A5040358650- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- RNA Interference and Gene Delivery
- Muscle Physiology and Disorders
- Eicosanoids and Hypertension Pharmacology
- Protein Kinase Regulation and GTPase Signaling
- Rabies epidemiology and control
- Cardiomyopathy and Myosin Studies
- interferon and immune responses
- Nitric Oxide and Endothelin Effects
- Chromosomal and Genetic Variations
- Immune Cell Function and Interaction
- Signaling Pathways in Disease
- Immune Response and Inflammation
- MicroRNA in disease regulation
- Plant Virus Research Studies
St Vincents Institute of Medical Research
2018-2024
The University of Melbourne
2015-2022
Australian Centre for Disease Preparedness
2014-2017
Commonwealth Scientific and Industrial Research Organisation
2014-2017
Bats harbor many emerging and reemerging viruses, several of which are highly pathogenic in other mammals but cause no clinical signs disease bats. To determine the role interferons (IFNs) ability bats to coexist with we sequenced type I IFN locus Australian black flying fox, Pteropus alecto, providing what is, our knowledge, first gene map region any bat species. Our results reveal a contracted family consisting only 10 IFNs, including three functional IFN-α loci. Furthermore, genes...
Abstract The RNase III enzyme Drosha has a central role in microRNA (miRNA) biogenesis, where it is required to release the stem-loop intermediate from primary (pri)-miRNA transcripts. However, can also cleave stem-loops embedded within messenger (m)RNAs. This destabilizes mRNA causing target gene repression and appears occur primarily stem cells. While pri-miRNA have been extensively studied, such non-canonical substrates of yet be characterized detail. In this study, we employed...
Abstract Background Bats are a major reservoir of emerging infectious viruses. Many these viruses highly pathogenic to humans however bats remain asymptomatic. The mechanism by which control viral replication is unknown. Here we utilize an integrated approach proteomics informed transcriptomics compare the response immortalized bat and human cells following infection with bat-borne Hendra virus (HeV). Results host between cell lines was significantly different at both mRNA protein levels....
In recent years, bats have been identified as a natural reservoir for diverse range of viruses. Nelson Bay orthoreovirus (NBV) was first isolated from the heart blood fruit bat (Pteropus poliocephalus) in 1968. While pathogenesis NBV remains unknown, other related members this group caused acute respiratory disease humans. Thus potential to impact human health appears plausible. Here, increase our knowledge NBV, we examined replication and infectivity using different mammalian cell lines...
Intracellular signaling mechanisms affected by endothelin (ET), a hypertrophic agonist, and platelet-derived growth factor (PDGF)-BB, proliferative in vascular smooth muscle cells were examined. PDGF-BB was potent mitogen compared with untreated cultures, stimulating both [3H]thymidine incorporation cell number. In contrast, ET poor mitogen, enhancing but not Simultaneous treatment significantly more effective than either agonist alone at uptake Although or stimulated arachidonic acid...
Bats are recognised as an important reservoir for a number of highly pathogenic zoonotic viruses. While many these viruses cause severe and often fatal disease in humans, bats able to coexist with without clinical signs disease. The mechanism conferring this antiviral response is not fully understood. Here, we investigated the differential protein expression immortalised Pteropus alecto kidney cells (PaKiT03) following transfection viral mimic, Poly I:C. Two complementary proteomic...
Nelson Bay orthoreovirus (NBV) is a fusogenic bat borne virus with an unknown zoonotic potential. Previous studies have shown that NBV can infect and replicate in wide variety of cell types derived from their natural host (bat), as well human, mouse monkey. Within permissive cells, induced significant cytopathic effects characterised by cell-cell fusion syncytia formation. To understand the molecular events underpin infection we examined transcriptome proteome response two types, (PaKiT03)...
Class II myosin complexes are responsible for muscle contraction as well other non-sarcomeric contractile functions in cells. Myosin heavy chain molecules form the core of these structures, while light regulate their stability and function. MYL9 is a isoform that thought to myosin. However, whether this only specific cell types or all cells remains unclear. To address this, we generated deficient mice. These mice die soon after birth with abnormalities multiple organs. All exhibited...
ABSTRACT The RNase III enzyme Drosha has a central role in microRNA (miRNA) biogenesis, where it is required to release the stem-loop intermediate from primary (pri)-miRNA transcripts. However, can also cleave stem-loops embedded within messenger (m)RNAs. This destabilizes mRNA causing target gene repression and appears occur primarily stem cells. While pri-miRNA have been extensively studied, such non-canonical substrates of yet characterized detail. In this study, we employed...