosystems (upland meadow steppe) (0 cm, 1 cm 3 cm) ecosystem...
Radioactive 131I therapy is a primary treatment for papillary thyroid carcinoma (PTC), with approximately 30% of patients developing iodine-refractory disease. There an urgent clinical need to improve iodine uptake in PTC. Previous research suggested connection between triglyceride (TG) accumulation and decreased benign cells. Notably, TG has been found be marker aggressive human Therefore, it crucial elucidate whether affects PTC, which may lead new way enhancement uptake. Here, by...
The gut microbiota plays an important role in animal health and is strongly affected by the environment. Captivity human source food have been shown to influence drastically composition function of wild animals. Therefore, present study, provisioned populations limestone-living rhesus macaques (Macaca mulatta) were compared using high-throughput 16S rRNA sequencing bioinformatic analyses. results indicated that had a higher microbial richness than macaques, but there was no significant...
The microbiota has been observed altered in autoimmune diseases, including idiopathic inflammatory myopathies (IIMs), and associated with different treatments. Low-dose IL-2 treatment emerges as a new option for active IIMs. This study aims to explore the role of low-dose regulating intestinal dysbiosis involved In this study, 13 patients IIMs were enrolled received 1 ×10 6 IU subcutaneously every other day 12 weeks plus standard care. clinical response immune assessed. Stool samples...
Gramicidin A (gA) channels provide an ideal system to test molecular dynamics (MD) simulations of membrane proteins. The peptide backbone lines a cation-selective pore, and due the small channel size, average structure extent fluctuations all atoms in will influence ion permeation. This raises question how well mechanical force fields used MD potential mean (PMF) calculations can predict as To address this question, we undertook comparative study nuclear magnetic resonance (NMR) observables...