Background: For more than 30 years it has been known that gastric acid secretion is inversely related to the extent and severity of corpal gastritis. We therefore evaluated effect cure Helicobacter pylori infection on basal pentagastrin-stimulated secretion. Methods: Basal output (BAO) maximal (MAO) were assessed in 11 H. pylori-infected dyspeptic patients (8 women 3 men; mean age, 28 years) before after successful anti-H. therapy. Results: The gastritis index was significantly lower therapy...

Nonalcoholic fatty liver disease (NAFLD) is the most frequent associated with abnormal tests that characterized by a wide spectrum of damage, ranging from simple macro vesicular steatosis to steatohepatitis (NASH), cirrhosis or carcinoma. Liver biopsy precise test differentiate NASH other stages NAFLD, but it an invasive and expensive method. This study aimed create clinical laboratory score capable identify individual in severely obese patients submitted bariatric surgery.The medical...

This open-label, prospective, phase 2 study evaluated the safety and efficacy of deferasirox (10 ± 5 mg/kg/d) in patients with hereditary hemochromatosis (HH) iron overload refractory to or intolerant phlebotomy. Ten were enrolled all completed 12-month treatment period. There significant decreases from baseline end (i.e., 12 months) median serum ferritin (P < 0.001), mean transferrin saturation 0.05), liver concentration alanine aminotransferase 0.05). The time achieve reduction ≥50%...

A hemoglobina glicada, também denominada glicosilada ou glico-hemoglobina, é conhecida ainda como HbA 1C e, mais recentemente, apenas A1C.Embora seja utilizada desde 1958 uma ferramenta de diagnóstico na avaliação do controle glicêmico em pacientes diabéticos, a dosagem da A1C passou ser cada vez empregada e aceita pela comunidade científica após 1993, depois ter sido validada através dos dois estudos clínicos importantes sobre impacto as complicações crônicas diabetes mellitus (DM): o...

&lt;i&gt;Background/Aims:&lt;/i&gt; Prolonged physical exercise induces adaptive alterations in the hypothalamic-pituitary axis, increasing cortisol metabolism, and reducing synthesis glucocorticoid sensitivity. The mechanisms responsible for this relative resistance remain unknown but may involve expression of genes encoding receptor (GR) and/or inflammatory molecules nuclear factor kappa B1 (NFkB1) signaling pathway cytokines. This study aimed to determine impact prolonged training on...

This study sought to compare patients with thyroid eye disease (TED) and normal controls respect the expression of NR3C1, CHUK, IKBKB, FOS, NFKB HSD11B1 genes in orbital fat (OF) extraocular muscle (EOM).A prospective design was used evaluate 34 TED 38 healthy controls. OF harvested from 33 27 EOM biopsies were obtained 32 18 Samples examined by real-time PCR evaluated using appropriate statistical analyses a significance cut-off P < 0.05.NR3C1 mRNA levels higher (median 213 (96-376)) than...

SUMMARY Adrenal function in twenty‐three patients with paracoccidioidomycosis (South American Blastomycosis) has been assessed by measuring the response to adrenocortical stimulation 1–24 ACTH. Two overt Addison's disease showed very low basal levels and complete absence of an increase either cortisol or aldosterone secretion. Six probable diminished adrenal reserve terms three function. These findings indicate incidence significant hypoadrenalism 44% hospitalized disseminated paracoccidioidomycosis.

Abstract Background The expression of glucocorticoid-receptor (GR) seems to be a key mechanism in the regulation glucocorticoid (GC) sensitivity and is potentially involved cases GC resistance or hypersensitivity. aim this study describe method for quantitation GR alpha isoform (GRα) using real-time PCR (qrt-PCR) with analytical capabilities monitor patients, offering standard-curve reproducibility as well intra- inter-assay precision. Results Standard-curves were constructed by employing...

Glucocorticoids (GCs) are key drugs in the treatment of systemic lupus erythematosus (SLE). GC dose reduction during remission is related to disease activity, used, length treatment, and individual sensitivity. We compared receptor α (GRα) isoform nuclear factor kappaB (NF-κB) messenger RNA quantitation vivo sensitivity between SLE patients healthy controls. performed a cross-sectional study 19 women aged 22-49 years, including 9 clinical taking ≤5 mg prednisone 10 matched evaluated using 2...

Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid incubated in darkness at 25+2°C. Histological analysis callogenesis somatic indicated occurrence direct indirect development. Direct embryo formation was observed from hypocotyledonary epidermic cells only explant 18 days after inoculation. Somatic formed indirectly...

Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis as alternative improve plant production, turning economically feasible the exploitation its secondary metabolites present properties. Micropropagation by using shoot apexes produced rhizome plants were carried out on Murashige &amp; Skoog medium supplemented with...

The efficacy and safety of a 2-year treatment with deferasirox was evaluated in 31 patients sickle cell anemia transfusional iron overload. At 24 months, there were significant decreases from baseline mean serum ferritin (from 2,344.6 to 1,986.3 µg/l; p = 0.040) liver concentration 13.0 ± 5.4 9.3 5.7 mg Fe/g dry weight; &lt; 0.001). Myocardial T2* values normal (&gt;20 ms) all at did not change significantly over the course study. However, improvement left ventricular ejection fraction...

Cell suspension cultures of Bauhinia forficata Link, Curcuma zedoaria Roscoe and Phaseolus vulgaris L. were used to test plant ability utilize an alternative sucrose as carbon source energy for growth. Glycerol, sorbitol galactose the sources tested. established on liquid medium containing transferred culture supplemented with or glycerol, sorbitol, sole source. Fresh dry weight increasing protein content showed marked differences among different used. Sucrose was best all three species...

&lt;i&gt;Background/Aim:&lt;/i&gt; In several populations, major histocompatibility complex and CTLA-4 (cytotoxic T lymphocyte antigen-4) gene polymorphisms are related to adult subjects with Graves’ disease (GD). Our aim was study the association of +49A&gt;G polymorphism in Brazilian children adults GD its correlation clinical laboratory markers severity. &lt;i&gt;Methods:&lt;/i&gt; established by polymerase chain reaction-restriction fragment length analysis 44 72 compared a stringent...

PURPOSE: Establish the prevalence of Helicobacter spp in chronic cholecystitis and its correlation with gallbladder's histological findings. METHODS: 100 patients were operated for cholecystolithiasis. In pathological examination gallbladder, evaluated presence metaplasia, dysplasia, lymphoid follicles, anaplasia tumors that might be related to plus bacilli Giemsa? by optical microscopy. From DNA extracted from bile, PCR was performed using specific primers identification amplification 400bp...

We measured NF-κB, IKK, c-Fos, and GRα mRNA expression in vivo glucocorticoid sensitivity patients with rheumatoid arthritis. A very low dose intravenous dexamethasone suppression test real-time PCR quantitation of these genes were performed on blood samples from 21 arthritis who not glucocorticoids during the previous four months 20 healthy individuals. Mean duration was 8.8 years, mean disease activity, as assessed by Disease Activity Score 28 (DAS28), 4.45. Basal cortisol percentage...

A Sociedade Brasileira de Patologia Clínica/Medicina Laboratorial (SBPC/ML) lançou, em seu 44º Congresso Brasileiro Clínica e Medicina Laboratorial, uma série recomendações para minimizar os erros na fase pré-analítica, sendo este texto delas. Além do crescimento mercado testes moleculares, é frequente a percepção errônea que esses são menos sujeitos erros. Enfatizamos, neste documento, certos cuidados pré-analítica extremamente importantes garantir confiabilidade moleculares.

O diagnóstico diferencial das anemias microcíticas é complexo e sua investigação laboratorial, de custo elevado. uso índices hematimétricos para racionalizar a abordagem diagnóstica tem sido proposto contornar essa problemática. OBJETIVO: Avaliar utilidade dos nas microcíticas, modo prospectivo, em hospital geral alta complexidade. MÉTODOS: Foram analisados 2278 hemogramas realizados nos nossos serviços. Baseados eletroforese hemoglobina ferritina, estratificamos 52 pacientes adultos com...

Insulin resistance is an underlying cause of metabolic changes associated with cardiovascular diseases. Glucocorticoids are known determinant factors insulin resistance. We quantified glucocorticoid receptor alpha (GRα) mRNA and 11 beta-hydroxysteroid dehydrogenase type 1 (11β-HSD1) in various tissues 35 patients previously established disease. This was a prospective study cardiac surgery patient setting. Samples subcutaneous adipose tissue, epicardial fat, muscle, peripheral blood...

The wide variability of responses to corticotherapy suggests a role for individual recognition steroid sensitivity in order customize treatment. Oral dexamethasone (DEX) administration may be hindered by the rate its intestinal absorption and liver first-passage effect. In this study we suggest that an intravenous very low dose DEX suppression test (VLD IV-DST) can used as index glucocorticoid (GC) sensitivity.We evaluated 87 normal subjects: 44 prepubertal children, 23 adolescents 20 adults...

The activity of the hypothalamic-pituitary-adrenal axis is usually modulated by several stress factors, including exercise. Different responses are induced physical training according to duration and intensity During prolonged training, cortisol remains normal or decreased as a consequence altered secretion, metabolism excretion, possibly changes in glucocorticoid sensitivity. aim this study was evaluate impact on Eighteen cadets Air Force Academy, mean (SD) age: 18.7 (1.0) years, underwent...

Curcuma zedoaria Roscoe, planta pertencente à família Zingiberaceae que se propaga vegetativamente, tem sido utilizada amplamente por suas propriedades terapêuticas. O interesse na propagação em massa de plantas medicinais in vitro aumentado sensivelmente, pois os métodos convencionais limitam o potencial utilização destas plantas. Ápices caulinares foram inoculados meio cultura sólido contendo sais básicos e vitaminas Murashige &amp; Skoog, suplementado com 2,0 mg L-1 BAP 30 g sacarose. As...

OBJECTIVES: To analyze glucocorticoid (GC) sensitivity using intravenous very low dose dexamethasone suppression test (IV-VLD-DST) in patients with rheumatoid arthritis (RA) and its correlation receptor alpha-isoform (GRα) gene expression. METHODS: We evaluated 20 healthy controls 32 RA Health Assessment Questionnaire (HAQ) Disease Activity Score 28 joints (DAS) scores IV-VLD-DST GRα expression mononuclear cells. RESULTS: Basal cortisol the percentage of reduction after were lower than...