Vaspin was identified as an adipokine with insulin-sensitizing effects, which is predominantly secreted from visceral adipose tissue in a rat model of type 2 diabetes. We have recently shown that vaspin mRNA expression related to parameters obesity and glucose metabolism. However, the regulation serum concentrations human diabetes unknown.For measurement concentrations, we developed enzyme-linked immunosorbent assay (ELISA). Using this ELISA, assessed circulating cross-sectional study 187...

The dysregulation of adipokines is closely associated with the pathogenesis insulin resistance and type 2 diabetes. Retinol-binding protein-4 (RBP4), a new adipokine, was recently reported to provide link between obesity resistance. Here, we examined relation plasma RBP4 concentrations various metabolic parameters in humans.An enzyme-linked immunosorbent assay developed measure human concentrations, which were then compared related subjects normal glucose tolerance (NGT; n = 57), impaired...

Resistin is an adipocyte-derived peptide that might play a role in obesity and insulin resistance. However, its humans largely unclear. Although many studies have measured the expression of human resistin tissues, circulating concentrations relation to metabolic parameters are unknown. We developed ELISA for plasma aged individuals with or without type 2 diabetes mellitus. To validate results our subjects, adiponectin were also determined, which higher nondiabetic subjects than diabetic...

OBJECTIVE—Progranulin is an important molecule in inflammatory response. Chronic inflammation frequently associated with central obesity and disturbances; however, the role of circulating progranulin human obesity, type 2 diabetes, dyslipidemia unknown. RESEARCH DESIGN AND METHODS—For measurement serum concentrations, we developed enzyme-linked immunosorbent assay (ELISA). Using this ELISA, assessed a cross-sectional study 209 subjects wide range body fat distribution, insulin sensitivity,...

Retinol binding protein 4 (RBP4) is a useful biomarker in the diagnosis of type 2 diabetes since its level serum higher insulin-resistant states. Accurate measurement RBP4 levels hampered by conventional immunologic methods, such as enzyme-linked immunosorbent assay (ELISA). In this study, therefore, we have developed an aptamer-based surface plasmon resonance (SPR) biosensor that can be used to sense for samples. A single-stranded DNA (ssDNA) aptamer showed high affinity (Kd = 0.2 +/- 0.03...

In obesity, elevated fat mass and ectopic accumulation are associated with changes in adipokine secretion, which may link obesity to inflammation the development of insulin resistance. However, relationships among individual adipokines between parameters glucose metabolism or largely unknown. Serum concentrations 20 were measured 141 Caucasian obese men (n = 67) women 74) a wide range body weight, glycemia sensitivity. Unbiased, distance-based hierarchical cluster analyses performed...

OBJECTIVE The angiopoietin-like protein 4 (Angptl4)/fasting-induced adipose factor (Fiaf) is known as a regulator of peripheral lipid and glucose metabolism. In the present study, we investigated physiological role Angptl4 in central regulation body weight homeostasis. RESEARCH DESIGN AND METHODS Hypothalamic expression levels were measured using immunoblot assay during feeding manipulation or after administration leptin, insulin, nutrients. effects on food intake, weight, energy expenditure...

Recent studies have suggested that a novel adipokine, C1q/tumor necrosis factor-related protein-3 (CTRP-3), paralog of adiponectin, may play an important role in the regulation glucose metabolism and innate immunity. Pigment epithelium-derived factor (PEDF), multifunctional protein with antioxidant anti-inflammatory properties, is associated insulin resistance metabolic syndrome. We examined circulating CTRP-3 PEDF concentrations 345 subjects diverse tolerance statuses. Furthermore, we...

Objective Progranulin and C1q/TNF-related protein-3 (CTRP3) were recently discovered as novel adipokines which may link obesity with altered regulation of glucose metabolism, chronic inflammation insulin resistance. Research Design Methods We examined circulating progranulin CTRP3 concentrations in 127 subjects (n = 44) or without metabolic syndrome 83). Furthermore, we evaluated the relationship levels inflammatory markers cardiometabolic risk factors, including high-sensitivity C-reactive...

Obesity and insulin resistance are hallmarks of the metabolic syndrome, which is associated with low-grade chronic inflammation. Clusterin/apolipoprotein J an abundant plasma chaperone protein that has recently been suggested as a potential biomarker reflects inflammatory process in Alzheimer's disease. In present study, we investigated anthropometric clinical factors affecting levels clusterin healthy Korean subjects. We measured fasting adults (111 men 93 women) using ELISA kit. analyzed...

Cardiomyocyte apoptosis is an important remodeling event contributing to heart failure and adiponectin may mediate cardioprotective effects at least in part via attenuating apoptosis. Here we used hypoxia-reoxygenation (H/R) induced H9c2 cells examine the effect of cellular mechanisms action. We first TUNEL labeling combination with laser scanning cytometry demonstrate that prevented H/R-induced DNA fragmentation. The anti-apoptotic was also verified attenuation phosphatidylserine exposure...

Irisin is an exercise-induced novel myokine that drives brown-fat-like conversion of white adipose tissue and has been suggested to be a promising target for the treatment obesity-related metabolic disorders. To assess association circulating irisin concentrations with brown (BAT) and/or sarcopenia in humans. We examined levels 40 BAT-positive BAT-negative women detected by 18F-fluorodeoxyglucose positron emission tomography (18FDG-PET). In separate study, we also 401 subjects or without...

Adiponectin (APN) is a crucial regulator for many inflammatory processes, but its effect on Th cell-mediated responses has not been fully understood. Thus, we investigated the immune-modulatory effects of APN dendritic cells (DCs) controlling cell polarization. induced maturation and activation DCs, as demonstrated by increased expression MHC class II, costimulatory molecules in both mouse human it significantly enhanced production proinflammatory cytokines. triggered degradation IκB...

A new member of human beta-chemokine cDNA was isolated and named leukotactin-1 (Lkn-1). Lkn-1, along with murine macrophage inflammatory protein-related protein-1 -2, defines a subgroup beta-chemokines based on two conserved cysteines in addition to the four others all beta-chemokines. The putative mature Lkn-1 is composed 92 amino acids calculated m.w. 10,162. gene mapped chromosome 17, region q12. Recombinant potent chemoattractant for neutrophils, monocytes, lymphocytes induced calcium...

Summary Objective Here we use a novel ELISA that is specific for full‐length visfatin (PBEF/NAMPT), compare it with the existing C‐terminal based assay and to investigate associations of metabolic parameters. Design, patients measurements We established specificity effectiveness new evaluated clinical, anthropometric parameters in cross‐sectional study 129 Thai subjects, consisting 50 outpatients type 2 diabetes 79 healthy volunteers. Results The accurately recovered recombinant detected...

C1q/TNF-related protein-3 (CTRP-3), an adiponectin paralog, and progranulin were recently identified as novel adipokines which may link obesity with glucose dysregulation subclinical inflammation. We analyzed the relationship between CTRP-3, coronary artery disease (CAD) in Korean men women. Circulating CTRP-3 levels examined 362 adults acute syndrome (ACS, n = 69), stable angina pectoris (SAP, 85), control subjects (n 208) along various kinds of cardiometabolic risk factors. concentrations...

The aims of this study were to investigate whether circulating irisin is associated with favorable metabolic parameters and how the association differs according body composition in humans. A total 424 subjects (233 men 191 women), aged 23–73 years (mean age 47.1 years), enrolled from Seoul Metro City Diabetes Prevention Program. Body was determined using an impedance analyzer, serum level measured a commercial kit. Serum correlated including less obese, lower blood pressure glucose levels...

Abstract Aims To investigate any association between Type 2 diabetes mellitus and two single nucleotide polymorphisms (SNPs) in the adiponectin gene, T45G G276T, Korean population. Methods We genotyped 427 non‐diabetic controls 493 diabetic patients for SNPs G276T of measured plasma concentrations, examined clinical parameters Koreans. Results There were no statistically significant differences allele frequencies 45 276 comparing control with subjects (T frequency 68.3% vs. 71.6%, P = 0.13...

Women with previous gestational diabetes mellitus (pGDM) are at high risk of developing type 2 in the future. The role adipokines women pGDM has not been established.We investigated whether circulating adipokine concentration is associated abnormal glucose homeostasis pGDM. DESIGN, SETTING, PATIENTS, AND MAIN OUTCOME MEASURES: We measured plasma concentrations retinol-binding protein-4 (RBP4), transthyretin (TTR), and adiponectin metabolic parameters four groups who exhibited normal...

Visceral adipose tissue-derived serine protease inhibitor (vaspin) is a novel adipokine with insulin-sensitizing effects. However, the physiological role for vaspin in human metabolic regulation remains to be established.We studied 24-h profiles of circulating concentrations relation meal ingestion normal adults.Blood samples were drawn 39 times throughout period from 10 healthy male subjects provided meals on fixed schedule. On separate day, four fasted and then an unexpected clarify effect...