A5029413137- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cancer Mechanisms and Therapy
- Respiratory Support and Mechanisms
- Occupational and environmental lung diseases
- Neonatal Respiratory Health Research
- Systemic Sclerosis and Related Diseases
- Pleural and Pulmonary Diseases
- Pulmonary Hypertension Research and Treatments
- PARP inhibition in cancer therapy
- Mechanisms of cancer metastasis
- Cancer Research and Treatments
- Renal cell carcinoma treatment
- Sulfur Compounds in Biology
- Neuroblastoma Research and Treatments
- Antimicrobial Resistance in Staphylococcus
- Antioxidant Activity and Oxidative Stress
- Glioma Diagnosis and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Phytochemistry Medicinal Plant Applications
- Cancer, Hypoxia, and Metabolism
- Melanoma and MAPK Pathways
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Sepsis Diagnosis and Treatment
- Mechanical Circulatory Support Devices
- Skin Diseases and Diabetes
University of Fribourg
2020-2025
Centre for Health Technology and Services Research
2025
Universidade Nova de Lisboa
2025
Institute of Cancer Research
2007-2023
Universidade Metodista de São Paulo
2023
The University of Texas MD Anderson Cancer Center
2021
Universidade de São Paulo
2010-2020
Genomic (Brazil)
2018
Universidade Federal do Rio de Janeiro
2009-2018
Centro Universitário das Faculdades Metropolitanas Unidas
2013-2018
Abstract Small, gaseous molecules such as nitric oxide, carbon monoxide and hydrogen sulfide are produced signalling in mammalian cells. Here, we show that low concentrations of cyanide generated endogenously various tissues We detect several cellular compartments human cells the blood mice. Cyanide production is stimulated by glycine, occurs at pH lysosomes requires peroxidase activity. When a specific rate, exerts stimulatory effects on mitochondrial bioenergetics, cell metabolism...
The authors hypothesized that low tidal volume (VT) would minimize ventilator-induced lung injury regardless of the degree mechanical power. investigated impact power, obtained by different combinations VT and respiratory rate (RR), on in experimental mild acute distress syndrome (ARDS).Forty Wistar rats received Escherichia coli lipopolysaccharide intratracheally. After 24 h, 32 were randomly assigned to be mechanically ventilated (2 h) with a combination (6 ml/kg 11 ml/kg) RR resulted high...
Despite an increase in the knowledge of mechanisms and mediators involved pulmonary fibrosis, there are no successful therapeutics available. Lipoxins (LX) their 15-epimers, aspirin-triggered LX (ATL), endogenously produced eicosanoids with potent anti-inflammatory proresolution effects. To date, few studies have been performed regarding effect on fibrosis. In present study, using C57BL/6 mice, we report that bleomycin (BLM)-induced lung fibrosis was prevented by concomitant treatment ATL...
Our aim was to investigate the role of oxidative stress in elastase-induced pulmonary emphysema. C57BL/6 mice were subjected pancreatic porcine elastase (PPE) instillation (0.05 or 0.5 U per mouse, i.t.) induce Lungs collected on days 7, 14, and 21 after PPE instillation. The control group sham injected. Also, treated with 1% aminoguanidine (AMG) inducible NO synthase (iNOS) knockout received (i.t.), lungs analyzed after. We performed bronchoalveolar lavage, biochemical analyses stress, lung...
The antioxidant effects of Caryocar brasiliense Camb, commonly known as the pequi fruit, have not been evaluated to determine their protective against oxidative damage in lung carcinogenesis. In present study, we role fruit urethane-induced DNA and stress forty 8-12 week old male BALB/C mice. An vivo comet assay was performed assess tissues changes lipid peroxidation redox cycle antioxidants were monitored for stress. Prior supplementation with oil or its extract (15 µL, 60 days)...
Tidal volume (VT) has been considered the main determinant of ventilator-induced lung injury (VILI). Recently, experimental studies have suggested that mechanical power transferred from ventilator to lungs is promoter VILI. We hypothesized that, as long kept below a safe threshold, high VT should not be injurious. The present study aimed investigate impact different levels and respiratory rates (RR) on function, diffuse alveolar damage (DAD), ultrastructure, expression genes related...
Cystathionine β-synthase (CBS), CSE (cystathionine γ-lyase) and 3-mercaptopyruvate sulfurtransferase (3-MST) have emerged as three significant sources of hydrogen sulfide (H2S) in various forms mammalian cancer. Here, we investigated the functional role CBS’ 3-MST’s catalytic activity murine breast cancer cell line EO771. The CBS/CSE inhibitor aminooxyacetic acid (AOAA) 3-MST 2-[(4-hydroxy-6-methylpyrimidin-2-yl)sulfanyl]-1-(naphthalen-1-yl)ethan-1-one (HMPSNE) were used to assess endogenous...
Abstract Neurodegenerative diseases are a major constraint on the social and economic development of many countries. Evidence has suggested that phytochemicals have an impact brain pathology; however, both their mechanisms action cell targets incompletely known. Here, we investigated effects flavonoid casticin, extracted from Croton betulaster , common plant in state Bahia Brazil, rat cerebral cortex neurons vitro. Treatment neural progenitors with 10 μM casticin increased neuronal...
Telomerase is typically expressed in cellular populations capable of extended replication, such as germ cells, tumor and stem but also induced tissue injury, repair fibrosis. Its catalytic component, telomerase reverse transcriptase (TERT) lung fibroblasts from patients with fibrotic interstitial disease rodents bleomycin-induced pulmonary To evaluate the fibroblast specific role TERT fibrosis, transgenic mice bearing a floxed allele were generated, then crossed an inducible collagen...
Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA tumor tissue its relationship with different histological types other components extracellular matrix, such as angiogenesis. We evaluated association between degree malignancy through expression lung sputum. Tumoral had significantly increased compared to normal tissue. Strong staining intensity associated cancer cells was significant squamous...
The immunogenic collagen V (Col V) and the proinflammatory cytokine interleukin (IL)-17 have been implicated in pathogenesis of multiple autoimmune diseases. Col is also up-regulated during adipogenesis can stimulate adipocyte differentiation vitro. Conditioned medium (CM) generated from adipose-derived mesenchymal stem cells (MSCs) reduces bleomycin (BLM)-induced lung injury rats, suggesting a crucial role situ immunomodulatory factors secreted by MSCs these beneficial effects. In present...
Poly(ADP-ribose) polymerase 1 (PARP1), as a potential target for the experimental therapy of acute lung injury (ALI), was identified over 20 years ago. However, clinical translation this concept not possible due to lack clinically useful PARP inhibitors. With introduction several novel, ultrapotent inhibitors, inhibitor repurposing has re-emerged. Here, we evaluated effect 5 clinical-stage inhibitors in oxidatively stressed cultured human epithelial cells and monocytes vitro demonstrated...
The purpose of the study was to evaluate relationship between personal and organizational demands resources supported by Job Demands Resources (JD-R) model. 252 workers participated, 71% women, mean age 34 years (SD = 12) length service 28 months 16). Data collection online. Validity scales a sociodemographic questionnaire evaluated variables. One question perception general health. Descriptive statistics structural equations were performed for data analysis. results confirm model adequacy...
Variable ventilation has been shown to improve pulmonary function and reduce lung damage in different models of acute respiratory distress syndrome. Nevertheless, variable not tested during pneumonia. Theoretically, periodic increases tidal volume (VT) airway pressures might worsen the impairment alveolar barrier usually seen pneumonia could increase bacterial translocation into bloodstream. We investigated impact on histologic damage, as well markers inflammation, epithelial endothelial...
Malignant pleural effusion has few options of treatment and drugs administrated by different routes can lead to a less permissive microenvironment for the development malignant disease.To analyze therapies administered intrapleurally in disease study EGFR KRAS mutations adenocarcinoma.Mice received LLC cells were treated with anti-VEGF, anti-EGFR, anti-VEGF+anti-EGFR or saline. Animal survival, weight mobility, volume, biochemistry immunology fluid, gene expression, mutation evaluated.All...
Prior studies demonstrate the activation of poly-(ADP-ribose) polymerase 1 (PARP1) in various pathophysiological conditions, including sepsis. We have assessed effect olaparib, a clinically used PARP1 inhibitor, on responses human peripheral blood leukocytes (PBMCs) obtained from healthy volunteers response to challenging with live bacteria, bacterial lipopolysaccharide (LPS), or oxidative stress (hydrogen peroxide, H2O2). The viability PBMCs exposed olaparib earlier generation PARP...
To study the expression of COX-1 and COX-2 in remodeled lung systemic sclerosis (SSc) idiopathic pulmonary fibrosis (IPF) patients, correlating that with patient survival.We examined open biopsy specimens from 24 SSc patients 30 IPF using normal tissue as a control. The histological patterns included fibrotic nonspecific interstitial pneumonia (NSIP) usual (UIP) patients. We used immunohistochemistry histomorphometry to evaluate alveolar septa, vessels, bronchioles. then correlated function...
Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor apoptosis. We evaluated the quantitative relationship between index, synthesis, and epithelial/fibroblast replication mice exposed to butylated hydroxytoluene (BHT) at high oxygen concentration. Two groups were analyzed: 20 received BHT, 10 control corn oil. Telomerase expression,...
Aim: To evaluate different intratracheal flow rates on extracellular matrix content and lung mechanics in an established decellularization protocol. Materials & methods: Healthy mice were used: 15 for five to serve as controls. Fluids instilled at 5, 10 20 ml/min through tracheal cannula right ventricular cavity (0.5 ml/min) all groups. Results: The rate better preserved collagen decellularized lungs. Elastic fiber decreased 5 ml/min, but not compared with Chondroitin, heparan dermatan was...