A5039920014- Pesticide Residue Analysis and Safety
- Insect and Pesticide Research
- Cancer Immunotherapy and Biomarkers
- SARS-CoV-2 and COVID-19 Research
- Pesticide and Herbicide Environmental Studies
- Immunotherapy and Immune Responses
- Pharmacological Effects of Natural Compounds
- Monoclonal and Polyclonal Antibodies Research
- Plant and animal studies
- COVID-19 Clinical Research Studies
- Immune cells in cancer
- Pharmaceutical and Antibiotic Environmental Impacts
- Viral Infections and Vectors
- Animal Ecology and Behavior Studies
- Immune Cell Function and Interaction
- Mosquito-borne diseases and control
- Gastric Cancer Management and Outcomes
- Clostridium difficile and Clostridium perfringens research
- Tryptophan and brain disorders
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Insect-Plant Interactions and Control
- SARS-CoV-2 detection and testing
- Biochemical effects in animals
- Fungal Plant Pathogen Control
Chinese Academy of Sciences
2016-2025
Czech Academy of Sciences, Institute of Microbiology
2025
Soochow University
2025
Fourth People's Hospital of Changzhou
2025
Peking University
2023-2025
Peking University Cancer Hospital
2023-2025
Peking University People's Hospital
2025
Guangdong Pharmaceutical University
2023-2024
Institute of Microbiology
2016-2024
Shanxi Academy of Building Research
2018-2024
Abstract Cancer immunotherapy by targeting of immune checkpoint molecules has been a research ‘hot-spot’ in recent years. Nivolumab, human monoclonal antibody PD-1, widely used clinically since 2014. However, the binding mechanism nivolumab to PD-1 not yet shown, despite report describing complex structure pembrolizumab/PD-1. It previously speculated that glycosylation is involved recognition. Here we with and evaluate effects N-glycosylation on interactions nivolumab. Structural functional...
Zika virus–specific antibodies isolated from a single infected patient show postexposure protection in mice and reveal targets for therapy.
Significance This study indicates that a subpopulation of tumor cells expresses both PD-1 and PD-L1, which decreases the growth by suppressing canonical signaling pathways, i.e. AKT ERK1/2 pathways. In absence adaptive immune system, cell-intrinsic PD-1/PD-L1 mediates resistance to treatment with FDA-approved anti-PD-1/PD-L1 antibodies activating ERK1/2. These findings provide an additional explanation for cancer immunotherapy.
Abstract Aberrant N6-methyladenosine (m6A) modification has emerged as a driver of tumor initiation and progression, yet how long noncoding RNAs (lncRNA) are involved in the regulation m6A remains unknown. Here we utilize data from 12 cancer types The Cancer Genome Atlas to comprehensively map lncRNAs that potentially deregulated by DNA methylation. A novel methylation–deregulated RNA reader–cooperating lncRNA (DMDRMR) facilitated growth metastasis clear cell renal carcinoma (ccRCC)....
Cholesterol increases the risk of aggressive prostate cancer and has emerged as a potential therapeutic target for cancer. The functional roles cholesterol in metastasis are not fully understood. Here, we found that induces epithelial-to-mesenchymal transition (EMT) through extracellular-regulated protein kinases 1/2 pathway activation, which is mediated by EGFR adipocyte plasma membrane-associated (APMAP) accumulation cholesterol-induced lipid rafts. Mechanistically, APMAP interaction with...
Abstract The rapid expansion of the COVID-19 pandemic has made development a SARS-CoV-2 vaccine global health and economic priority. Taking advantage versatility development, three mRNA candidates have entered clinical trials with two-dose immunization regimen. However, waning antibody response in convalescent patients after infection emergence human re-infection raised widespread concerns about possible short duration protection. Here, we developed nucleoside-modified lipid-encapsulated...
Significance We here established the interaction between PDZ binding motif (PBM) at C terminal of ACE2 and domain sorting nexin 27 (SNX27) solved crystal structure ACE2-PBM/SNX27-PDZ complex. Together with retromer complex, SNX27 was found to regulate homeostasis cell surface under physiological conditions. When endocytic pathway used during SARS-CoV-2 infection, SNX27-retromer sorts ACE2/SARS-CoV-2 complex early endosome prevents it from entering lysosome/late endosome, inhibiting entry...
Monoclonal antibody based immune checkpoint blockade therapies have achieved clinical successes in management of malignant tumors. As the first monoclonal targeting molecules entered into clinics, molecular basis ipilimumab-based anti-CTLA-4 has not yet been fully understood. In present study, we report complex structure ipilimumab and CTLA-4. The showed similar contributions from VH VL binding to CTLA-4 front β-sheet strands. mechanism is that strands contributing B7-1 or B7-2 were occupied...
Abstract SARS-CoV-2 can infect many domestic animals, including dogs. Herein, we show that dog angiotensin-converting enzyme 2 (dACE2) bind to the spike (S) protein receptor binding domain (RBD), and both pseudotyped authentic dACE2-expressing cells. We solved crystal structure of RBD in complex with dACE2 found total number contact residues, atoms, hydrogen bonds salt bridges at interface this are slightly fewer than those human ACE2 (hACE2). This result is consistent fact affinity lower...
Lysosome plays important roles in cellular homeostasis, and its dysregulation contributes to tumor growth survival. However, the understanding of regulation underlying mechanism lysosome cancer survival is incomplete. Here, we reveal a role for histone acetylation-regulated long noncoding RNA termed cell death regulator (LCDR) lung survival, which knockdown promotes apoptosis. Mechanistically, LCDR binds heterogenous nuclear ribonucleoprotein K (hnRNP K) regulate stability...
Fluindapyr is a novel chiral succinate dehydrogenase inhibitor used to control fungal diseases. The enantioselective effects of fluindapyr in paddy ecosystems are unknown. We developed new determination method using ultrahigh performance liquid chromatography tandem mass spectrometry. absolute configuration the enantiomers was identified by an electron circular dichroism model. A husk-based biochar material optimize and establish QuEchERs for soil determination. Under anaerobic conditions,...
Immunometabolism in the tumor microenvironment (TME) and its influence on immunotherapy response remain uncertain colorectal cancer (CRC). We perform immunometabolism subtyping (IMS) CRC patients training validation cohorts. Three IMS subtypes of CRC, namely, C1, C2, C3, are identified with distinct immune phenotypes metabolic properties. The C3 subtype exhibits poorest prognosis both cohort in-house cohort. single-cell transcriptome reveals that a S100A9+ macrophage population contributes...
Currently, monoclonal antibodies (MAbs) targeting the SARS-CoV-2 receptor binding domain (RBD) of spike (S) protein are classified into seven classes based on their epitopes. However, most these seriously impaired by Omicron and its subvariants, especially recent BQ.1.1, XBB derivatives. Identification broadly neutralizing MAbs against currently circulating variants is imperative. In this study, we identified a “breathing” cryptic epitope in S protein, named as RBD-8. Two human MAbs, BIOLS56...
The degradation dynamics and dietary risk assessments of thiamethoxam thiacloprid during Lonicera japonica planting, drying, tea brewing processes were systematically investigated using high-performance liquid chromatography. half-lives 1.0-4.1 d in the honeysuckle flowers leaves, with rate constants k ranging from -0.169 to -0.696. safety interval time was 7 d. sun- oven-drying (70 °C) percent digestions 59.4-81.0% for residues, which higher than shade- percentages at lower temperatures...
Exposure to cold weather is associated with infaust cardiovascular responses, including myocardial infarction and arrhythmias. However, the exact mechanisms of these adverse changes in myocardium under stress are unknown. This study was designed investigate cardiac injury induced by mice. The mice were randomly divided into three groups, normal control (no handling), 1-week 2-week stress. We observed physiological morphological tissues, we measured 3′-nitrotyrosine 4-hydroxynonenal,...
Rice is an important food crop, with a long history of cultivation in China, and one the staple foods for Chinese today. However, occurrence rice diseases has had substantial impact on its yield. At present, chemical control main means prevention diseases. As high-efficacy broad-spectrum fungicide, prothioconazole shows ideal effect common diseases, but residuals that remain after use may have human health. In this paper, ultrahigh-performance liquid chromatography–tandem mass spectrometry...
Polyphyllin I (PPI), a bioactive constituent extracted from the rhizomes of Paris polyphylla, is cytotoxic to several cancer types. This study was designed explore whether PPI prevents non-small-cell lung (NSCLC) growth and investigate molecular mechanism. AMP-activated protein kinase (AMPK) has been implicated in activation autophagy distinct tissues. In cultured human NSCLC cell lines, induces by activating AMPK then inhibiting mTOR signaling concentration-dependent manner. Furthermore,...
Programmed cell death 1 (PD-1)/PD-1 ligand-1 (PD-L1)-blocking monoclonal antibodies (mAbs) have taken center stage for tumor immune checkpoint therapy. Identification of the "hotspots" on PD-1 mAbs will help to develop next-generation oral deliverable agents with long-lasting efficacy. Here, we identified two PD-1-targeting mAbs, GY-5 and GY-14, PD-1/PD-L1-blocking Complex structural information revealed that both mainly bind FG loop PD-1, which also contributes multiple interactions PD-L1....
To explore the effect of microRNA-132 heart failure and provide theoretical guidance for clinical treatment (HF).Peripheral blood was collected from HF patients. RT-qPCR used to determine expression. Mouse models were established. Color Doppler ultrasound utilized measure changes cardiac function. HE Masson staining applied observe pathological myocardium. After H9C2 cells transfected with microRNA-132, MTT assay employed detect stability cells. ELISA levels oxidative stress factors. Western...