A5023674596- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Water Treatment and Disinfection
- Pesticide Exposure and Toxicity
- Chemical Analysis and Environmental Impact
- Toxic Organic Pollutants Impact
- Cancer Immunotherapy and Biomarkers
- Comparative Animal Anatomy Studies
- Cancer-related gene regulation
- Chromosomal and Genetic Variations
- Selenium in Biological Systems
- Carcinogens and Genotoxicity Assessment
- Circular RNAs in diseases
- MicroRNA in disease regulation
- CAR-T cell therapy research
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Cancer Mechanisms and Therapy
- Birth, Development, and Health
- Genetic and Kidney Cyst Diseases
- Glutathione Transferases and Polymorphisms
Shanghai Chest Hospital
2024-2025
Shanghai Jiao Tong University
2025
First Hospital of China Medical University
2020-2024
China Medical University
2020-2024
Huazhong University of Science and Technology
2013-2023
Ministry of Ecology and Environment
2019-2022
Beijing Radiation Center
2020-2022
Hebei University
2021-2022
Ministry of Education of the People's Republic of China
2020-2021
Weifang Medical University
2020
Research Article10 March 2016Open Access Source DataTransparent process CRISPR/Cas9-mediated somatic correction of a novel coagulator factor IX gene mutation ameliorates hemophilia in mouse Yuting Guan Shanghai Key Laboratory Regulatory Biology, Institute Biomedical Sciences and School Life Sciences, East China Normal University, Shanghai, Search for more papers by this author Yanlin Ma Corresponding Author Hainan Provincial Human Reproductive Medicine Genetic Research, Medical Center, the...
Coronavirus disease 2019 (COVID-19) has spread rapidly and led to over 5 million deaths date globally. Due the successively emerging mutant strains, therapeutics prevention against causative virus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), are urgently needed. Prevention of SARS-CoV-2 infection in public hospital areas is essential reduce frequency infections. Silver nanoparticles (AgNPs) with virucidal effects have been reported. Therefore, we investigated activity...
Genome engineering is a powerful approach to study how chromosomal architecture impacts phenotypes. However, quantifying the fitness impact of translocations independently from confounding effect base substitutions has so far remained challenging. We report novel application CRISPR/Cas9 technology allowing generate with high efficiency both uniquely targeted and multiple concomitant reciprocal in yeast genome. Targeted are constructed by inducing two double-strand breaks on different...
Abnormal RNA m6A methylation is known to lead the occurrence and progression of multiple cancers including gastric cancer (GC). However, integrative effects all regulators on GC prognosis are unclear. Our research aimed globally analyze values 33 in by univariate multivariate Cox regression analyses. Among regulators, fat mass obesity-associated protein (FTO), an demethylase, was identified as a key prognostic risk factor overall survival (OS) patients. It found that FTO could promote cell...
As a vital feature of the microenvironment, hypoxia, especially long-term is known to promote metastasis and lead poor prognosis in solid tumors. Circular RNAs (circRNAs) participate important processes cell proliferation cancers. However, contribution circRNAs under hypoxia obscure. In this study, we aim explore specific functions circHIPK3 hypoxia-promoting gastric cancer (GC). The hypoxic resistant (HRGC) lines established previously, which were tolerant 2% O2 conditions, used as model....
Succinylation is a newly discovered and multienzyme-regulated post-translational modification (PTM) that associated with the initiation progression of cancer. Currently, no systematic analyses on role succinylation regulators in tumors have been reported. In this study, we performed comprehensive pan-cancer analysis four well-known (CPT1A, KAT2A, SIRT5, SIRT7). We found these played specific critical roles prognosis clear cell renal carcinoma (ccRCC). constructed risk score (RS) based two...
Abstract Background Tumor immune evasion extends beyond T cells, affecting innate elements like natural killer cells (NK) and macrophages within the tumor-immune microenvironment (TIME). Nevertheless, translational strategies to trigger collaboration of NK initiate sufficient anti-tumor cytoxicity remain scarce are urgently needed. Methods In this study, TCGA datasets was used confirm prognosis value expression level NLR family pyrin domain containing 4 (NLRP4) in NSCLC tumor tissues...
Colorectal cancer (CRC) is presenting a global public health problem with high incidence and mortality. Early diagnosis treatment are the most important strategies to improve prognosis of this disease. Besides fecal occult blood test (FOBT) colonoscopy, widely used methods for CRC screening currently, more effective early or prognostic prediction needed. Small nucleolar RNAs (snoRNAs) class noncoding (ncRNAs) playing crucial roles in carcinogenesis considered be promising tumor biomarker. In...
Vector systems allowing simultaneously for rapid drug selection, cell labeling, and reporter assay are highly desirable in biomedical research including stem biology. Here, we present such a vector system pCVpf or pCVpr, plasmids that express pf pr, fusion protein between puromycin acetyltransferase green red fluorescent from CV, the human cytomegalovirus enhancer/promoter. Transfection with pCVpr produced ∼10% efficiency of gene transfer. A 2-day pulse selection resulted ∼13-fold enrichment...
Background Neoadjuvant immunotherapy with anti-programmed death-1 (neo-antiPD1) has revolutionized perioperative methods for improvement of overall survival (OS), while approaches major pathologic response patients’ (MPR) recognition along overcoming non-MPR resistance are still in urgent need. Methods We utilized and integrated publicly-available immune checkpoint inhibitors regimens (ICIs) single-cell (sc) data as the discovery datasets, innovatively developed a cell-communication analysis...
The laboratory rat is a valuable mammalian model organism for basic research and drug discovery. Here we demonstrate an efficient methodology by applying transcription activator-like effector nucleases (TALENs) technology to generate Leptin receptor (Lepr) knockout rats on the Sprague Dawley (SD) genetic background. Through direct injection of in vitro transcribed mRNA TALEN pairs into SD zygotes, somatic mutations were induced two three resulting pups. One founders carrying bi-allelic...
Embryonic stem (ES) cells have immortality for self-renewal and pluripotency.Differentiated human undergo replicative senescence.In human, the telomerase reverse transcriptase (Tert), namely catalytic subunit of telomerase, exhibits differential expression to regulate activity governing cellular or senescence, tert is a routine marker pluripotent ES cells.Here we identified medaka gene determined its in vivo vitro.We found that locus produces five variants called terta terte encoding...
Septic liver injury remains a challenge in sepsis treatment. Nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3 (NLRP3) inflammasome activation has been suggested to be major cause of hepatocyte cell death diseases. However, insufficient research performed explore the underlying mechanisms associated with this. In present study, sophocarpine, pharmaceutical monomer originally isolated from Sophora flavescens, was attenuate septic mouse cecal ligation...
Colon cancer (CC) is considered one of the most common and lethal malignancies occurring both in male female. Its widespread prevalence demonstrates need for novel diagnostic prognostic biomarkers CC. Emerging evidence has shown that small nucleolar RNAs play critical roles tumor development. In this study, we investigated expression profile functions SNORD16 Our data showed SNORD16, rather than its host gene (RPL4), was upregulated CC cell lines. Compared to matched adjacent normal tissues,...