A5042509268- Cardiovascular Function and Risk Factors
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- Cardiovascular Effects of Exercise
- Cardiovascular Disease and Adiposity
- Adipose Tissue and Metabolism
- Pancreatic function and diabetes
- Ion channel regulation and function
- Cardiac Fibrosis and Remodeling
- Connexins and lens biology
- Cardiac Imaging and Diagnostics
- Autophagy in Disease and Therapy
- Elasticity and Material Modeling
- Signaling Pathways in Disease
- Fatty Acid Research and Health
- Hormonal Regulation and Hypertension
- Genetics and Neurodevelopmental Disorders
- Neuroscience and Neuropharmacology Research
- Cardiac Ischemia and Reperfusion
- EEG and Brain-Computer Interfaces
- Menopause: Health Impacts and Treatments
- Blood properties and coagulation
- Lipid metabolism and biosynthesis
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
The University of Melbourne
2015-2024
La Trobe University
2021-2022
University Medical Center Utrecht
2014-2016
Charles University
2012
Medical University of Graz
2012
Leiden University Medical Center
2012
Institute of Cardiology
2012
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2012
University College London
2012
Abstract Diabetic cardiomyopathy is a distinct pathology characterized by early emergence of diastolic dysfunction. Increased cardiovascular risk associated with diabetes more marked for women, but an understanding the role dysfunction in female susceptibility to diabetic lacking. To investigate sex-specific relationship between systemic status and vivo occurrence dysfunction, was induced male mice streptozotocin (5x daily i.p. 55 mg/kg). Echocardiography performed at 7 weeks post-diabetes...
Background Among the growing numbers of patients with heart failure, up to one half have failure preserved ejection fraction ( HF p EF ). The lack effective treatments for is a substantial and escalating unmet clinical need—and ‐specific animal models represents major preclinical barrier in advancing understanding . As established reduced r ) proven ineffective , contention that intrinsic cardiomyocyte phenotype distinct these 2 conditions requires consideration. Our goal was validate...
We sought to identify the impacts of Friedreich's ataxia (FRDA) on cardiomyocytes. FRDA is an autosomal recessive degenerative condition with neuronal and non-neuronal manifestations, latter including progressive cardiomyopathy left ventricle, leading cause death in FRDA. Little known about cellular pathogenesis Induced pluripotent stem cells (iPSCs) were derived from three individuals characterized GAA repeats. The differentiated into cardiomyocytes assess phenotypes. iPSC- retained low...
In mice, the calcium-dependent phosphatase calcineurin A (CnA) induces a transcriptional pathway leading to pathological cardiac hypertrophy. Interestingly, induction of CnA has been frequently noticed in human hypertrophic and failing hearts. Independently, arrhythmia vulnerability such hearts regularly associated with remodeling parameters determining electrical conduction (expression level connexin43 (Cx43) NaV1.5, connective tissue architecture), for which precise molecular basis...
Aims Synchronous beating of the heart is dependent on efficient functioning cardiac intercalated disk (ID). The ID composed a complex protein network enabling electrical continuity and chemical communication between individual cardiomyocytes. Recently, several different studies have shed light increasingly prevalent diseases involving ID. Insufficient knowledge its composition makes it difficult to study these disease mechanisms in more detail therefore here we aim expand proteome. Here,...
Aim In healthy hearts, ventricular gap junctions are mainly composed by connexin43 (Cx43) and localize in the intercalated disc, enabling appropriate electrical coupling. diseased Cx43 is heterogeneously down-regulated, whereas activity of calmodulin/calcium-calmodulin protein kinase II (CaM/CaMKII) signalling increases. It unclear if CaM/CaMKII affects expression/localization or impulse propagation. We analysed different models to assess this. Methods results AC3-I mice with CaMKII...
Diastolic dysfunction is increasingly identified as a key, early onset subclinical condition characterizing cardiopathologies of rising prevalence, including diabetic heart disease and failure with preserved ejection fraction (HFpEF). characterization has important prognostic value in management outcomes. Validated tools for vivo monitoring diastolic function rodent models diabetes are required progress pre-clinical cardiology studies. 2D speckle tracking echocardiography emerged powerful...
This study aimed to prospectively examine cardiac structure and function in the kainic acid-induced post-status epilepticus (post-KA SE) model of chronic acquired temporal lobe epilepsy (TLE), specifically for changes between pre-epileptic, early epileptogenesis stages. We also whether any related seizure frequency individual animals.Four hours SE was induced 9 male Wistar rats at 10 weeks age, with 8 saline treated matched control rats. Echocardiography performed prior induction SE, two-...
Abstract The role of sex steroids in cardioprotection is contentious, with large clinical trials investigating hormone supplementation failing to deliver outcomes expected from observational studies. Mechanistic understanding androgen/estrogen myocardial actions lacking. Using a genetic model aromatase tissue deficiency (ArKO) female mice, the goal this investigation was evaluate capacity shift cardiac endogenous steroid conversion influence ischemia-reperfusion resilience by optimizing...
ABSTRACT Cardiometabolic syndromes including diabetes and obesity are associated with occurrence of heart failure diastolic dysfunction. There no specific treatments for dysfunction, therapies to manage symptoms have limited efficacy. Understanding the cardiomyocyte origins dysfunction is an important priority identify new therapeutics. The investigative goal was experimentally define in vitro stiffness properties isolated cardiomyocytes derived from rodent hearts exhibiting vivo response...
A definitive understanding of the role dietary lipids in determining cardioprotection (or cardiodetriment) has been elusive. Randomized trial findings have variable and sex specificity interventions not determined. In this investigation sex-selective cardiac functional effects three diets enriched by omega-3 or omega-6 polyunsaturated fatty acids (PUFA) to an equivalent extent saturated acid components were examined rats after 8-wk treatment period. females myocardial membrane...
Transmural action potential duration differences and transmural conduction gradients aid the synchronization of left ventricular repolarization, reducing vulnerability to reentry arrhythmias. A high-fat diet associated accumulation pericardial adipose tissue are linked with slowing greater arrhythmia vulnerability. It is predicted that cardiac adiposity may more readily influence epicardial (versus endocardial) disrupt normal activation/repolarization gradients. The aim this investigation...
Summary Diabetic heart disease is highly prevalent and characterized by diastolic dysfunction. The mechanisms of diabetic are poorly understood no targeted therapies available. Here we show that the myocardium (type 1 type 2) marked glycogen elevation ectopic cellular localization - a paradoxical metabolic pathology given suppressed cardiomyocyte glucose uptake in diabetes. We demonstrate involvement glycogen-selective autophagy pathway (‘glycophagy’) defect mediating this pathology....
1. Abstract Protein phosphatase 2A (PP2A) regulatory subunit B55α has been implicated in the transcriptional regulation of cardiac growth and fibrosis by suppressing HDAC5/MEF2 signalling cardiomyocytes. We created characterised two mouse models with global or cardiomyocyte-specific disruption gene encoding ( Ppp2r2a ) to conduct first detailed exploration heart. Global homozygous knockout mice died utero , while heterozygous had thinner left ventricular walls at 12 months, an effect more...
Protein phosphatase 2A (PP2A) regulatory subunit B55α has been implicated in the transcriptional regulation of cardiac growth and fibrosis by suppressing HDAC5/MEF2 signalling cardiomyocytes. We created characterised two mouse models with global or cardiomyocyte-specific disruption gene encoding (Ppp2r2a) to conduct first detailed exploration heart. Global homozygous knockout mice died utero, while heterozygous had thinner left ventricular walls at 12 months, an effect more pronounced males....
Diabetic heart disease is highly prevalent 1 and associated with the early development of impaired diastolic relaxation. The mechanisms diabetic are poorly understood it a condition for which there no targeted therapies. Recently, disrupted glycogen-autophagy (glycophagy) glycogen accumulation have been identified in heart. 2 Glycophagy involves receptor binding linking an ATG8 protein to locate degrade within intracellular phago-lysosome. 3,4 Here we show that STBD1...