A5101531986- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Osteoarthritis Treatment and Mechanisms
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Molecular Sensors and Ion Detection
- Silk-based biomaterials and applications
- Luminescence and Fluorescent Materials
- Hippo pathway signaling and YAP/TAZ
- RNA Research and Splicing
Sir Run Run Shaw Hospital
2017-2023
Zhejiang University
2017-2023
Blood Center of Zhejiang Province
2020-2023
Objectives Circular RNAs (circRNA) expression aberration has been identified in various human diseases. In this study, we investigated whether circRNAs could act as competing endogenous to regulate the pathological process of osteoarthritis (OA). Methods CircRNA deep sequencing was performed between OA and control cartilage tissues. The regulatory functional role CircSERPINE2 upregulation examined validated vitro vivo, downstream target explored. RNA pull down, a luciferase reporter assay,...
Osteoarthritis (OA) is a common joint disease characterized by progressive loss of cartilage and reduction in lubricating synovial fluid, which lacks effective treatments currently. Here, we propose hydrogel-based miRNA delivery strategy to rejuvenate impaired creating regenerative microenvironment mitigate chondrocyte senescence that mainly contributes breakdown during OA development. An aging-related miRNA, miR-29b-5p, was first found be markedly down-regulated cartilage, their...
CircMYO10 is a circular RNA generated by back-splicing of gene MYO10 and upregulated in osteosarcoma cell lines, but its functional role still unknown. This study aimed to clarify the mechanism circMYO10 osteosarcoma.CircMYO10 expression 10 paired chondroma tissues was assessed quantitative reverse transcription polymerase chain reaction (PCR). The function circMYO10/miR-370-3p/RUVBL1 axis regarding two key characteristics: proliferation endothelial-mesenchymal transition (EMT)....
Circular RNAs (circRNAs) have emerged as significant biological regulators. Herein, we aimed to elucidate the role of an unidentified circRNA (circPDE4B) that is reportedly downregulated in osteoarthritis (OA) tissues.The effects circPDE4B were explored human and mouse chondrocytes vitro. Specifically, RNA pull-down (RPD)-mass spectrometry analysis (MS), immunoprecipitation, glutathione-S-transferase (GST) pull-down, immunoprecipitation RPD assays performed verify interactions between RIC8...
Rationale: Osteoarthritis (OA) is the most common joint disease worldwide. Previous studies have identified imbalance between extracellular matrix (ECM) catabolism and anabolism in cartilage tissue as main cause. To date, there no cure for OA despite a few symptomatic treatments. This study aimed to investigate role of CircCDK14, novel circRNA factor, progression OA, elucidate its underlying molecular mechanisms. Methods: The function CircCDK14 well interaction downstream target...
Abstract Objectives Circular RNAs (circRNAs) are noncoding that compete against other endogenous RNA species, such as microRNAs, and have been implicated in many diseases. In this study, we investigated the role of a new circRNA (circSLC7A2) osteoarthritis (OA). Materials Methods The relative expression circSLC7A2 was significantly lower OA tissues than it matched controls, shown by real‐time quantitative polymerase chain reaction (RT‐qPCR). Western blotting, RT‐qPCR immunofluorescence...
Abstract Background Circular RNAs (CircRNAs) are important and have different roles in disease progression. Herein, we aim to elucidate the of a novel CircRNA (CircZSWIM6) which is upregulated ageing chondrocytes. Methods We verified CircZSWIM6 senescent osteoarthritis (OA) development vitro through knockdown overexpression. RNA pulldown assay binding protein immunoprecipitation were performed identify interaction between Ribosomal S14 (RPS14). The ageing‐related OA also confirmed...
Circular RNAs (circRNAs) have risen to prominence as important regulators of biological processes. This study investigated whether circGNB1 functions a competitive endogenous RNA regulate the pathological process oxidative stress in age-related osteoarthritis (OA).The relationship between expression and stress/OA severity was determined cartilages from OA patients at different ages. The roles progression, its downstream targets were using gain- loss-of-function experiments various...
Osteosarcoma is the most common primary malignant bone tumor in adolescents. While chemotherapy combined with surgery can improve prognosis of some patients, chemo-resistance still a huge obstacle osteosarcoma treatment. Accumulating evidence demonstrates that circular RNAs (circRNAs) are involved cancer progression and metastasis, but their specific role remains mostly undescribed. In this study, we performed circRNA deep sequencing identified 88 distinct circRNAs from human cell lines...
Cartilage homeostasis is essential for chondrocytes to maintain proper phenotype and metabolism. Because adult articular cartilage avascular, must survive in low oxygen conditions, changing tension can significantly affect metabolism proteoglycan synthesis these cells. However, whether long noncoding RNA participate under hypoxia has not been reported yet. Here, we first identified LncZFHX2 as a lncRNA upregulated physiological cartilage, specifically by HIF-1α. knockdown simultaneously...
Abstract Transcriptional co-activator with PDZ-binding motif (TAZ) is a downstream effector of the Hippo signaling pathway that participates in tumorigenesis. The aim this study was to identify miRNA counterpart for TAZ and elucidate mechanism underlying tumorigenic effect TAZ. We demonstrated upregulated osteosarcoma (OS) tissues cell lines, overexpression can induce migration, invasion proliferation. Moreover, miRNA-224 (miR-224), phenocopy functions TAZ, found be overexpression. Further,...
Osteoarthritis (OA) is a degenerative joint disease. Currently, apart from symptomatic treatment or replacement, no other effective treatments for OA exist. The mechanisms underlying remain elusive and require further research. Circular RNAs (circRNAs) are known to be involved in many diseases; however, their function not yet fully understood. Here, we identified novel circRNA, Circ0083429. role of Circ0083429 was confirmed via western blot (WB), quantitative real-time PCR (qRT-PCR),...
Abstract Doxycycline (DOX) has been used extensively in the control of animal diseases, which leads to its large‐scale accumulation water and causes serious pollution problems. In this study, a BODIPY‐based fluorescent probe named DOX‐BOD was synthesized by one‐pot method. The qualitative quantitative detection DOX achieved specific response effect on DOX. showed excellent performance fluorescence intensity decreased with increase concentration. Besides, limit as low 0.91 μM. method based...