A5103909296- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Intraocular Surgery and Lenses
- FOXO transcription factor regulation
- Adrenal and Paraganglionic Tumors
- Electrocatalysts for Energy Conversion
- Endodontics and Root Canal Treatments
- Traumatic Ocular and Foreign Body Injuries
- Hormonal Regulation and Hypertension
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Myasthenia Gravis and Thymoma
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Diet and metabolism studies
- Advanced battery technologies research
- Plant-based Medicinal Research
- Cancer Genomics and Diagnostics
- Immunotherapy and Immune Responses
- Thyroid Cancer Diagnosis and Treatment
- Plant biochemistry and biosynthesis
- Traditional Chinese Medicine Analysis
Hebei Academy of Agriculture and Forestry Sciences
2024-2025
Ministry of Agriculture and Rural Affairs
2023-2025
Ningbo University
2025
Air Force Medical University
2013-2024
Tianjin Medical University General Hospital
2023-2024
Qingdao University of Science and Technology
2024
Qilu Hospital of Shandong University
2009-2024
Jiangxi Provincial Cancer Hospital
2021-2024
Agriculture and Forestry University
2023
Zhejiang A & F University
2023
Abstract N6-methyladenosine (m6A) is a reversible mRNA modification that has been shown to play important roles in various biological processes. However, the of m6A macrophages are still unknown. Here, we discover ablation Mettl3 myeloid cells promotes tumour growth and metastasis vivo. In contrast wild-type mice, Mettl3-deficient mice show increased M1/M2-like tumour-associated macrophage regulatory T cell infiltration into tumours. sequencing reveals loss METTL3 impairs YTHDF1-mediated...
Alzheimer's disease (AD) is a heterogeneous with complex clinicopathological characteristics. To date, the role of m6A RNA methylation in monocyte-derived macrophages involved progression AD unknown. In our study, we found that methyltransferase-like 3 (METTL3) deficiency improved cognitive function an amyloid beta (Aβ)-induced mouse model. The mechanistic study showed METTL3 ablation attenuated modification DNA methyltransferase 3A (Dnmt3a) mRNAs and consequently impaired YTH...
Fork-head box protein M1 (FoxM1) is a transcriptional factor which plays critical roles in cancer development and progression. However, the general regulatory mechanism of FoxM1 still limited. STMN1 microtubule-binding can inhibit assembly microtubule dimer or promote depolymerization microtubules. It was reported as major responsive paclitaxel resistance for clinical chemotherapy tumor patients. But function abnormally high level its regulation cells remain unclear. In this study, we used...
Tamoxifen resistance remains to be a huge obstacle in the treatment of hormone-dependent breast cancer, and this therefore highlights dire need explore underlying mechanisms. The epithelial-mesenchymal transition (EMT) is molecular process through which an epithelial cell transfers into mesenchymal phenotype. Roles EMT embryo development, cancer invasion metastasis have been extensively reported. Herein, we established tamoxifen-resistant MCF-7/TR cells showed that underwent driven by...
Abstract Background We aimed to examine the expression level of Nucleophosmin (NPM1) protein in colon cancer tissues and investigate potential role NPM1 regulation cell migration invasiveness. Methods Immunohistochemical assay was performed pattern 31 groups colonic carcinoma samples, including tumors, adjacent normal tissues, matched metastatic lymph nodes from same patients. Small interfering RNA technique exogenous wild type methods were used further verify function NPM1. Results...
The complement component 1, q subcomponent binding protein (C1QBP/p32/HABP1) is a ubiquitously expressed and multicompartmental cellular involved in various biological processes. In order to further understand its functions, we conducted proteomics analysis of interactome this study. An improved sample preparation mass spectrometric identification strategy was developed combining high-speed centrifugation, formaldehyde labeling, two-dimensional reverse-phase liquid chromatography. Using...
Nanoframes (NFs) with highly open structures have attracted substantial attention because of their large surface area, three-dimensional structure and good stability, which are significance to enhance the catalytic characters. However, it is still challenging develop facile efficient method construct Pt-based NFs tailored structures. Herein, a simple solvothermal was developed prepare monodisperse PtCu dodecahedral nanoframes (PtCu DNFs), where cetyltrimethylammonium chloride (CTAC) served...
Quantitative real-time PCR (qRT-PCR) is a widely applied technique for accurately assessing the expression of target genes. In practice, evaluation gene requires appropriate reference To screen reliable genes evaluating via qRT-PCR in Mythimna loreyi, notorious migratory pest across Asia, Africa, Europe, and Australia, we assessed stability 13 candidate M. loreyi using ΔCt method, BestKeeper, Normfinder, GeNorm, web-based comprehensive platform RefFinder. These include RPL10, RPL27, RPL32,...
The intestinal absorption and metabolism characteristics of the potentially beneficial polyphenol rutin were studied by measuring intracellular accumulation transport into Caco-2 cells with sensitive reliable analytical method HPLC-coupled tandem mass spectrometry. Rutin glucuronidated absorbed differently basolateral apical membranes, showed differential permeability through sides. Approximately 33% was metabolized to rutin, concentration much lower than that parent rutin. P-glycoprotein...
ABSTRACT Multifunctional polymer unimolecular micelles, which are used as templates to fabricate stable gold nanoparticles (GNPs) in one‐step without external reductant, have been designed and prepared. Amphiphilic 21‐arm star‐like block copolymers β‐cyclodextrin‐{poly(lactide)‐poly(2‐(dimethylamino) ethyl methacrylate)‐poly[oligo(2‐ethyl‐2‐oxazoline)methacrylate]} 21 [β‐CD‐(PLA‐PDMAEMA‐PEtOxMA) ] the precursors synthesized by combination of ring‐opening polymerization (ROP) activators...
Abstract Hepatic glycogen is the main source of blood glucose and controls intervals between meals in mammals. storage mammalian pups insufficient compared to their adult counterparts; however, detailed molecular mechanism poorly understood. Here, we show that, similar pattern, N6-methyladenosine (m6A) modification mRNAs gradually increases during growth mice liver. Strikingly, hepatocyte-specific Mettl3 knockout mice, loss m6A disrupts liver storage. On mechanism, mRNA Gys2, liver-specific...
Three-dimensional (3D) bimetallic PtCu alloyed nanocages (NCs) with highly open structures and large external- internal-surfaces accessible for reactants were facilely synthesized by a one-pot solvothermal method. Arginine cetyltrimethylammonium chloride (CTAC) acted as the costructure-directors stabilizing agents. The resultant NCs showed significantly enhanced catalytic activity improved stability when compared to commercial Pt/C (50 wt %) Pt black catalysts toward oxygen reduction...
Riptortus pedestris (Fabricius) (Hemiptera: Alydidae) is a major soybean pest throughout East Asia that relies on its advanced olfactory system for the perception of plant-derived volatile compounds and aggregation pheromones conspecific host plant localization. Odorant binding proteins (OBPs) facilitate transport odorant across sensillum lymph within insect system, enabling their interaction with receptors (ORs). Real-time quantitative PCR (qRT-PCR) analyses, fluorescence-based competitive...
Insect sex pheromones have been widely used in integrated pest control due to their efficiency, non-toxicity, specificity, and environmental sustainability. They are considered a key component of green management techniques. Polymerus pekinensis is phytophagous plant bug on alfalfa (Medicago sativa L.) East Asia. This study gas chromatography–electroantennogram detection (GC–EAD) chromatography–mass spectrometry (GC–MS) analyze the whole-body extracts from male female P. pekinensis. Octyl...
ABSTRACT Beyond structural support, plant root systems play crucial roles in the absorption of water and nutrients, fertiliser efficiency crop yield. However, molecular mechanism regulating architecture rice remains largely unknown. In this study, a short‐root mutant was identified named Oscyp22 . showed impairment growth primary, adventitious lateral roots. Histochemical fluorescent staining analyses revealed reduced cell elongation division activity Further analysis that displayed an...
Background: Depletion of B cells has resulted in major therapeutic benefits hematological malignancies. Several FDA-approved cell-depleting therapies are available, including CAR-T and T cell engagers (TCE). Blinatumomab, the only CD19-targeting bispecific engager approved for malignancies, disadvantages clinical setting sub-optimal pharmacokinetics, neurotoxicity, CRS, lack long-term efficacy. In this study we present CC312, a trispecific antibody engineered better efficacy by leveraging...
Abstract Background: B cell depletion therapy (BCDT), such as monoclonal antibodies, bispecific T engagers(TCE) and CAR-T,has an extensive history of effectively treating malignancies. Certain BCDT antibodies,e.g. rituximab,are also approved to treat autoimmune diseases(ADs), however demonstrate poor cells in deep tissue which may limit the long-term disease control or remission patients. Recently, anti-CD19 CAR-T, through thorough “resetting” populations, have achieved multi-year drug-free...