Radioresistance remains a major challenge in the treatment of glioblastoma multiforme (GBM). Recent data strongly suggests important role miRNAs cancer progression and therapeutic response. Here, we have established radioresistant human GBM cell line U87R derived from parental U87 found miR-135b expression was upregulated cells. knockdown reversed radioresistance cells, overexpression enhanced Mechanically, bioinformatics analysis combined with experimental demonstrated GSK3β (Glycogen...

Valproic acid (VPA), an established antiepileptic drug, was assessed for antitumor activity, including its effects on glioblastoma, but role has not been determined.In the present study, we investigated VPA-induced apoptosis human U87 cells by cell viability, lactate dehydrogenase (LDH) release, TUNEL/Hoechst staining and flow cytometric in vitro, then further explored underlying molecular mechanisms using selective antagonists PD98059, LY294002 SB216763.The data showed that VPA...

Abstract Temozolomide (TMZ) is the internationally recognized and preferred drug for glioma chemotherapy treatment. However, TMZ resistance in appears after long-term use an urgent problem that needs to be solved. Circular RNAs (circRNAs) are noncoding play important role pathogenesis progression of tumors. Hsa_circ_0110757 was identified TMZ-resistant cells by high-throughput sequencing analysis derived from reverse splicing myeloid cell leukemia-1 (Mcl-1) exons. The hsa_circ_0110757...

Reorganization of cytoskeletal-membrane interactions during contractile stimulation may contribute to the regulation airway smooth muscle contraction. We investigated effect on phosphorylation actin-membrane attachment proteins talin, vinculin, and paxillin. Stimulation 32P-labeled canine tracheal strips with acetylcholine (ACh; 10(-3) M) resulted in a rapid 2.6-fold increase serine and/or threonine residues, compared resting levels 0.22 mol PO4(3-)/mol talin. After ACh, tyrosine residues...

Gliomas are the most common type of primary brain tumors. CircRNA ephrin type-B receptor 4 (circEPHB4) is a circular RNA derived from tyrosine kinase EPHB4. However, clinical significance and specific roles circEPHB4 in gliomas glioma cancer stem cells (CSC) have not been studied. Here, we found that (hsa_circ_0081519) SOX10 were up-regulated microRNA (miR)-637 was down-regulated tissues cell lines. Consistently, positively correlated with but negatively miR-637. The altered expressions...

// Songhua Xiao 1, * , Zhen Yang 2, Xingsheng Qiu 3, Ruiyan Lv 1 Jun Liu Ming Wu 4 Yiwei Liao Qing Department of Neurology, Sun Yat-Sen Memorial Hospital, University, Guanzhou, Guangdong, China 2 Hypertension and Vascular Disease, The First Affiliated Guangzhou, 3 Radiation Oncology, Yat-sen Neurosurgery, Xiangya Central South Changsha, Hunan, These authors have contributed equally to this work Correspondence to: Liu, email: chingliucn@yahoo.com Keywords: glioblastoma, TMZ, MGMT, miR-29c,...

Background: MicroRNAs play important roles in cancer progression including glioma. In this study, we aimed to explore the expression pattern, prognostic potential, and functional role of miR-526b-3p human Materials methods: The glioma tissues adjacent non-tumor was determined by quantitative RT-PCR. chi-square test performed evaluate statistical associations between level patient characteristics. value analyzed Kaplan-Meier Cox regression analyses. function MTT, colony formation assay,...

Mitochondrial serine hydroxyl-methyltransferase 2 (SHMT2), participating in the synthesis of mitochondrial thymidine monophosphate, has been reported to drive glioma cell survival ischemia. However, its clinical relevance gliomas remains unclear. In current study, immunohistochemistry was performed examine subcellular localization and expression levels SHMT2 protein non-neoplastic brain tissue specimens. Then, associations with various clinicopathological features patients' prognosis were...

Objective: Ventricular infection from multidrug-resistant (MDR) Acinetobacter baumannii (A. baumannii) is one of the most severe complications craniotomy. However, availability effective therapeutic options for these infections limited. Thus, this report aims to describe efficacy abscess clearance by intraventricular and intravenous tigecycline therapy in managing patients with A. ventriculitis after neurosurgery. Moreover, current literature on use life-threatening reviewed summarized, a...

Background: Cancer cells are frequently addicted to deregulated oncogenic protein translation that usually arises as a consequence of increased signaling flux from eIF4F activation. The small molecule 4EG-I, potent inhibitor initiation through disrupting eIF4E/eIF4G interaction, has been shown exert anticancer effects in animal models human cancers. Methods: Here, we extensively investigated the activity 4EGI-1 glioma U87 cells. anti-cancer were measured by cell viability, lactate...

Valproic acid (VPA), a drug widely used to treat manic disorder and epilepsy, has recently shown neuroprotective effects in several neurological diseases, particularly Parkinson's disease (PD). The goal of the present study was confirm VPA's dose-dependent propensities MPP+ model PD primary dopamine (DA) neurons investigate underlying molecular mechanisms using specific mitogen-activated protein kinases (MAPKs) phosphatidylinositol 3-kinase- (PI3K-) Akt signaling inhibitors. VPA reversed...

Calorie restriction (CR) promotes longevity among distinct organisms from yeast to mammals. Although CR-prolonged lifespan is believed associate with enhanced respiratory activity, it apparently controversial for accelerated energy consumption regardless of insufficient nutrient intake. In reconciling the contradiction less food supply versus much metabolite dispense, we revealed a CR-based mode dual-phase responses that encompass phase mitochondrial enhancement (ME) and post-mitochondrial...

Accumulating evidence has highlighted the correlation between microRNAs (miRNAs) and progression of glioma. However, role miR cluster MC-let-7a-1 ~ let-7d in glioma remains elusive. Thus, current study aimed to investigate effect on progression.Microarray data analysis provided indicating involvement via STAT3. The expression let-7a-1, let-7d, let-7f-1, was diminished tissues cell lines. Additionally, our results revealed that STAT3 a target gene which further verified by dual-luciferase...

Calorie restriction is known to extend lifespan among organisms by a debating mechanism underlying nitric oxide-driven mitochondrial biogenesis. We report here that oxide generators including artemisinin, sodium nitroprusside, and L-arginine mimics calorie resembles hydrogen peroxide initiate the signaling cascades elicit global antioxidative responses in mice. The large quantities of antioxidant enzymes are correlated with low levels reactive oxygen species, which allow down-regulation...

CEP2 (CDC42EP2) is a member of the CDC42 subfamily that belongs to Rho family.The family plays an important role in variety cellular processes including skeletal myogenesis.Here, we find expression increased significantly during C2C12 myogenesis.Overexpression could attenuate myoblast differentiation, while knockdown by siRNA results enhancing myogenesis.Furthermore, demonstrate for first time attenuates differentiation via suppression muscle regulatory factors (MRFs) rather than influencing...

The purpose of the present report was to examine possibility molecular pathological subtyping mucinous adenocarcinomas (MAC) colorectum. Thirty‐five formalin‐fixed and paraffin‐embedded MAC specimens colorectum were analyzed. Genetic alterations p53 gene microsatellite instability (MSI) as well immunohistochemical analysis mucin subtypes (human gastric (HGM), anti‐mucin monoclonal antibody recognizing gland mucous cells‐1, MUC2, CD10) expression levels human mutL homolog 1 (hMLH1), Ki‐67...

The actin-sequestering proteins, thymosin beta-4 (Tβ4) and hypoxia-inducible factor (HIF)-1α, are known to be associated with angiogenesis after myocardial infarction (MI). Herein, we aimed identify the mechanism of HIF-1α induction by Tβ4 investigate effects bone marrow mesenchymal stromal cells (BMMSCs) transfected gene (TMSB4) in a rat model MI.Rat BMMSCs were isolated, cultured, TMSB4 using lentivirus-mediated method. Rats surgically induced MI randomly divided into three groups (n =...

Endomyocardial fibrosis (EMF) is a neglected cardiovascular disease of poverty which carries poor prognosis with no specific treatment affecting mainly children and young adults. Here, we report our 10-year experience in the therapeutic management surgical for EMF.From February 2009 to 2019 March, 55 patients diagnosed EMF from cardiology unit underwent repair at department's pediatric division. There were 35 male, 20 female whose ages varied 1 year 2 months 12 years mean age 5.7 (±3.2). We...

<i>Objective:</i> Mucinous adenocarcinomas (MACs) of the stomach usually show an invasive expansive growth and a poor prognosis. We examined possibility molecular pathological subtyping MACs stomach. <i>Methods:</i> Forty-one formalin-fixed paraffin-embedded MAC specimens were analyzed. Mucin subtypes (MUC2, CD10, HGM, M-GGMC-1) expression levels hMLH1, p53 Ki-67 analyzed by immunohistochemistry as well genetic alterations in <i>p53</i> gene microsatellite...

Calorie restriction (CR) is known to extend lifespan among organisms with the putative mechanism underlying nitric oxide (NO)-enhanced mitochondrial biogenesis. However, whether NO maintains telomere intact that implicated in life expectancy remains unknown. We report here artemisinin derivative artesunate a low concentration up-regulates SIRT3-SOD2 expression global activation of antioxidative networks via signaling cascade AMPK→Akt→eNOS→SIRT1→PGC-1α. While donor sodium nitroprusside and...