A5041161409- Heat shock proteins research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Drug-Induced Hepatotoxicity and Protection
- Genomics and Chromatin Dynamics
- Endoplasmic Reticulum Stress and Disease
- Liver Disease Diagnosis and Treatment
- Phytochemistry and biological activity of medicinal plants
- Phytochemistry and Bioactivity Studies
- RNA modifications and cancer
- DNA Repair Mechanisms
- Natural product bioactivities and synthesis
- DNA and Nucleic Acid Chemistry
- Genetic Neurodegenerative Diseases
- Phytochemistry and biological activities of Ficus species
- Pharmacological Effects of Natural Compounds
- Genomics, phytochemicals, and oxidative stress
- Bacterial Genetics and Biotechnology
- Phytochemistry and Biological Activities
- Natural Antidiabetic Agents Studies
- Cancer-related Molecular Pathways
- Ethnobotanical and Medicinal Plants Studies
- NF-κB Signaling Pathways
- Muscle Physiology and Disorders
- Nanoparticle-Based Drug Delivery
Bose Institute
2015-2024
Roswell Park Comprehensive Cancer Center
2001-2023
Cleveland Clinic Lerner College of Medicine
2001-2011
Cleveland Clinic
2001-2005
Jadavpur University
1999-2003
University of Oregon
2003
Cancer Genetics (United States)
2001
University of Lucknow
1997
Large parts of mammalian genomes are transcriptionally inactive and enriched with various classes interspersed tandem repeats. Here we show that the tumor suppressor protein p53 cooperates DNA methylation to maintain silencing a large portion mouse genome. Massive transcription major short, nuclear elements (SINEs) B1 B2, both strands near-centromeric satellite DNAs consisting repeats, multiple species noncoding RNAs was observed in p53-deficient but not wild-type fibroblasts treated...
The quinacrine-related compounds curaxins target multiple procancer pathways through FACT complex.
Transitions of B-DNA to alternative DNA structures (ADS) can be triggered by negative torsional strain, which occurs during replication and transcription, may lead genomic instability. However, how ADS are recognized in cells is unclear. We found that the binding candidate anticancer drug, curaxin, cellular results uncoiling nucleosomal DNA, accumulation supercoiling conversion multiple regions into left-handed Z-form. Histone chaperone FACT binds rapidly same via SSRP1 subunit...
Human Upf1 protein (p), a group 1 RNA helicase, has recently been shown to function in nonsense-mediated mRNA decay (NMD) mammalian cells. Here, we demonstrate that the estimated 3 × 106 copies of hUpf1p per exponentially growing HeLa cell are essentially equally distributed among polysomal, subpolysomal, and ribosome-free fractions. We also binds is phosphoprotein harboring phosphoserine phosphothreonine. phosphorylated highest extent when polysome-associated lowest ribosome free. find...
Light-responsive nanoliposomes are being reported to induce cancer cell death through heat and reactive oxygen species (ROS).
Different concentrations (50, 100, 150 microg/mL) of the methanol extract roots Asparagus racemosus Willd. showed considerable in vitro antibacterial efficacy against Escherichia coli, Shigella dysenteriae, sonnei, flexneri, Vibrio cholerae, Salmonella typhi, typhimurium, Pseudomonas putida, Bacillus subtilis and Staphylococcus aureus. The effects produced by were compared with chloramphenicol.
Transcription factors TFIIB and TFIIF are both required for RNA polymerase II preinitiation complex (PIC) assembly, but their roles at downstream of initiation not clear. We now show that phosphorylated by casein kinase 2 remains competent to support PIC assembly is stably retained in the PIC. PICs completely lacking defective or subsequent promoter clearance, demonstrating clearance. Lack reduces transcription levels some promoters, coincident with reduced retention TFIIB. normally...
Aggregation of proteins with the expansion polyglutamine tracts in brain underlies progressive genetic neurodegenerative diseases (NDs) like Huntington's disease and spinocerebellar ataxias (SCA). An insensitive cellular proteotoxic stress response to non-native protein oligomers is common such conditions. Indeed, upregulation heat shock factor 1 (HSF1) function its target chaperone expression has shown promising results animal models NDs. Using an HSF1 sensitive cell based reporter...
Parkinsons disease (PD) affects millions worldwide, with no efficient therapy currently available. A major cause of the initiation and progression this degenerative is dysfunctional cellular protein quality control system (PQC), leading to accumulation toxic aggregates in neurons. We previously reported azadiradione (AZD), a small molecule (MW 451 Da), as potent inducer heat shock factor 1 (HSF1) activity, which could alleviate toxicity induced by misfolded proteins upregulating levels...
Heat shock factor 1 (HSF1) masters cellular proteostasis under stress by upregulating the expression of molecular chaperones that help refold or degrade misfolded proteins. HSF1 activation involves a monomer-to-oligomer transition and binding to its recognition sequence, heat elements (HSEs) on target gene promoters. activity declines with age as well in neurodegenerative disorders (NDs) such Parkinson disease, highlighting need for strategies restore function. Azadiradione (AZD), limonoid...
An extract of the leaves Ficus racemosa Linn. (Moraceae) was evaluated for hepatoprotective activity in rats by inducing chronic liver damage subcutaneous injection 50% v/v carbon tetrachloride liquid paraffin at a dose 3 mL/kg on alternate days period 4 weeks. The biochemical parameters SGOT, SGPT, serum bilirubin and alkaline phosphatase were estimated to assess function. also comparable standard tonic (Neutrosec).
Aim: Paclitaxel (PTX) has no clinically available oral formulations. Cetyl alcohol is metabolized by dehydrogenase and aldehyde that are overexpressed in cancer cells. So, PTX-encapsulated core–shell nanoparticle of cetyl (PaxSLN) could target the cells through route. Materials & methods: PaxSLN was synthesized using microemulsion template. Efficiency evaluated ALDEFLUOR™, multicellular tumor spheroid formation inhibition assays CT26 colorectal carcinoma animal model. Pharmacokinetics...