A5100389495- Circular RNAs in diseases
- Cancer Mechanisms and Therapy
- RNA Research and Splicing
- Renal and related cancers
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Ion channel regulation and function
- Neuroscience and Neuropharmacology Research
- Inflammasome and immune disorders
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- RNA and protein synthesis mechanisms
- Technology and Security Systems
- Extracellular vesicles in disease
- Cancer-related gene regulation
- Epilepsy research and treatment
- Bioactive natural compounds
- Ubiquitin and proteasome pathways
- Macrophage Migration Inhibitory Factor
- RNA modifications and cancer
- Protein Degradation and Inhibitors
- Receptor Mechanisms and Signaling
- Pregnancy and preeclampsia studies
- TGF-β signaling in diseases
- Retinoids in leukemia and cellular processes
Yunnan University
2024
Union Hospital
2022
Jilin University
2022
Ningbo No.6 Hospital
2019-2021
Xiangya Hospital Central South University
2020-2021
Central South University
2020-2021
Shanghai University
2018-2019
Jining First People's Hospital
2017
Chongqing Vocational Institute of Engineering
2012
Second Affiliated Hospital of Zhejiang University
2011
Gliomas are the most common type of primary brain tumors. CircRNA ephrin type-B receptor 4 (circEPHB4) is a circular RNA derived from tyrosine kinase EPHB4. However, clinical significance and specific roles circEPHB4 in gliomas glioma cancer stem cells (CSC) have not been studied. Here, we found that (hsa_circ_0081519) SOX10 were up-regulated microRNA (miR)-637 was down-regulated tissues cell lines. Consistently, positively correlated with but negatively miR-637. The altered expressions...
AbstractThe ras mutation, which is observed in 20-30% of human non-small cell lung cancers (NSCLCs), one common genetic alterations and has been proposed to be a prognostic factor cancer. Oncogene appears essential for tumor progression maintenance. Several therapeutic agents have developed inhibit ras, such as FTIs antisense oligonucleotides. A new tool blocking oncogenes cancer cells emerged with the discovery that RNA interference can specifically silence expression endogenous genes. In...
Although matrine, a primary active component of dried Sophora flavescens root (ku shen), is known to induce apoptosis in variety tumor cells vitro , the molecular mechanism such remains elusive. This analysis cell cycle and matrine‐treated human gallbladder carcinoma (GBC‐SD) showed that matrine can indeed inhibit proliferation G1 arrest dose‐ time‐dependent manner. An additional western blot also caspase‐3 Bcl‐2 activation, as well cyclinE down‐regulation. Overall, results indicate perturbs...
BackgroundColorectal cancer (CRC) is one of the most common malignancies, and pyroptosis exerts an immunoregulatory role in CRC. Although location primary tumor a prognostic factor for patients with CRC, mechanisms left- right-sided CRC remain unclear.MethodsExpression clinical data were collected from The Cancer Genome Atlas Gene Expression Omnibus databases. Differences characteristics, immune cell infiltration, somatic mutations between then compared. After screening differentially...
Various small molecule GLP1R agonists have been developed and tested for treating type 2 diabetes (T2DM) obesity. However, many of these new compounds drawbacks, such as potential hERG inhibition, lower activity compared to natural GLP-1, limited oral bioavailability in cynomolgus monkeys, short duration action. Recently, a category 3-phenyloxetane derivative with enhanced inhibition has discovered. Using an AIDD/CADD method, compound 14 (DD202-114) was identified potent selective agonist,...
Hematopoietic progenitor kinase 1 (HPK1, MAP4K1), a serine/threonine (SER/THR) kinase, has been identified as negative immune regulator of T-cell receptor signaling. Deprivation the HPK1 function suppresses tumor growth, providing an attractive strategy for cancer immunotherapy. Herein, we present novel PROTAC-based degrader compound DD205-291 with high selectivity and potency. showed dose-dependent inhibition SLP-76 phosphorylation induction IL-2 IFN-γ. Compared other inhibitors, exhibited...
Abstract Aims Idiopathic membranous nephropathy (IMN) is a common cause of adult nephrotic syndrome. Currently, the diagnosis IMN mainly depends on renal biopsy, which invasive. What’s more, markers already known for clinical are not sensitive enough. The present study aims to investigate profiling urinary exosomal circular RNAs (circRNAs) IMN, and look potential biomarker IMN. Methods Urine exosomes were collected from patients with idiopathic syndrome (INS), as well healthy controls (HCs)...
This paper describes a new protein chip method for detection of single-base mismatches and unpaired bases DNA, using genetic fusion molecular system Trx-His6-Linker peptide-Strep-tagII-Linker peptide-MutS (THLSLM). The THLSLM coding sequence was constructed by attaching Strep-tag II mutS gene to pET32a (+) sequentially with insertion linker peptide before behind Strep-tagII gene, respectively. expressed in E. coli AD494 (DE3) purified Ni(2+)-chelation affinity resin. retained both mismatch...
Background: The objective of the present study was to evaluate role TGFβ/PDCD4/AP-1 pathway in nasopharyngeal carcinoma (NPC) and its relationship NPC prognosis. Methods: tissues collected from 66 patients were compared 17 mucosa biopsy specimens as normal tissues. Immunohistochemical staining performed assess expression transforming growth factor-β receptor I (TGFβRI), programmed cell death 4 (PDCD4) activator protein-1 (AP-1). Kaplan-Meier method applied patient overall survival (OS)...
Gastric cancer (GC) has a poor prognosis due to the lack of ideal tumor markers. Circular RNAs (circRNAs) are novel type noncoding RNA related occurrence GC. Among our research, we investigated role hsa_circ_0005556 in The expression 100 paired GC tissues and adjacent normal was detected using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). A receiver operating characteristic (ROC) curve established evaluate diagnostic value hsa_circ_0005556. correlation between...
Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene whose reduced expression may play an important role in the development and progression of esophageal squamous cell cancer (ESCC). The aim this study was to investigate clinical relevance RUNX3 ESCC patients effects overexpression on biological behaviour Eca109 cells vitro vivo. Immunohistochemistry performed detect lymph node metastasis 80 tissues 40 non-cancerous using SP method. RT-PCR Western blotting were applied...
Background. Nephrotic syndrome is an enormous public healthy threaten, which causes a variety of complications and secondary disease; however, the molecular mechanism nephrotic remains unclear. Methods. In our study, RNA-seq were used to test transcription level patients with syndrome, in order investigate interaction circRNA-miRNA-mRNA patients. Results. Consistent hypothesis, miRNAs confirmed be associated majority their targeting circRNAs downregulated at same time, KEGG pathway analysis...
Colorectal cancer (CRC) is one of the leading causes cancer-related death around world, becoming a severe public health problem. Mounting evidence has proven that circRNAs act as pivotal modulators in initiation and development CRC. Although function circTADA2A been explored osteosarcoma breast cancer, specific role CRC remains unknown.Bioinformatics analysis based on GEO datasets was used to evaluate dysregulated CCK-8 transwell assays were detect functions cells. qRT-PCR Western blot...
Objective To investigate the MUC1 expression in endometrial carcinoma and its clinical significance. Methods Twenty specimen of normal proliferative phase, twenty atypical hyperplasia fifty eight were studied. Immunohistochemistry was used to study MUC1. Results The positive rates group 46.6% (27/58)and 35.0% (7/20), significantly higher than that (5% ,4/20). In rate FIGO stage Ⅱ Ⅲ respectively 57.9% (11/19) 87.5% (7/8), Ⅰ (38.7% ,12/31), P ＜0.05 ,the cases deep myometrial invasion (78.3% ,...
Abstract Aims Idiopathic membranous nephropathy (IMN) is a common cause of adult nephrotic syndrome. Currently, the diagnosis IMN mainly depends on renal biopsy, which invasive. What’s more, markers already known for clinical are not sensitive enough. The present study aims to investigate profiling urinary exosomal circular RNAs (circRNAs) IMN, and look potential biomarker IMN. Methods Urine exosomes were collected from patients with idiopathic syndrome (INS), as well healthy controls (HCs)...