A5086695733- Wound Healing and Treatments
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Hip and Femur Fractures
- Diabetic Foot Ulcer Assessment and Management
- Mesenchymal stem cell research
- Circular RNAs in diseases
- Bone Metabolism and Diseases
- Electrospun Nanofibers in Biomedical Applications
- Immune cells in cancer
- Orthopaedic implants and arthroplasty
- Shoulder Injury and Treatment
- Hip disorders and treatments
- Bone fractures and treatments
- COVID-19 and healthcare impacts
- Spinal Fractures and Fixation Techniques
- Bone and Joint Diseases
- Periodontal Regeneration and Treatments
- RNA modifications and cancer
- Trauma Management and Diagnosis
- Graphene and Nanomaterials Applications
- Pelvic and Acetabular Injuries
- Orthopedic Surgery and Rehabilitation
- COVID-19 Clinical Research Studies
- Cancer-related molecular mechanisms research
Huazhong University of Science and Technology
2016-2025
Hubei Zhongshan Hospital
2021-2025
Wuhan University
2020-2025
Union Hospital
2016-2025
Union Hospital
2009-2024
Wuhan Union Hospital
2020-2021
Huazhong University of Science and Technology Hospital
2020
Abstract The treatment of diabetic wounds remains a major challenge in clinical practice, with chronic characterized by multiple drug‐resistant bacterial infections, angiopathy, and oxidative damage to the microenvironment. Herein, novel situ injectable HA@MnO 2 /FGF‐2/Exos hydrogel is introduced for improving wound healing. Through simple local injection, this able form protective barrier covering wound, providing rapid hemostasis long‐term antibacterial protection. MnO /ε‐PL nanosheet...
Diabetic wound (DW) therapy is currently a big challenge in medicine and strategies to enhance neurogenesis angiogenesis have appeared be promising direction. However, the current treatments failed coordinate simultaneously, leading an increased disability rate caused by DWs. Herein, whole-course-repair system introduced hydrogel concurrently achieve mutually supportive cycle of neurogenesis-angiogenesis under favorable immune-microenvironment. This can first one-step packaged syringe for...
The osteoblast/osteoclast and M1/M2 macrophage ratios play critical roles in delayed fracture healing. Robust osteoblast differentiation M2 polarization can substantiality promote repair; however, the combined effect of these strategies has not been previously studied. In this study, we constructed a cocktail therapy to simultaneously regulate balance. composed natural polymer hyaluronic-acid-based hydrogel (HA hydrogel, which tissue-adhesive, injectable, self-healing, anti-inflammation...
Abstract Background Osteoblast differentiation is a vital process for fracture healing, and exosomes are nanosized membrane vesicles that can deliver therapeutic drugs easily safely. Macrophages participate in the regulation of various biological processes vivo, macrophage-derived (MD-Exos) have recently been topic increasing research interest. However, few study has explored link between MD-Exos osteoblast differentiation. Herein, we sought to identify miRNAs differentially expressed M1 M2...
Diabetic wounds remain a great challenge for clinicians due to the multiple bacterial infections and oxidative damage. Exosomes, as an appealing nanodrug delivery system, have been widely applied in treatment of diabetic wounds. Endovascular cells are important component vascular wall. Herein, we investigated effects HUCMSCs HUC-Exos (exosomes secreted by HUCMSCs) on wound healing. In this study, HUVECs were coincubated with HUCMSCs, utilized vitro vivo experiments verify their roles...
MicroRNAs (miRNAs) broadly regulate normal biological functions of bone and the progression fracture healing osteoporosis. Recently, it has been reported that miR-1224-5p in plasma is a potential therapy for osteogenesis. To investigate roles Rap1 signaling pathway osteoporosis development progression, we used BMMs, BMSCs, skull osteoblast precursor cells vitro osteogenesis osteoclastogenesis studies. Osteoblastogenesis were detected by ALP, ARS, TRAP staining slice resorption pit assays....
The diabetic wounds remain to be unsettled clinically, with chronic characterized by drug-resistant bacterial infections, compromised angiogenesis and oxidative damage the microenvironment. To ameliorate stress applying antioxidant treatment in wound site, we explore function of folliculin-interacting protein 1 (FNIP1), a mitochondrial gatekeeper works alter morphology, reduce phosphorylation protect cells from unwarranted ROS accumulation. And our vitro experiments showed effects FNIP1...
The diabetic wounds remain to be unsettled clinically, with chronic characterized by drug-resistant bacterial infections, compromised angiogenesis and oxidative damage the microenvironment. Hence, a novel designed glucose-responsive HA-PBA-FA/EN106 hydrogel is introduced for improving wound healing. Due dynamic phenylboronate ester structure phenylboronic acid group between hyaluronic (HA) (PBA), able realize release of drugs. Fulvic (FA) added in hydrogel, which not only severs as...
Oxidative stress, infection, and vasculopathy caused by hyperglycemia are the main barriers for rapid repair of foot ulcers in patients with diabetes mellitus (DM). In recent times, discovery neddylation, a new type post-translational modification, has been found to regulate various crucial biological processes including cell metabolism cycle. Nevertheless, its capacity control healing wounds diabetic remains unknown. This study shows that MLN49224, compound inhibits neddylation at low...
Bone nonunion poses an urgent clinical challenge that needs to be addressed. Recent studies have revealed the metabolic microenvironment plays a vital role in fracture healing. Macrophages and bone marrow-derived mesenchymal stromal cells (BMSCs) are important targets for therapeutic interventions fractures. Itaconate is TCA cycle metabolite has emerged as potent macrophage immunomodulator limits inflammatory response. During osteogenic differentiation, BMSCs tend undergo aerobic glycolysis...
Abstract Background Enhancing angiogenesis is critical for accelerating wound healing. Application of different types exosomes (Exos) to promote represents a novel strategy enhanced repair. Saliva known accelerate healing, but the underlying mechanisms remain unclear. Results Our results have demonstrated that saliva-derived (saliva-Exos) induce human umbilical vein endothelial cells (HUVEC) proliferation, migration, and in vitro, cutaneous healing vivo. Further experiments documented...
Diabetic foot ulcers are a common complication of diabetes, and usually incurable in the clinic.Exosomes (carriers that transfer endogenous molecules) from diabetic patients' blood have been demonstrated to suppress wound repair.In this study, we investigated effects circulating exosomal microRNA-15a-3p (miR-15a-3p) on repair.Exosomes were extracted blood, found inhibit repair vitro vivo.miR-15a-3p was upregulated exosomes, impaired healing.When miR-15a-3p knocked down their negative...
A moderate inflammatory response at the early stages of fracture healing is necessary for callus formation. Over-active and continuous inflammation, however, impairs leads to excessive tissue damage. Adequate could be promoted through suppression local over-active immune cells in site. In present study, we achieved an enriched concentration PD-L1 from exosomes (Exos) a genetically engineered Human Umbilical Vein Endothelial Cell (HUVECs), demonstrated that overexpressing specifically bind...
Abstract Fracture combined with traumatic brain injury (TBI) is one of the most common and serious types compound trauma in clinic characterized by dysfunction cellular communication injured organs. Our prior studies found that TBI was capable enhancing fracture healing a paracrine manner. Exosomes (Exos), as small extracellular vesicles, are important vehicles for noncell therapy. However, whether circulating Exos derived from patients (TBI-Exos) regulate prohealing effects fractures...
Abstract N6‐methyladenosine (m6A) modification has been reported in various diseases and implicated increasing numbers of biological processes. However, previous studies have not focused on the role m6A fracture healing. Here, we demonstrated that modifications are decreased during healing methyltransferase‐like 3 (METTL3) is main factor involved abnormal changes modifications. Down‐regulation METTL3 promotes osteogenic processes both vitro vivo, this effect recapitulated by suppression...
Patients who sustain a traumatic brain injury (TBI) are known to have significantly quicker fracture healing time than patients with isolated fractures, but the underlying mechanism has yet be identified. In this study, we found that upregulation of miRNA-26a-5p induced by TBI correlated decrease in phosphatase and tensin homolog (PTEN) callus formation. vitro, overexpressing inhibited PTEN expression accelerated osteoblast differentiation, whereas silencing activity. Reduction facilitated...
Fracture healing is a complex process involving various cell types, cytokines, and mRNAs. Here, we report the roles of circRNA AFF4/miR-7223-5p/PIK3R1 axis during fracture healing. We found that increased expression PIK3R1 directly associated with augmented proliferation decreased apoptosis MC3T3-E1 cells. Furthermore, miR-7223-5p targeted PI3KR1 inhibited while promoting apoptosis. CircRNA AFF4 acted as sponge miR-7223-5p, thereby inhibiting Local injection into femoral sites promoted in...
Hip fractures in the elderly account for more than half of osteoporotic and represent a substantial economic social burden. Novel coronavirus pneumonia (COVID-19), which began to spread December 2019, has created challenges management hip fracture patients, not only by influencing choice operation postoperative rehabilitation methods, but also generating new risks medical staff. During this period, our infection orthopedic treatment unit center epidemic area effectively treated 82 patients...
The angiotensin-converting enzyme 2 (ACE2) receptor has been identified as the cell entry point for SARS-CoV-2.Although ACE2 receptors are present in bone marrow, effects of SARS-CoV-2 on biological activity tissue have not yet elucidated.In study we sought to investigate impact osteoblastic context fracture healing.MicroRNA-4485 (miR-4485), which found be upregulated COVID-19 patients, negatively regulates osteogenic differentiation.We demonstrate this effect both vitro and vivo.Moreover,...
The immune system and skeletal are closely linked. Macrophages one of the most important cells for bone remodeling, playing a prohealing role mainly through M2 phenotype polarization. Baicalein (5,6,7-trihydroxyflavone, BCL) has been well documented to have noticeable promotion effect on macrophage However, due limitations in targeted delivery macrophages toxic other organs, BCL rarely used treatment fractures. In this study, we developed mesoporous silica Fe3O4 composite-targeted...
The review delves into exploring diverse cellular sources for coating nanoparticles to treat osteoporosis. Furthermore, it highlights the pivotal role of these in restoring immune microenvironment, offering promising avenues combating