A5101827695- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Animal Nutrition and Physiology
- Mycotoxins in Agriculture and Food
- Analytical Chemistry and Chromatography
- Immune Cell Function and Interaction
- Clostridium difficile and Clostridium perfringens research
- Advanced Chemical Sensor Technologies
- Aquaculture disease management and microbiota
- Pharmaceutical and Antibiotic Environmental Impacts
- Immune cells in cancer
- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Fermentation and Sensory Analysis
- Meat and Animal Product Quality
- Natural product bioactivities and synthesis
- Alcohol Consumption and Health Effects
- Biochemical Analysis and Sensing Techniques
- Diet and metabolism studies
- Mass Spectrometry Techniques and Applications
- Immune Response and Inflammation
- Epigenetics and DNA Methylation
- Tryptophan and brain disorders
- Genetic and phenotypic traits in livestock
- Diet, Metabolism, and Disease
State Key Laboratory of Food Science and Technology
2016-2025
Jiangnan University
2016-2025
Guangdong Academy of Agricultural Sciences
2016-2025
Soochow University
2025
Second Affiliated Hospital of Soochow University
2025
First Teaching Hospital of Tianjin University of Traditional Chinese Medicine
2024
Beijing Children’s Hospital
2019-2023
Capital Medical University
2013-2023
Xinjiang Agricultural University
2023
Shanghai Jiao Tong University
2011-2023
Abstract Metabolites from intestinal microbes modulate the mucosal immune system by regulating polarization and expansion of T cells. Whether microbial metabolites influence macrophage polarization, however, is poorly understood. Here, we show that large bowel fermentation product, butyrate, facilitates M2 in vitro vivo . The supernatant butyrate-treated increased migration enhanced wound closure rate MLE-12 Butyrate attenuated inflammation mice with dextran sulfate sodium (DSS)-induced...
Urine metabolites are used in many clinical and biomedical studies but usually only for a few classic compounds. Metabolomics detects vastly more metabolic signals that may be to precisely define the health status of individuals. However, compounds remain unidentified, hampering biochemical conclusions. Here, we annotate all detected by two untargeted metabolomic assays, hydrophilic interaction chromatography (HILIC)-Q Exactive HF mass spectrometry charged surface hybrid (CSH)-Q...
Abstract The mammalian brain relies on neurochemistry to fulfill its functions. Yet, the complexity of metabolome and changes during diseases or aging remain poorly understood. Here, we generate a atlas wildtype mouse from 10 anatomical regions spanning adolescence old age. We combine data three assays structurally annotate 1,547 metabolites. Almost all metabolites significantly differ between age groups, but not by sex. A shift in sphingolipid patterns related myelin remodeling is...
Interleukin 4-induced gene-1 (IL4I1) was initially described as an early IL-4-inducible gene in B cells. IL4I1 protein can inhibit T cell proliferation by releasing its enzymatic catabolite, H2O2, and this effect is associated with transient down-regulation of CD3 receptor-zeta (TCRζ) expression. Herein, we show that contributes to the regulation macrophage programming. We found expression increased during bone marrow-derived (BMDM) differentiation, much higher primary macrophages than...
Butyric acid is a beneficial feed additive used in animal production, including poultry production. However, there are few reports on butyric as prophylactic treatment against intestinal inflammation broilers. The current study explored the effect of sodium butyrate (SB) health and gut microbiota broilers with induced by dextran sulfate (DSS) monitoring changes histopathology, leakiness indicators, inflammatory cytokines, composition. Sodium supplementation prior to DSS administration...
To evaluate the effects of different combinations probiotics on performance, egg quality, and immune response layer hens, a trial was carried out with 1,800 white feather hens Lohmann variety. The experiment conducted by using completely randomized design 9 treatments, 4 replicates, 50 in each replicate. Compared control group, group F, which added composition heat-inactivated Lactobacillus salivariusCB Bacillus subtilis to diets caused highly significant (P < 0.05) increases production,...
Unknown metabolites represent a bottleneck in untargeted metabolomics research. Ion mobility-mass spectrometry (IM-MS) facilitates lipid identification because it yields collision cross section (CCS) information that is independent from mass or lipophilicity. To date, only few CCS values are publicly available for complex lipids such as phosphatidylcholines, sphingomyelins, triacylglycerides. This scarcity of data limits the use an parameter orthogonal to mass, MS/MS, retention time. A...
The sensory impacts of two thiols, 2-methyl-3-furanthiol (MFT) and 2-furfurylthiol (FFT), in Chinese soy sauce aroma-type Baijiu (SSB), strong (STB), light (LTB) (liquor) were evaluated combined with partial least squares discriminant analysis (PLS-DA) to differentiate Baijiu. flavor dilution factors these thiols ranged from 81 6561, quantitative results showed that MFT FFT significantly more abundant (p < 0.001) SSB than STB LTB. determined odor activity values (OAVs) suggest (OAV: 34–121)...
Gut microbiota plays an important role in the regulation of food allergy. However, interactions between gut flora and immune system are not well studied. Here, we obtained ovalbumin (OVA)-sensitive BALB/c mice, combined with serum untargeted metabolomics to investigate mechanisms interactions. The results showed that 17 metabolites were downregulated, enriched aminoacyl-tRNA biosynthesis pathway, whereas indole-3-propionic acid (IPA) was increased. Six operational taxonomic units (OTUs) at...
Torularhodin, a unique carotenoid, confers beneficial effects on nonalcoholic fatty liver disease (NAFLD). However, the precise mechanism underlying its therapeutic remains unknown. Here, we report that torularhodin alleviates NAFLD in male mice by modulating gut microbiota. Additionally, transplanting fecal microbiota from torularhodin-treated to germ-free also improves NAFLD. Mechanistically, specifically enriches abundance of Akkermansia muciniphila, which promoting synthesis...
The overexpression of IRX1 gene correlates with the growth arrest in gastric cancer. Furthermore, suppresses peritoneal spreading and long distance metastasis. To explore precise mechanisms, we investigated whether restoring expression affects angiogenesis or vasculogenic mimicry (VM). Human umbilical vein endothelial cells (HUVECs) chick embryo SGC-7901 cancer were used for VM analysis. Small interfering RNA was analyzing function BDKRB2, a downstream target IRX1. As results, remarkable...
Methionine (Met) is an essential and multifunctional nutrient in vertebrate diets. It a precursor of S-adenosylmethionine (SAM), the methyl donor for DNA methylation, which has important role inflammatory responses. However, whether Met exerts anti-inflammatory effects by altering methylation macrophages unclear. In this study, was found to diminish activation mitogen-activated protein kinase signaling pathway; decrease production tumor necrosis factor-α, interleukin-6, interferon-β; enhance...
Salmonella enterica is a food-borne pathogen that poses severe threat to both poultry production and human health. Antibiotics are critical for the initial treatment of bacterial infections. However, overuse misuse antibiotics results in rapid evolution antibiotic-resistant bacteria, discovery development new declining. Therefore, understanding antibiotic resistance mechanisms developing novel control measures essential. In present study, GC-MS-based metabolomics analysis was performed...
The purpose of this study was to investigate the expression TLR4 and caveolin‐1 in monocytes among healthy volunteers as well those with type‐2 diabetes mellitus (T2DM) diabetic peripheral neuropathy (DPN). Nineteen control subjects, 18 patients T2DM, 20 DPN were enrolled. Toll‐like receptor (TLR)4, caveolin‐1, MyD88, phosphorylated IκB, plasma TNF‐α interleukin (IL)‐6 measured using real‐time polymerase chain reaction, Western blotting, enzyme‐linked immunosorbent assay. Compared other two...
Host-microbiota interactions describe a co-evolution and mutualistic symbiosis. Gut microbial communities are important for diverse host functions. However, in birds, the relationship between composition of intestinal microbiota genetic variation is not clearly understood. To dissect these interactions, Chinese yellow broiler line (genetically selected high growth rate) Huiyang Beard chickens (low were crossed, generating an F2 population. The population structures gut microbes...
Residual feed intake (RFI) is a key indicator of efficiency, critical for enhancing the economic sustainability poultry production. However, genetic and metabolic regulatory mechanisms RFI remain unclear. This study analyzed genome, liver transcriptome, metabolome, lipidome hens with low high efficiency (N = 60) from previously established divergent broiler lines (F15). Our results revealed pronounced differentiation between (LRFI) (HRFI) identified genomic signatures selection associated...