Epithelial‑mesenchymal transition (EMT) is a key step in cancer metastasis. B7‑H3, co‑signaling molecule associated with poor prognosis of non‑small cell lung (NSCLC), promotes the metastasis NSCLC by activating EMT process. However, its underlying mechanism remains poorly understood. In present study, it was shown that CRISPR/Cas9‑mediated B7‑H3 deletion downregulated expression class III histone deacetylase, sirtuin‑1 (SIRT1), A549 cells. Accordingly, SIRT1 silencing resulted markedly...

Anti-miR21 and resveratrol (RSV)-loaded mesoporous silica nanoparticles (MSNs) conjugated with hyaluronic acid (HA) were developed to enhance therapeutic efficacy in gastric carcinoma. The surface conjugation of HA, which acts as a targeting ligand the overexpressed CD44 receptor on cancer cells, was clearly identified by presence greyish shell dark MSNs. Confocal laser-scanning microscopy flow cytometry analysis showed higher cellular internalisation HA/RSVmirNP compared RSVmirNP. In vitro...

MicroRNAs (MiRs) are thought to display regulator action in tumor suppression and oncogenesis. miR-144 plays an important role the development of various cancers, such as colorectal cancer, breast lung by targetting different molecules potentially involved many signaling pathways. SMAD4 is a common during progression, it can inhibit cell proliferation promote motility most epithelial cells. The present study focused on effect colon cancer order find novel gene therapy target for treatment...

Gastric cancer (GC) is an aggressive tumor featured by uncontrolled cell proliferation and metastasis. In recent years, long noncoding RNAs (lncRNAs) act as crucial regulators biological markers in multiple cancers. LncRNA TMPO-AS1 has been revealed to be oncogene some Nevertheless, there little known about the role of GC.Reverse transcription-quantitative polymerase chain reaction analysis was used examine expression level GC tissues cells. Cell Counting Kit-8, colony formation, wound...

Soluble sugar and organic acids are key determinants of fruit organoleptic quality directly affect the commodity value economic returns crops. We performed whole-genome sequencing apple varieties Gala Xiahongrou, along with their F1 hybrids, to construct a high-density bin map. Our quantitative genetic analysis pinpointed 53 trait loci (QTLs) related 11 acid traits. identified candidate gene, MdNADP-ME, responsible for malate degradation, in stable QTL on linkage group 15. Sequence revealed...

Colorectal cancer (CRC), characterized by high mortality and incidence rate, is one of the most common types rectum tumors in gastrointestinal tract worldwide. An increasing number investigations indicated that long noncoding RNAs (lncRNAs) have been implicated growth a wide range cancers. Although it has obtained general acceptance lncRNA LINC00511 plays significant role numerous cancers, regulatory mechanism CRC still needs to be explored.Bioinformatics analysis experiments sphere...

Growing evidence suggests that microRNA plays an essential role in the development and metastasis of many tumors, including gastric cancer. Aberrant miR-224 expression has been indicated tumor growth, mechanism promoting proliferation metastatic ability for cancer remains unclear. Accumulating reports mTOR signal pathway important cellular process, such as apoptosis, cell growth proliferation. The goal present study was to identify whether could inhibit migration, invasion, through targeting...

The Shenque acupoint is located in the umbilicus of human body. In body meridians, can regulate functions. was one important acupuncture acupoints for treatment insomnia. However, effect linalool applied at to improve sleep unknown. This study explored hypnotic and sedative effects main component lavender, linalool, on mice rats. latency duration were studied with supra-threshold dose pentobarbital sodium, rate sub-threshold sodium. order further feasibility superiority administered...

Panax ginseng Meyer has been used as a nourishing edible herb in East Asia for thousands of years. 25-OH-PPT was first discovered natural rare triterpenoid saponin stems and leaves by our group. Research found that it showed strong inhibitory effects on α-glucosidase protein tyrosine phosphatase 1B, protected cardiocytes (H9c2) through PI3K/Akt pathway.In the research, order to optimize enrichment process, optimal macroporous resins purification conditions were studied. Meanwhile,...

Ginsenosides have been widely used clinically for many years and were regarded as very safe. However, a few researches on the toxicities of these kinds agents showed that some ginsenosides may side-effect rats or dogs. So it is extremely necessary to further clarify potential toxicity ginsenosides. This study was carried out investigate long-term genotoxicity 25-methoxydammarane-3, 12, 20-triol (25-OCH3-PPD), new derivative ginsenoside, in beagle dogs.Twenty-four dogs divided randomly into...

Colorectal cancer has become a disease with one of the highest incidence rates worldwide, and liver metastasis threatens prognosis colorectal cancer. MicroRNA (miR)‑200 miR‑141 have been confirmed to be aberrantly expressed in cancer, however, whether miR‑200 are involved progression their role remain fully elucidated. The aim present study was investigate patients. A total 380 patients were enrolled, which 142 diagnosed grade I/II 238 III/IV disease. Reverse transcription‑quantitative...

With the changing lifestyle, venous thrombosis (VT) is becoming increasingly prevalent and poses a burden on health economy. Endothelial progenitor cells (EPCs) are recruited into resolving VT. We aimed to investigate effect of plasminogen activator inhibitor 1 (PAI-1) silencing recanalization VT in rat EPCs. EPCs models were cultured treated with negative control-siRNA vector PAI-1-siRNA vector, respectively. 4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, wound-healing...

Abstract Panaxadiol is a dammarane‐type ginsenoside having high ginseng content. The 3‐hydroxy group of panaxadiol (PD) was modified by fatty acids and diacids. panax glycol had enhanced anticancer activity. Twelve PD derivatives were evaluated purified chemical synthesis, column chromatography, co‐synthesis, identification. human leukemia cells THP‐1, HL‐60, prostate cancer cell lines PC‐3 evaluated; tested in vitro MTT assay. results showed that the antitumor activities some on three tumor...

In this paper, the anti-cancer activity and molecular mechanisms of isomers AD-1 AD-2 (20(R)-AD-1, 20(R)-AD-2, 20(S)-AD-1 20(S)-AD-2) were investigated. The results indicated that all four compounds obviously suppressed viability various cancer cells, 20(R)-AD-1 20(R)-AD-2 was significantly better than 20(S)-AD-2, especially for gastric cells (BGC-803). Then, differences in data showed induced apoptosis decreased MMP, up-regulated expression cytochrome C cytosol, transferred Bax to...