A5101987668- Extracellular vesicles in disease
- RNA Interference and Gene Delivery
- IL-33, ST2, and ILC Pathways
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Plant Disease Resistance and Genetics
- Asthma and respiratory diseases
- Mesenchymal stem cell research
- Plant Gene Expression Analysis
- Glaucoma and retinal disorders
- Plant Molecular Biology Research
- Cell death mechanisms and regulation
- SARS-CoV-2 and COVID-19 Research
- Cancer, Lipids, and Metabolism
- Molecular Biology Techniques and Applications
- Virus-based gene therapy research
- Forensic and Genetic Research
- Hematopoietic Stem Cell Transplantation
- Yersinia bacterium, plague, ectoparasites research
- Cancer-related Molecular Pathways
- Lung Cancer Treatments and Mutations
- Eosinophilic Esophagitis
- Pluripotent Stem Cells Research
The First Affiliated Hospital, Sun Yat-sen University
2023-2025
Sun Yat-sen University
2020-2025
Hubei Academy of Agricultural Sciences
2019-2024
Ministry of Agriculture and Rural Affairs
2024
Wenzhou Medical University
2020-2024
Shanghai Jiao Tong University
2012-2024
Affiliated Eye Hospital of Wenzhou Medical College
2020-2024
First Affiliated Hospital of Nanchang University
2024
Nanchang University
2024
Northwest Normal University
2024
Platelet/endothelial cell adhesion molecule 1 (PECAM-1; CD31) is crucial to the process of leukocyte transmigration through intercellular junctions vascular endothelial cells. A monoclonal antibody PECAM, or recombinant soluble blocks transendothelial migration monocytes by 70-90%. Pretreating either with transmigration. If endothelium first activated cytokines, anti-PECAM PECAM again block both and neutrophils. Anti-PECAM does not chemotaxis type. Light electron microscopy reveal that...
Mesenchymal stem cells (MSCs) have shown promise for the therapy of cerebral ischemia in animal studies and clinical trials, yet their application still faces many challenges. Utilizing small extracellular vesicles (sEVs) may overcome these In study, we overexpressed brain-derived neurotrophic factor (BDNF) cultured MSCs purified sEVs using anion exchange chromatography. an ischemic stroke mouse model, selectively targeted peri-infarct region after intranasal administration, BDNF loading...
A murine model of peritonitis was used to test the role platelet/endothelial cell adhesion molecule 1 (PECAM-1/CD31) in acute inflammation. monoclonal antibody (mAb) specific for PECAM-1 injected intravenously 4 h before intraperitoneal injection thioglycollate broth blocked leukocyte emigration into peritoneal cavity up 48 h. This block particularly evident neutrophils. Control mAb, including one that bound CD18 without blocking its function, failed when at same or higher concentrations....
Human umbilical cord mesenchymal stem cells (huMSCs) can treat primary ovarian insufficiency (POI) related to granulosa cell (OGC) apoptosis caused by cisplatin chemotherapy. Exosomes are a class of membranous vesicles with diameters 30-200 nm that constitutively released eukaryotic cells. mediate local cell-to-cell communication transferring microRNAs and proteins. In the present study, we demonstrated effects exosomes derived from huMSCs (huMSC-EXOs) on cisplatin-induced OGC model in vitro...
Quercetin is a hydrophobic agent with potential anticancer activity. The aim of the present study was to observe effects quercetin on proliferation breast cancer cell line MCF-7 and gene expression survivin. molecular mechanism underlying antiproliferative effect also investigated. cells were treated various concentrations quercetin. inhibitory detected using 3-(4,5-dimethylthiazol-2-yl)‑2,5-diphenyltetrazolium bromide (MTT) method inhibition rate calculated. Cellular apoptosis by...
Abstract COVID-19 is identified as a zoonotic disease caused by SARS-CoV-2, which also can cross-transmit to many animals but not mice. Genetic modifications of SARS-CoV-2 or mice enable the susceptible viral infection. Although neither natural situation, they are currently utilized establish mouse infection models. Here we report direct contact transmission variant B.1.351 in wild-type The (B.1.351) replicated efficiently and induced significant pathological changes lungs tracheas,...
Abstract Background Recovery from a foot ulcer is compromised in diabetic status, due to the impaired tissue microenvironment that consists of altered inflammation, angiogenesis and fibrosis. Phenotypic alterations both macrophages fibroblasts have been detected wound. Recently, fibroblast subpopulation expresses high matrix metalloproteinase 1 (MMP1), MMP3, MMP11 Chitinase-3-Like Protein (CHI3L1) was associated with successful wound healing. However, it not known whether these...
Dendritic cells (DCs) and tumor cell-derived exosomes have been used to develop antitumor vaccines. However, the biological properties antileukemic effects of leukemia (LEXs) are not well described. In this study, induction immunity LEXs were investigated using transmission electron microscopy, western blot analysis, cytotoxicity assays, animal studies. Similar other cells, release exosomes. Exosomes derived from K562 (LEXK562) membrane-bound vesicles with diameters approximately 50–100 μm...
Quercetin is a hydrophobic agent that demonstrates potential anticancer activity. The aim of the present study was to observe effects quercetin on proliferation and apoptosis ovarian cancer cell line SKOV-3, provide foundation for treatment using this agent. Ovarian SKOV-3 cells were treated with at different doses. inhibitory effect detected MTT assay inhibition rate calculated. Cell determined Hoechst staining, western blot analysis used analyze changes in expression levels survivin...
Tibeto-Burman (TB) people have endeavored to adapt the hypoxic, cold, and high-UV high-altitude environments in Tibetan Plateau complex disease exposures lowland rainforests since late Paleolithic period. However, full landscape of genetic history biological adaptation geographically diverse TB-speaking people, as well their interaction mechanism remain unknown. We generate a whole-genome meta-database 500 individuals from 39 populations East Asia Southeast present comprehensive diversity,...
The SARS-CoV-2 Delta (B.1.617.2) strain is a variant of concern (VOC) that has become the dominant worldwide in 2021. Its transmission capacity approximately twice original strain, with shorter incubation period and higher viral load during infection. Importantly, breakthrough infections have continued to emerge first-generation vaccine recipients. There thus an urgent need develop novel variants as major target. Here, receptor binding domain (RBD)-conjugated nanoparticle vaccines targeting...
Polymeric micelles co-delivered hydrophilic doxorubicin and hydrophobic curcumin improved cytotoxicity, apoptosis, cellular uptake<italic>in vitro</italic>and enhanced antitumor anti-metastasis activity<italic>in vivo</italic>on breast carcinoma.
Aim: To investigate the mechanisms of induction apoptosis esophageal cancer cells by cancer-related gene 2 (ECRG2) in combination with cisplatin (DDP). Methods: Hoechest staining was performed to analyze effects single ECRG2 and DDP on EC9706 cells. The expression levels p53 bcl-2 mRNA protein were determined RT-PCR Western blotting, respectively. Results: number apoptotic after treatment for 24 hours more than that ECRG2. blotting showed both down-regulated, while up-regulated treated...
(2021). Comparative Evaluation in Intense Pulsed Light Therapy Combined with or without Meibomian Gland Expression for the Treatment of Dysfunction. Current Eye Research: Vol. 46, No. 8, pp. 1125-1131.
ABSTRACT Besides surgical decompression, neuroprotection and neuroinflammation reduction are critical for acute spinal cord injury (SCI). In this study, we prepared small extracellular vesicles (sEVs) from immortalised mesenchymal stem cells overexpressing brain‐derived neurotrophic factor (BDNF) evaluated whether intranasal administration of BDNF‐sEVs is a therapeutic option SCI. cultured neurons, BDNF loading enhanced neurite outgrowth promoted by sEVs. After administration,...
Low-density neutrophils are heterogeneous immune cells with immunosuppressive (such as polymorphonuclear myeloid-derived suppressor [PMN-MDSC]) or pro-inflammatory low-density granulocytes [LDG]) properties that have been well described in multiple cancers and diseases. However, its role allergic rhinitis (AR) is still unclear.
Exosomes secreted by tumor cells contain specific antigens that may have immunotherapeutic purposes. The aim of this study was to characterize the proteomic content lymphoma cell-derived exosomes (LCEXs).In study, derived from Raji (EXO(Raji)) were purified and proteins EXO(Raji) separated one-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Protein bands identified mass spectrometry. protein components analyzed using shotgun technology, function defined described Gene...