A5100756609- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Nutrition and Physiology
- Plant Virus Research Studies
- Rabbits: Nutrition, Reproduction, Health
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Immunology Research
- Moringa oleifera research and applications
- Phytase and its Applications
- Animal Behavior and Welfare Studies
- Ruminant Nutrition and Digestive Physiology
- Selenium in Biological Systems
- Microbial infections and disease research
- Effects of Environmental Stressors on Livestock
- Myasthenia Gravis and Thymoma
- Lipid metabolism and biosynthesis
- Herpesvirus Infections and Treatments
- Liver Disease Diagnosis and Treatment
- Parkinson's Disease and Spinal Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics, phytochemicals, and oxidative stress
- Cancer Treatment and Pharmacology
- Livestock and Poultry Management
- Pharmacological Effects and Assays
Harbin Veterinary Research Institute
2010-2024
Chinese Academy of Agricultural Sciences
2010-2024
Baoding No.1 Central Hospital
2019
Baoding People's Hospital
2019
Northwest A&F University
2010
Northeast Agricultural University
2010
Yunnan Agricultural University
2007
University of Manitoba
1998
Danish Cattle Research Centre
1995
Schothorst Feed Research
1994
Porcine reproductive and respiratory syndrome virus (PRRSV) infection induces both humoral cellular immune responses. In this study, we investigated the changes in cytokine levels peripheral blood between highly pathogenic PRRSV HuN4 strain its derivative HuN4-F112 obtained by serial propagation MARC145 cells to 112 passages. The results demonstrated that pigs infected with showed a loss of appetite, decrease body weight, raised temperature, symptoms, along interstitial pneumonia lesions....
In China, porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are widely used. These vaccines, which contain inactivated live attenuated (LAVs), produced by MARC-145 cells derived from the monkey kidney cell line. However, some PRRSV strains in have a low yield. Here, we used two type 2 (CH-1R HuN4) to identify genes responsible for yield cells. Our findings indicate that viruses different spread patterns, ultimately determine their By replacing viral envelope with reverse...
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) Streptococcus suis are common pathogens in pigs. In samples collected during the porcine high fever (PHFS) outbreak many parts of China, PRRSV S. serotype 7 (SS7) have always been isolated together. To determine whether PRRSV-SS7 coinfection was cause PHFS outbreak, we evaluated pathogenicity and/or SS7 a pig model single mixed infection. Results Respiratory disease, diarrhea, anorexia were observed all infected...
Previously, we demonstrated that the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) HuN4 strain causes obvious thymic atrophy thymocytes apoptosis in infected piglets after birth, which is more severe than induced by classical PRRSV. In this study, investigated autophagy thymus of with HP-PRRSV strain, found both occurred HP-PRRSV. addition to a few virus-infected cells, CD14+ main autophagic cells were epithelial cells. These findings induces bystander...
Abstract Background Our previous studies have demonstrated that piglets infected with highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) may develop significant thymus atrophy, which related to thymocytes apoptosis. However, apart from detected in the thymus, there are no reports describing cell apoptosis induced by HP-PRRSV infection. In this study, we analyzed comparatively pathological changes, viral load peripheral immune organs including tonsil, inguinal...
Previous studies with broiler have shown dietary supplementation multi-enzyme complex containing non-starch polysaccharides (NSP) degrading enzymes and phytase is efficient in releasing phosphorus (P), calcium (Ca), energy amino acids from corn-soybean meal diets or corn-sorghum diets, hence compensating considerable levels of nutrients formulation. Notwithstanding, such potentials not been well defined duck nutrition. Giving China being the largest producing country, we conducted this study...
// Tong-Yun Wang 1, * , Yong-Gang Liu Liang Li 1 Gang Hai-Ming Hong-Liang Zhang Shi-Fei Zhao Jia-Cong Gao Tong-Qing An Zhi-Jun Tian Yan-Dong Tang and Xue-Hui Cai State Key Laboratory of Veterinary Biotechnology, Harbin Research Institute Chinese Academy Agricultural Sciences, 150001, China These authors contributed equally to this work Correspondence to: Cai, email: cai139@hvri.ac.cn Tang, tangyandong2008@163.com Keywords: PRRSV; CD163; infection; switch; abundance Received: July 07,...
Chronic heat stress (CHS) induces metabolic changes in skeletal muscle from growth to maintenance that jeopardizes performance, carcass traits, and meat quality of pigs. We investigated the protective effect dietary organic selenium (hydroxy-4-methylselenobutanoic acid, OH-SeMet) on CHS-induced damages growing pigs, corresponding responses selenoproteins. A total 40 ((Landrace ×Yorkshire) × Duroc) pigs with an average live weight 49.64 ± 2.48 kg were used this 4-week trial. Pigs randomly...
Highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) has been shown to have a wide range of tissue tropism, can directly indirectly induce cellular apoptosis. However, the impact HP-PRRSV infection on bone marrow (BM) piglets remains unclear. In this study, we investigated BM as novel site by strain in piglets. infected SWC3+SWC8- cells induced undergo The number apoptotic highlights striking effects central immune organs (BM thymus) that may enhance...
As one of many nonstructural proteins porcine reproductive and respiratory syndrome virus (PRRSV), protein 12 (Nsp12) has received relatively little attention, its role in replication, if any, is essentially unknown. By the application reverse genetic manipulation an infectious PRRSV clone, current study first to demonstrate that Nsp12 a key component replication. In addition, biochemical properties were evaluated, revealing forms dimers when exposed oxidative conditions. Furthermore, we...
This study used 40 castrated male pigs to determine the protective effects of a new selenium molecule (hydroxy selenomethionine, OH-SeMet) on dietary oxidative stress (DOS) induced hepatic lipid metabolism disorder, and corresponding response selenotranscriptome. The were randomly grouped into 5 treatments fed basal diet formulated with either normal corn oils or oxidized in which replaced by aged oils, supplemented OH-SeMet at 0.0, 0.3, 0.6 0.9 mg Se/kg for period 16 weeks (n = 8). results...
Early detection of porcine reproductive and respiratory syndrome virus (PRRSV) infection swine is necessary to control this devastating disease. By monitoring host serum antibodies viral antigens, early within herds feasible. In study, recombinant antigens were generated using DNA techniques fuse PRRSV structural protein (N) or nonstructural 1α (nsp1α) with the Rellina luciferase gene. Next, fused genes cloned into plasmids transfected HEK-293 T cells for transient expression. Upon...
The inability of current vaccines to provide effective protection against porcine reproductive and respiratory syndrome virus (PRRSV) infection is not fully understood. One the reasons might be presence anti-idiotypic antibodies (Ab2s) envelope glycoprotein GP5 induced by PRRSV since our previous studies demonstrated auto-Ab2s (aAb2s) in pigs infected with PRRSV. To test this hypothesis, negative piglets were injected a monoclonal Ab2 (Mab2-5G2) aAb2s that are specific for anti-GP5 antibody,...