A5101807448- Alzheimer's disease research and treatments
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Nerve injury and regeneration
- Cancer-related gene regulation
- Planarian Biology and Electrostimulation
- Bioinformatics and Genomic Networks
- Lysosomal Storage Disorders Research
- Mass Spectrometry Techniques and Applications
- Single-cell and spatial transcriptomics
- Parkinson's Disease Mechanisms and Treatments
- Microbial Inactivation Methods
- Cellular Mechanics and Interactions
- Biochemical effects in animals
- Extracellular vesicles in disease
- Peroxisome Proliferator-Activated Receptors
- RNA Research and Splicing
- Cholinesterase and Neurodegenerative Diseases
- Neurotransmitter Receptor Influence on Behavior
- Microfluidic and Bio-sensing Technologies
- Neuroinflammation and Neurodegeneration Mechanisms
- Studies on Chitinases and Chitosanases
- Aluminum Alloys Composites Properties
- Neuroscience and Neuropharmacology Research
- Genomic variations and chromosomal abnormalities
Second Xiangya Hospital of Central South University
2023-2025
Central South University
2023-2025
Beijing Institute of Technology
2013-2024
Fudan University
2014-2024
Suzhou Municipal Hospital
2023
Nanjing Medical University
2023
Shanghai Stomatological Hospital
2022
Shenzhen Maternity and Child Healthcare Hospital
2020
Southern Medical University
2020
McLean Hospital
2015-2020
Genome-wide association studies (GWASs) have identified numerous genomic loci linked to schizophrenia (SCZ), while their pathogenic mechanisms largely remain unclear. This study demonstrated protein arginine methyltransferase 7 (PRMT7) as a key target of SCZ risk SNPs with allele-specific enhancer activity at 16q22.1. Downregulating PRMT7 in neural progenitor cells (NPCs) decreased proliferation, increased neuronal differentiation, and also led longer neurites these neurons. Conversely,...
Alzheimer's disease is pathologically defined by accumulation of extracellular amyloid-β (Aβ). Approximately 25 mutations in β-amyloid precursor protein (APP) are pathogenic and cause autosomal dominant disease. To date, the mechanism underlying effect APP mutation on Aβ generation unclear. Therefore, investigating may help understanding pathogenesis. Thus, (A673T, A673V, E682K, E693G, E693Q) were transiently co-transfected into human embryonic kidney cells. Western blot assay was used to...
Alzheimer disease (AD) is characterized by progressive memory loss, reduction in cognitive functions, and damage to the brain. The β-amyloid precursor protein can be sequentially cleaved β- secretase γ-secretase. Mutations presenilin1(PS1) are most common cause of Familial Alzheimer's ( FAD). PS1 mutations alter activity γ-secretase on cleavage protein, causing increased Aβ production. Previous studies show that βAPP-C-terminal fragment first β-scretase, primarily generating long fragments...
Alzheimer's disease (AD) is the most common neurodegenerative often associated with olfactory dysfunction. Aβ a typical AD hall marker, but Aβ-induced molecular alterations in memory remain unclear. In this study, we used 5xFAD mouse model to investigate changes. Results showed that 4-month-old have impairment accompanied by piriform cortex neuron activity decline and no sound or working impairment. addition, synapse glia functional alteration consistent across different ages at proteomic...
The China Brain Multi-omics Atlas Project (CBMAP) aims to generate a comprehensive molecular reference map of over 1,000 human brains (Phase I), spanning broad age range and multiple regions in China, address the underrepresentation East Asian populations brain research. By integrating genome, epigenome, transcriptome, proteome (including post-translational modifications), metabolome data, CBMAP is set provide rich invaluable resource for investigating underpinnings aging-related phenotypes...
The mechanisms of the transplantation neural stem cells (NSCs) in treatment Alzheimer's disease remain poorly understood. In this study, NSCs were transplanted into hippocampal CA1 region rTg (tau P301L) 4510 mouse model, a tauopathy model that is thought to reflect tau pathology associated with disease. results revealed NSC reduced abnormal aggregation tau, resulting significant improvements short-term memory mice. Compared wild-type and phosphate-buffered saline (PBS)-treated mice, mice...
Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disease caused by the deficiency of enzyme α-l-iduronidase (IDUA), typically leading to devastating secondary pathophysiological cascades. Due irreversible nature disease's progression, early diagnosis and interventional treatment has become particularly crucial. Considering fact that serum urine are most commonly used specimens in clinical practice for detection, we conducted an analysis identify differential protein profile MPS...
Abstract Single-cell/nuclei RNA sequencing (sc/snRNA-Seq) is widely used for profiling cell-type gene expressions in biomedical research. An important but underappreciated issue the quality of sc/snRNA-Seq data that would impact reliability downstream analyses. Here we evaluated precision and accuracy 18 datasets. The was assessed on from human brain studies with a total 3,483,905 cells 297 individuals, by utilizing technical replicates. sample-matched scRNA-Seq pooled-cell RNA-Seq cultured...
Microgravity severely halts the structural and functional cerebral capacity of astronauts especially affecting their brains due to stress produced by cephalic fluid shift. We employed a rat tail suspension model substantiate simulated microgravity (SM) in brain. In this study, comparative mass spectrometry was applied order demonstrate differential expression 17 specific cellular defense proteins. Gamma-enolase, peptidyl-prolyl cis-trans isomerase A, glial fibrillary acidic protein, heat...
In bottom-up proteomics, the complexity of proteome requires advanced peptide separation and/or fractionation methods to acquire an in-depth understanding protein profiles. Proposed earlier as a solution-phase ion manipulation device, liquid phase traps (LPITs) were used in front mass spectrometers accumulate target ions for improved detection sensitivity. this work, LPIT-reversed chromatography-tandem spectrometry (LPIT-RPLC-MS/MS) platform was established deep proteomics. LPIT here robust...
SLC6A3 , which encodes the primary regulator of extracellular dopamine (DA) concentration, DA transporter, has been implicated in schizophrenia (SCZ). However, details its genetic effect on risk remain largely unknown. The purpose this candidate gene study was to identify a specific activity associated with SCZ by using functional approaches. We first examined neurons isolated from case-control postmortem nigral tissue and found that average mRNA level controls only 0.37-fold cases ( P =...
The application of neural stem cell (NSC) research to neurodegenerative diseases has led promising clinical trials. Currently, NSC therapy is most for Parkinson's disease (PD). We conducted behavioral tests and immunoassays the profiling a PD model in rats assess therapeutic effects treatments. Further, using multiple sample comparison workflow, combined with 18 O‐labeled proteome mixtures, we compared differentially expressed proteins from control, PD, NSC‐treated rats. results were...