A5085834756- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Gut microbiota and health
- Immunotherapy and Immune Responses
- interferon and immune responses
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Cytomegalovirus and herpesvirus research
- Helicobacter pylori-related gastroenterology studies
- Probiotics and Fermented Foods
- Viral Infections and Immunology Research
- vaccines and immunoinformatics approaches
- Escherichia coli research studies
- Liver Disease Diagnosis and Treatment
- Air Quality and Health Impacts
- Viral Infections and Vectors
- Single-cell and spatial transcriptomics
- Diabetes and associated disorders
- Eosinophilic Esophagitis
- RNA regulation and disease
- Sphingolipid Metabolism and Signaling
Emory University
2012-2025
Washington University in St. Louis
2012-2023
Howard Hughes Medical Institute
2014
General Department of Preventive Medicine
2014
Kantonsspital St. Gallen
2006-2013
University of St. Gallen
2010-2012
Mexican Social Security Institute
2005-2011
Centro Medico Nacional Siglo XXI
2007-2010
Hospital de Especialidades
2006-2010
Universidad Nacional Autónoma de México
2009
Tolerogenic T cells need probiotics CD4 + CD8αα double-positive intraepithelial lymphocytes (DP IELs) are a recently discovered class of intestinal believed to take part in variety immune responses, including oral tolerance. These absent germ-free mice, but the mechanisms driving their development unclear. Cervantes-Barragan et al. found that particular species probiotic bacteria, Lactobacillus reuteri , induces DP IELs. This does not occur by stimulating system directly. Instead, L....
Coronaviruses are of veterinary and medical importance include highly pathogenic zoonotic viruses, such as SARS-CoV MERS-CoV. They known to efficiently evade early innate immune responses, manifesting in almost negligible expression type-I interferons (IFN-I). This evasion strategy suggests an evolutionary conserved viral function that has evolved prevent RNA-based sensing infection vertebrate hosts. Here we show the coronavirus endonuclease (EndoU) activity is key induction double-stranded...
Regulatory T cells (T regs ) accumulate in the visceral adipose tissue (VAT) to maintain systemic metabolic homeostasis but decline during obesity. Here, we explored pathways controlling homeostasis, composition, and function of VAT under normal high-fat diet feeding conditions. We found that cholesterol metabolism was specifically up-regulated ST2 hi reg subsets. -specific deletion Srebf2 , master regulator selectively reduced increasing inflammation insulin resistance. Single-cell RNA/T...
Attenuated viral vaccines can be generated by targeting essential pathogenicity factors. We report here the rational design of an attenuated recombinant coronavirus vaccine based on a deletion in coding sequence non-structural protein 1 (nsp1). In cell culture, nsp1 mouse hepatitis virus (MHV), like its SARS-coronavirus homolog, strongly reduced cellular gene expression. The effect MHV replication vitro and vivo was analyzed using encoding nsp1-coding sequence. mutant grew normally tissue...
Infections with persistent viruses are a frequent cause of immunosuppression, autoimmune sequelae, and/or neoplastic disease. Plasmacytoid dendritic cells (pDCs) innate immune that produce type I interferon (IFN-I) and other cytokines in response to virus-derived nucleic acids. Persistent often depletion or functional impairment pDCs, but the role pDCs control these remains unclear. We used conditional targeting pDC-specific transcription factor E2-2 generate mice constitutively lack...
ABSTRACT Viral infection of the liver can lead to severe tissue damage when high levels viral replication and spread in organ are coupled with strong induction inflammatory responses. Here we report an unexpected correlation between expression a functional X domain encoded by hepatotropic mouse hepatitis virus strain A59 (MHV-A59), high-level production cytokines, acute mice. X-domain (also called macro domain) proteins possess poly-ADP-ribose binding and/or ADP-ribose-1′′-phosphatase (ADRP)...
Abstract The swift production of type I IFNs is one the fundamental aspects innate immune responses against viruses. Plasmacytoid dendritic cell-derived are prime importance for initial control highly cytopathic viruses such as mouse hepatitis virus (MHV). aim this study was to determine major target cell populations first wave IFNs. Generation bone marrow-chimeric mice expressing IFN receptor (IFNAR) on either hemopoietic or non-bone marrow-derived cells revealed that early MHV depended...
The innate immune response plays an essential role in the prevention of early viral dissemination. We used lymphocytic choriomeningitis virus model system to analyze tissue macrophages/Kupffer cells this process. Our findings demonstrated that Kupffer are for efficient capture infectious and preventing replication. latter process involved activation by interferon (IFN)-I prevented spread neighboring hepatocytes. In absence cells, hepatocytes were not able suppress replication, even presence...
Retention of lymphocytes in the intestinal mucosa requires specialized chemokine receptors and adhesion molecules. We find that both CD4+CD8+ CD4+ T cells epithelium, as well CD8+ mesenteric lymph nodes, express cell molecule class I–restricted cell–associated (Crtam) upon activation, whereas ligand Crtam, 1 (Cadm1), is expressed on gut CD103+DCs. Lack Crtam–Cadm1 interactions Crtam−/− Cadm1−/− mice results loss cells, which arise from mucosal acquire a CD8 lineage expression profile. After...
We examined the ability of porins from Salmonella enterica serovar typhi to induce a long-term antibody response in BALB/c mice. These triggered strong lifelong production immunoglobulin G (IgG) absence exogenous adjuvant. Analysis IgG subclasses produced during this revealed presence IgG2b, IgG1, IgG2a and weak IgG3. Despite high homology porins, long-lasting anti-S. porin sera did not cross-react with S. typhimurium. Notably, antiporin showed sustained bactericidal-binding activity...
Abstract TLR directly induce innate immune responses by sensing a variety of microbial components and are critical for the fine‐tuning subsequent adaptive responses. However, their impact mechanism action on antibody against bacterial antigens not yet fully understood. Salmonella enterica serovar Typhi ( S. typhi ) porins have been characterized as inducers long‐lasting specific in mice. In this report, we show that immunization TLR4‐deficient (TLR4 −/− ), myeloid differentiating gene...
Efficient vaccination against infectious agents and tumors depends on specific antigen targeting to dendritic cells (DCs). We report here that biosafe coronavirus-based vaccine vectors facilitate delivery of multiple antigens immunostimulatory cytokines professional antigen-presenting in vitro vivo. Vaccine based heavily attenuated murine coronavirus genomes were generated express epitopes from the lymphocytic choriomeningitis virus glycoprotein, or human Melan-A, combination with cytokine...
Regulatory T cells (Tregs) are important for the attenuation of immune reactions. During viral CNS infections, however, an indiscriminate maintenance privilege through Treg-mediated negative regulation could prevent autoimmune sequelae but impair control replication. We analyzed in this study impact Tregs on development acute encephalomyelitis, cell-mediated antiviral protection, and prevention autoimmunity following intranasal infection with gliatropic mouse hepatitis virus strain A59. To...
Abstract Lactobacilli are prevalent members of the intestinal and reproductive tract microbiota humans other species. They commonly used in probiotics various food products due to their beneficial effects on human health. For example, these microbes treat diarrhea caused by antibiotic therapy given during treatment. Despite many studies conducted understand Lactobacilli, less is known about resistance heteroresistance antibiotics. In this study, we evaluated heterogeneity eight Lactobacillus...
Summary Identifying the properties of a molecule involved in efficient activation innate and adaptive immune responses that lead to long‐lasting immunity is crucial for vaccine adjuvant development. Here we show papaya mosaic virus (PapMV) recognized by system as pathogen‐associated molecular pattern (PAMP) an antigen mice (Pamptigen). A single immunization PapMV without added efficiently induced both cellular specific antibody responses. also activated responses, shown induction lipid raft...