A5077780826- Estrogen and related hormone effects
- DNA and Nucleic Acid Chemistry
- Epigenetics and DNA Methylation
- Advanced biosensing and bioanalysis techniques
- Cytokine Signaling Pathways and Interactions
- DNA Repair Mechanisms
- Bioactive Compounds and Antitumor Agents
- Sarcoma Diagnosis and Treatment
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- RNA and protein synthesis mechanisms
- Cancer therapeutics and mechanisms
- Chemical Reactions and Isotopes
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Histone Deacetylase Inhibitors Research
- Molecular Biology Techniques and Applications
- CAR-T cell therapy research
- Protein Degradation and Inhibitors
- Pharmacogenetics and Drug Metabolism
- Carcinogens and Genotoxicity Assessment
- Steroid Chemistry and Biochemistry
- RNA Research and Splicing
- Cancer-related Molecular Pathways
- Air Quality and Health Impacts
Ministry of Health of the Russian Federation
2017-2025
Peoples' Friendship University of Russia
2023-2025
Russian Cancer Research Center NN Blokhin
2015-2024
Centre international de recherche sur le cancer
2023-2024
Ryazan State Medical University named after Academician I.P. Pavlov
2023
Royal College of Surgeons in Ireland
2023
Heliodor Swiecicki Clinical Hospital
2023
Poznan University of Medical Sciences
2023
University College Cork
2023
Pomeranian Medical University
2023
The Monographs produced by the International Agency for Research on Cancer (IARC) apply rigorous procedures scientific review and evaluation of carcinogenic hazards independent experts. Preamble to IARC Monographs, which outlines these procedures, was updated in 2019, following recommendations a 2018 expert advisory group. This article presents key features Preamble, major milestone that will enable take advantage recent procedural advances made during 12 years since last amendments....
Transitions of B-DNA to alternative DNA structures (ADS) can be triggered by negative torsional strain, which occurs during replication and transcription, may lead genomic instability. However, how ADS are recognized in cells is unclear. We found that the binding candidate anticancer drug, curaxin, cellular results uncoiling nucleosomal DNA, accumulation supercoiling conversion multiple regions into left-handed Z-form. Histone chaperone FACT binds rapidly same via SSRP1 subunit...
Abstract Cutaneous atrophy is the major adverse effect of topical glucocorticoids; however, its molecular mechanisms are poorly understood. Here, we identify stress‐inducible mTOR inhibitor REDD1 (regulated in development and DNA damage response 1) as a target glucocorticoids, which mediates cutaneous atrophy. In knockout (KO) mice, all skin compartments (epidermis, dermis, subcutaneous fat), epidermal stem, progenitor cells were protected from atrophic effects glucocorticoids. Moreover,...
Cellular responses to the loss of genomic stability are well-established, while how mammalian cells respond chromatin destabilization is largely unknown. We previously found that DNA demethylation on p53-deficient background leads transcription repetitive heterochromatin elements, followed by an interferon response, a phenomenon we named TRAIN (Transcription Repeats Activates INterferon). Here, report curaxin, anticancer small molecule, destabilizing nucleosomes via disruption histone/DNA...
The synthesis and in vitro cytotoxicity of a series Pt IV complexes with lonidamine as ligand coordinated axial position are described. Lonidamine was found to affect strongly the cytotoxic activity these new complexes, lowering IC 50 values down nanomolar range. Lipophilicity assessed terms log P showed no direct correlation cytotoxicity.
Previously we discovered that among 15 DNA-binding plant secondary metabolites (PSMs) possessing anticancer activity, 11 compounds cause depletion of the chromatin-bound linker histones H1.2 and/or H1.4. Chromatin remodeling or multiH1 knocking-down is known to promote upregulation repetitive elements, ultimately triggering an interferon (IFN) response. Herein, using HeLa cells and applying fluorescent reporter assay with flow cytometry, immunofluorescence staining quantitative RT-PCR,...
Background. Glucocorticoids are widely used in the treatment of acute lymphoblastic leukemia. However, antileukemic effects often accompanied by development serious metabolic and atrophic complications. A safer alternative may be drugs class selective glucocorticoid receptor agonists with a more favorable safety profile. Aim. To evaluate new synephrine derivative vitro on bone marrow blasts patients leukemia compare results clinical response to therapy. Materials methods. Bone were isolated...
Glucocorticoids are widely used for the treatment of hematological malignancies; however, their chronic use results in numerous metabolic side effects. Thus, development selective glucocorticoid receptor (GR) activators (SEGRA) with improved therapeutic index is important. GR regulates gene expression via (1) transactivation that requires homodimer binding to promoters and linked effects (2) transrepression-mediated negative interaction other transcription factors. Novel modulator Compound A...
DNA sequences that can form unusual secondary structures are implicated in regulating gene expression and causing genomic instability. H-palindromes an important class of such intramolecular triplex structure, H-DNA. Within H-palindrome, the H-DNA canonical B-DNA a dynamic equilibrium shifts toward with increased negative supercoiling. The interplay between H- fact process transcription affects supercoiling makes it difficult to elucidate effects upon transcription. We constructed stable...
Glucocorticoids have excellent therapeutic properties; however, they cause significant adverse atrophogenic effects. The mTORC1 inhibitor REDD1 has been recently identified as a key mediator of glucocorticoid-induced atrophy. We performed computational screening connectivity map database to identify putative inhibitors. top selected candidates included rapamycin, which was unexpected because it inhibits pro-proliferative mTOR signaling. Indeed, rapamycin inhibited induction by...
// Ekaterina A. Lesovaya 1,* , Kirill I. Kirsanov Elena E. Antoshina 1 Lubov S. Trukhanova Tatiana G. Gorkova V. Shipaeva 2 Ramiz M. Salimov Gennady Belitsky Mikhail Blagosklonny 3 Marianna Yakubovskaya 1,** and Olga B. Chernova 4,** Department of Chemical Carcinogenesis, Blokhin Cancer Research Center, Moscow, Russia Tartis-Aging LLC, Cell Stress Biology, Roswell Park Institute, Buffalo, NY, USA 4 Everon Biosciences, Inc., * The first two authors made equal contributions to the study **...
Guanine-rich DNA sequences tending to adopt noncanonical G-quadruplex (G4) structures are over-represented in promoter regions of oncogenes. Ligands recognizing G4 were shown stabilize these and drive their formation regulating expression corresponding genes. We studied the interaction several plant secondary metabolites (PSMs) with G4s effects on gene a cellular context. The binding PSMs formed by well-studied oncogene promoters telomeric repeats was evaluated using fluorescent indicator...
Introduction . Ovarian cancer is one of the most common gynecological cancers worldwide, which difficult to diagnose and treat. high mortality rate from ovarian makes development new therapeutic drugs relevant. Ribavirin (RBV), commonly used antiviral agent, revealed anticancer potential, however, it led severe side effects as well. RBV derivatives were previously synthesized tested putative in models hematological malignancies. The aim this study was estimate (MGs) cells vitro Material...
11550 Background: The development of resistance is the one reason for ineffective treatment soft tissue sarcomas (STSs). search new molecular-biological targets, both personalized selection novel targeted drugs and predicting chemoresistance STSs to individual antitumor agents their combinations, a pressing task. Methods: In this study, an experimental in vitro analysis, whole-genome sequencing, single-cell sequencing tumors patients (N = 9) with undifferentiated pleomorphic were performed....
// Kirill I. Kirsanov 1,* , Elena Kotova 2,* Petr Makhov 2 Konstantin Golovine Ekaterina A. Lesovaya 1 Vladimir M. Kolenko Marianna G. Yakubovskaya Alexei V. Tulin Blokhin Cancer Research Center RAMS, Moscow, Russia Fox Chase Center, Philadelphia, PA * These authors contributed equally to this work Correspondence: Tulin, email: Keywords : PARP-1, PARP-1 inhibitors, DNA-binding small molecules, poly(ADP-ribose) Received December 25, 2013 Accepted January 3, 2014 Published Abstract is a...