A5046824769- Optical Coherence Tomography Applications
- Advanced Fluorescence Microscopy Techniques
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Photoacoustic and Ultrasonic Imaging
- Glaucoma and retinal disorders
- Genomics, phytochemicals, and oxidative stress
- RNA modifications and cancer
- Glutathione Transferases and Polymorphisms
- Corneal surgery and disorders
- Kruppel-like factors research
- Genetics and Neurodevelopmental Disorders
- DNA Repair Mechanisms
- Neurogenetic and Muscular Disorders Research
- Cancer-related gene regulation
- Nanoparticles: synthesis and applications
- Random lasers and scattering media
- Neonatal Respiratory Health Research
- Cancer-related molecular mechanisms research
- Genetic Neurodegenerative Diseases
- Environmental Toxicology and Ecotoxicology
- Nitric Oxide and Endothelin Effects
- Toxic Organic Pollutants Impact
- Coronary Interventions and Diagnostics
- Histone Deacetylase Inhibitors Research
University of Tsukuba
2016-2023
Japan Society for the Promotion of Science
2022-2023
Abstract LSD1/KDM1A is a widely conserved lysine-specific demethylase that removes methyl groups from methylated proteins, mainly histone H3. We previously isolated the zebrafish LSD1 gene and demonstrated it required for primitive hematopoiesis. Recently, neuron-specific splicing variant of was found in mammals its specific functions substrate specificities were reported. To our surprise, cDNA, which we analyzed, corresponded to mammals. In this study, investigated structures expression...
The Keap1–Nrf2 pathway is an evolutionarily conserved mechanism that protects cells from oxidative stress and electrophiles. Under homeostatic conditions, Keap1 interacts with Nrf2 leads to its rapid proteasomal degradation, but when are exposed stress/electrophiles, senses them, resulting in improper interaction stabilization. therefore considered both "inhibitor" of "stress sensor" for activation. Interestingly, fish amphibians have two Keap1s (Keap1a Keap1b), while there only one mammals,...
Cerebellar ataxia is a genetically heterogeneous disorder. GEMIN5 encoding an RNA-binding protein of the survival motor neuron complex, essential for small nuclear ribonucleoprotein biogenesis, and it was recently reported that biallelic loss-of-function variants cause neurodevelopmental delay, hypotonia, cerebellar ataxia. Here, whole-exome analysis revealed compound heterozygous in two individuals from our cohort 162 patients with atrophy/hypoplasia. Three novel truncating one previously...
The Keap1‐Nrf2 system is an evolutionarily conserved defense mechanism against oxidative and xenobiotic stress. Besides the exogenous stress response, Nrf2 has been found to regulate numerous cellular functions, including protein turnover glucose metabolism; however, evolutionary origins of these functions remain unknown. In present study, we searched for novel target genes associated with zebrafish answer this question. A microarray analysis embryos that overexpressed revealed 115 candidate...
The scattering and polarization characteristics of various organs in vivo wildtype zebrafish three development stages were investigated using a non-destructive label-free approach. presented results showed promising first step for the usability Jones-matrix optical coherence tomography (JM-OCT) zebrafish-based research.We aim to visualize quantify scatter signatures larvae, juvenile, young adult animals non-invasive way.A custom-built polarization-sensitive JM-OCT setup combination with...
The Keap1–Nrf2 pathway is an evolutionarily conserved mechanism that protects cells from oxidative stress and electrophiles. Keap1 a repressor of Nrf2 in normal cellular conditions but also sensor for activation. Interestingly, fish amphibians have two Keap1s (Keap1a Keap1b), which Keap1b the ortholog mammalian Keap1. Keap1a, on other hand, gene found only amphibians, having been lost during evolution to amniotes. We previously shown keap1b-knockout zebrafish increased activity reduced...
A new formulation of the lateral imaging process point-scanning optical coherence tomography (OCT) and a differential contrast method designed by using this are presented. The is based on mathematical sample model called dispersed scatterer (DSM), in which represented as material with spatially slowly varying refractive index randomly distributed scatterers embedded material. It shown that represents meaningful OCT image speckle two independent quantities. complex signal processing images,...
Multiple scattering is one of the main factors that limits penetration depth optical coherence tomography (OCT) in samples. We propose a method termed multi-focus averaging (MFA) to suppress multiple-scattering signals and improve image contrast OCT deep regions. The MFA captures multiple volumes with various focal positions averages them complex form after correcting varying defocus through computational refocusing. Because takes different trajectories among position configurations, this...
Antioxidant effects of soy-derived isoflavones are predicted to be mediated by the Keap1-Nrf2 pathway. Recently, we constructed an assay system evaluate antioxidant dietary phytochemicals in zebrafish and revealed a relationship between these In this study, used examine seven isoflavones. Among those seven, equol showed strong when arsenite was as oxidative stressor. The effect also shown Nrf2-mutant nfe2l2afh318, suggesting that not To elucidate unidentified mechanism, gene expression...
Polarization-sensitive optical coherence tomography (PS-OCT) is a promising biomedical imaging tool for the differentiation of various tissue properties. However, presence multiple-scattering (MS) signals can degrade quantitative polarization measurement accuracy. We demonstrate method to reduce MS and increase accuracy Jones matrix PS-OCT. This suppresses by averaging multiple volumes measured using different focal positions. The are decorrelated among focus position modulation thus reduced...
DNA polymerase epsilon (Pol ε), a component of the core replisome, is involved in replication. Although genetic defects Pol ε have been reported to cause immunodeficiency syndromes, its role haematopoiesis remains unknown. Here, we identified compound heterozygous variants (p.[Asp1131fs];[Thr1891del]) POLE , encoding catalytic subunit A (POLE1), siblings with syndromic form severe congenital transfusion-dependent anaemia. In contrast Diamond-Blackfan anaemia, marked reticulocytopenia or...
Abstract Breast cancer is a leading cause of death in female patients worldwide. Further research needed to get deeper insight into the mechanisms involved development this devastating disease and find new therapy strategies. The zebrafish an established animal model, especially field oncology, which has shown be promising candidate for pre-clinical precision-based medicine. To investigate growth vivo zebrafish, one approach explore xenograft tumor models. In article, we present...
Optical coherence tomography (OCT) has been utilized to investigate the optical properties of tissue in animal model studies. Polarization sensitive OCT (PS-OCT) is one functional extensions OCT, which provides additional contrasts such as birefringence and degree-of-polarization uniformity (DOPU). It enables multi-contrast imaging for discrimination biological samples. However, samples often exhibit multiple scattering (MS). severely hampers penetration depth degrades property measurement...
We reported the involvement of oxidative stress and prostaglandins including thromboxane prostacyclin in pre-cardiac edema (early edema) caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). While TCDD-induced toxicity has been frequently reported, mechanism its action is still unclear. In present study, inducers paraquat, hydrogen peroxide (H2O2) rotenone augmented early (edema) induced a low concentration TCDD (0.1 ppb) at 55 hr post fertilization (hpf), while each them alone did not cause...
We propose a new multi-focus average method for optical coherence tomography, to reduce the multiple scattering signals and improve visibility of sample structure in deep region. It combines computational refocusing, complex averaging, acquisitions with focal shifting. A phantom was measured validate suppression contrast improvement at
The zebrafish is a valuable animal model in pre-clinical cancer research. Optical coherence tomography (OCT) non-invasive optical imaging technique, which provides label-free and three-dimensional method to investigate the tissue structure. Jones-matrix OCT (JM-OCT) functional extension of conventional OCT, gain additional specific contrast by analyzing polarization states back-scattered light. In this work we present longitudinal investigation vivo wildtype tumor xenograft using our JM-OCT...
Differential interference microscopy (DIC) is a method to obtain the refractive index distribution of sample as contrast. It suitable for biological cells, however, DIC can only 2D images from thin samples. Therefore, we introduce new imaging method, volumetric differential contrast (VDC) using optical coherence tomography (OCT). This enables getting 3D thick VDC was designed based on disperse scatterer model (DSM), theoretical OCT, and obtains by complex numerical processing OCT signal. DSM...
The zebrafish has shown to be an essential preclinical animal model, especially in the field of oncology. A non-invasive, high-resolution, and three-dimensional imaging modality is required identify disease related changes this model organism. presented polarization-sensitive Jones matrix optical coherence tomography (JM-OCT) prototype was utilized three different studies, covering vivo wildtype zebrafish, adult postmortem tumor a longitudinal xenograft investigation. This work highlights...
A new formulation of lateral imaging process point-scanning optical coherence tomography (OCT) and a differential contrast method designed by using this are presented. The is based on mathematical sample model called the dispersed scatterer (DSM), in which represented as material with spatially slowly varying refractive index randomly distributed scatterers embedded material. It shown that represents meaningful OCT image speckle two independent quantities. complex signal processing images,...
Multiple scattering is one of the main factors that limits penetration depth optical coherence tomography (OCT) in samples. We propose a method termed multi-focus averaging (MFA) to suppress multiple-scattering signals and improve image contrast OCT deep regions. The MFA captures multiple volumes with various focal positions averages them complex form after correcting varying defocus through computational refocusing. Because takes different trajectories among position configurations, this...
Polarization-sensitive optical coherence tomography (PS-OCT) is a promising biomedical imaging tool for differentiation of various tissue properties. However, the presence multiple-scattering (MS) signals can degrade quantitative polarization measurement accuracy. We demonstrate method to reduce MS and increase accuracy Jones matrix PS-OCT. This suppresses by averaging multiple volumes measured using different focal positions. The are decorrelated among focus position modulation thus reduced...