A5078602747- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Heart Rate Variability and Autonomic Control
- Respiratory Support and Mechanisms
- Single-cell and spatial transcriptomics
- T-cell and B-cell Immunology
- Diabetes and associated disorders
- Axon Guidance and Neuronal Signaling
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- interferon and immune responses
- Parvovirus B19 Infection Studies
- Peripheral Neuropathies and Disorders
- Immune responses and vaccinations
- Intensive Care Unit Cognitive Disorders
- Ultrasonics and Acoustic Wave Propagation
- Immune Cell Function and Interaction
- Long-Term Effects of COVID-19
- Cardiovascular and exercise physiology
- Musculoskeletal pain and rehabilitation
- Inflammasome and immune disorders
- Porphyrin and Phthalocyanine Chemistry
- Bone Metabolism and Diseases
- Bone health and treatments
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
Osaka University
2000-2025
Kobe University
2025
Kumamoto University
2024
Osaka International University
2022-2024
Mochida Pharmaceutical (Japan)
2023
Kyushu University
2023
Kyoto University
2023
Yamaguchi University
1981-2019
Laboratoire de Biochimie
2000
Mechanisms underpinning the dysfunctional immune response in severe acute respiratory syndrome coronavirus 2 infection are elusive. We analyzed single-cell transcriptomes and T B cell receptors (BCR) of >895,000 peripheral blood mononuclear cells from 73 disease 2019 (COVID-19) patients 75 healthy controls Japanese ancestry with host genetic data. COVID-19 showed a low fraction nonclassical monocytes (ncMono). report downregulated transitions classical to ncMono reduced CXCL10 expression...
Consecutive mRNA vaccinations against SARS-CoV-2 reinforced both innate and adaptive immune responses. However, it remains unclear whether the enhanced responses are mediated by epigenetic regulation and, if so, these effects persist. Using mass cytometry, RNA-Seq, ATAC-Seq, we show that BNT162b2 vaccination upregulated antiviral IFN-stimulated gene expression in monocytes with greater after second than those first vaccination. Transcription factor-binding motif analysis also revealed...
We measured the changes in heart rate (HR) variability estimated from standard deviation of R-R intervals to evaluate cardiac parasympathetic tone noninvasively before and during activation muscle metaboreflex induced by postexercise ischemia. Eight healthy male subjects performed sustained handgrip at 50% maximal voluntary contraction followed forearm occlusion. Mean arterial pressure, stroke volume, ratio preejection period left ventricular ejection time (PEP/LVET) were also measured....
Sex-biased humoral immune responses to COVID-19 patients have been observed, but the cellular basis for this is not understood. Using single-cell proteomics by mass cytometry, we find disrupted regulation of immunity in patients, with a sex-biased loss circulating follicular regulatory T cells (cTfr) at significantly greater rate male patients. In addition, sex-associated network T-peripheral helper, plasma blasts, proliferating and extrafollicular/atypical CD11c+ memory B was strongly...
Immune checkpoint inhibitors (ICIs) are indicated for a diverse range of cancer types, and characterizing the tumor immune microenvironment is critical optimizing therapeutic strategies, including ICIs. T cell infiltration activation status in greatly affects efficacy Here, we show that semaphorin 6D (Sema6D) forward signaling, which reportedly involved coordinating orientation development migration as guidance factor, impaired tumor-specific CD8+ cells murine oral tumors. Sema6D expressed...
Regulated neural-metabolic-inflammatory responses are essential for maintaining physiological homeostasis. However, the molecular machinery that coordinates neural, metabolic, and inflammatory is largely unknown. Here, we show semaphorin 6D (SEMA6D) anxiogenic, outputs from amygdala by synaptic Using genome-wide approaches, identify SEMA6D as a pleiotropic gene both psychiatric metabolic traits in human. Sema6d deficiency increases anxiety mice. When fed high-fat diet, Sema6d−/− mice display...
Abstract Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection can lead to severe disease 2019 (COVID-19), which is characterized by cytokine storm and organ dysfunction. The spike S1 subunit induces inflammatory production, but the immune cell subsets that respond stimulation contribute severity remain unclear. Methods We analyzed serum samples peripheral blood mononuclear cells (PBMCs) from patients with COVID-19 (moderate: n = 7; severe: 25) healthy controls (...
Systemic lupus erythematosus (SLE) is a heterogeneous autoimmune disease, and many peripheral immune cell populations (ICPs) are thought to be altered according the course of disease. However, it unclear which ICPs associated with clinical phenotypes SLE. We analyzed blood mononuclear cells (PBMCs) 28 SLE patients using mass cytometry identified 30 ICPs. determined proliferative activity by measuring proportion expressing specific markers Ki-67 among CD45+ (Ki-67+ proportion). observed an...
Exacerbation of rheumatic disease after vaccination against SARS-CoV-2 is being reported. However, there are only a few cases new-onset diseases. We present two persistent polyarthritis that developed in patients receiving the mRNA vaccine SARS-CoV-2. One patient had bilateral pleural effusions with markedly elevated serum interferon (IFN)-β, while other no effusion, IFN-β comparable healthy subjects. Other cytokines were unaltered association effusion. Both responded well to treatment 20 mg...
Group 2 innate lymphoid cells (ILC2s) have been implicated in both physiologic tissue remodeling and allergic pathology, yet the niche signaling required for ILC2 properties is poorly understood. Here, we show that an axonal guidance cue semaphorin 6D (Sema6D) plays critical roles maintenance of IL-10–producing ILC2s. Sema6d −/− mice exhibit a severe steady-state reduction ILC2s peripheral sites such as lung, visceral adipose tissue, mesentery. Interestingly, loss Sema6D results suppressed...
To evaluate the efficacy and safety of escitalopram (ESC) in a 48-week relapse prevention study Japanese adolescents with major depressive disorder (MDD).
The coronavirus disease 2019 (COVID-19) pandemic is widespread; however, accurate predictors of refractory cases have not yet been established. Circulating extracellular vesicles, involved in many pathological processes, are ideal resources for biomarker exploration.To identify potential serum biomarkers and examine the proteins associated with pathogenesis COVID-19, we conducted high-coverage proteomics on vesicles collected from 12 patients COVID-19 at different severity levels 4 healthy...
TAPL is a half-type ABC transporter with sequence similarity to TAP1 and TAP2 that transcribed in various rat tissues [Yamaguchi, Y., Kasano, M., Terada, t, Sato, R., Maeda, M. (1999) FEBS Lett 457, 231-236]. Primary structures of the human mouse orthologous counterparts were deduced from cDNAs cloned by means polymerase chain reaction, they compared rat. The mammalian TAPLs (rat, mouse, human) are highly conserved, since about 95% amino acid residues identical between rodents man....
Autoimmune inflammatory rheumatic disease (AIRD) patients are at high risk of the coronavirus 2019 (COVID-19), but medium-term effects immunosuppressants on vaccine efficacy unknown. We investigated duration humoral responses against severe acute respiratory syndrome 2 (SARS-CoV-2) wild-type and Omicron variant in AIRD administered with two doses BNT162b2 (Pfizer-BioNTech) vaccine.Serum-neutralizing antibody (NAb) anti-receptor-binding domain (RBD)/spike levels were measured. Short- analyzed...
One of the oligo(p-phenylene vinylene) derivatives having alkyl chains, OPV-1, is known to form characteristic rod-shaped nanoaggregates on water surface, and thin films with aligned can be obtained by Langmuir–Blodgett (LB) technique. Although molecular aggregation orientation "conjugated rings part" in LB have already been investigated, analysis detailed structure involving "alkyl chains" left behind. In present study, reveal role chains Langmuir prior transfer, infrared (IR) external...
Abstract Using single-cell proteomics by mass cytometry, we investigate changes to a broad selection of over 10,000,000 immune cells in cohort moderate, severe, and critical Japanese COVID-19 patients healthy controls with particular focus on regulatory T-cells (Tregs). We find significant disruption within all compartments the system emergence atypical CTLA-4 high CD4 proliferating HLA-DR low CD38 Tregs associated patients. also observed disrupted regulation humoral immunity COVID-19, loss...
The theory of free-vibration acoustic resonance (FVAR) solids at high pressure is developed within the framework nonlinear elasticity and calculus variations. FVAR state formulated as a generalization conventional that was originally by Rayleigh Ritz in sense it includes geometrical material nonlinearities well potential energy external pressure. Magnetic point groups quasi-harmonic approximation are used so to obtain natural extension normal mode phonons high-pressure regime. numerical...
This study aimed to clarify whether infection by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is prevalent among the staff of a hospital providing treatment patients with disease 2019 (COVID-19) using radioligand assay (RLA). One thousand samples from general COVID-19 were assayed for SARS-CoV-2 nucleocapsid protein (N) IgG RLA. Nine who had been treated in inpatient settings and already recovered used as control subjects, 186 blood donor obtained more than 10 years ago...
Abstract Neuropathic pain often results from injuries and diseases that affect the somatosensory system. Disruption of circadian clock has been implicated in exacerbation neuropathic state. However, this study, we report mice deficient a core component Period2 (Per2m/m mice) fail to develop tactile hypersensitivity even following peripheral nerve injury. Similar male wild-type mice, partial sciatic ligation (PSL)-Per2m/m showed activation glial cells dorsal horn spinal cord increased...
A 25-year-old woman with a history of B-cell acute lymphoblastic leukemia over ten years ago was referred to our hospital chief complaint leukoblastosis. She participating in JPLSG (Japanese Pediatric Leukemia/Lymphoma Study Group) clinical study at that time. We diagnosed ALL relapse by multi-color flow cytometric analysis bone marrow samples admission, reference previous data. Because her leukemic cells were resistant conventional cytotoxic agents, she proceeded lymphocyte apheresis for...
Whereas severe COVID-19 is often associated with elevated autoantibody titers, the underlying mechanism behind their generation has remained unclear. Here we report clonal composition and diversity of autoantibodies in humoral response to SARS-CoV-2. Immunoglobulin repertoire analysis characterization plasmablast-derived monoclonal antibodies uncovered expansion plasmablasts producing cardiolipin (CL)-reactive autoantibodies. Half expanded CL-reactive clones exhibited strong binding...