A5103133116- Cancer Genomics and Diagnostics
- Ovarian cancer diagnosis and treatment
- Cancer-related molecular mechanisms research
- Ruminant Nutrition and Digestive Physiology
- MicroRNA in disease regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Digital Imaging for Blood Diseases
- Eosinophilic Disorders and Syndromes
- Biosensors and Analytical Detection
- Protein Hydrolysis and Bioactive Peptides
- Atherosclerosis and Cardiovascular Diseases
- Pediatric Urology and Nephrology Studies
- Vitamin D Research Studies
- Vitamin C and Antioxidants Research
- Liver Disease Diagnosis and Treatment
- Agricultural Systems and Practices
- Liver Diseases and Immunity
- Stress Responses and Cortisol
- Inflammatory Biomarkers in Disease Prognosis
- AI in cancer detection
- Animal Genetics and Reproduction
- Immune Cell Function and Interaction
- Hepatocellular Carcinoma Treatment and Prognosis
- Urinary Tract Infections Management
- Hepatitis B Virus Studies
Sun Yat-sen University
2010-2024
The First Affiliated Hospital, Sun Yat-sen University
2010-2024
Wuhan Puai Hospital
2023
Yangzhou University
2017-2020
The First People's Hospital of Tianmen
2016
The University of Texas MD Anderson Cancer Center
2014
Ageing is often accompanied with a decline in immune system function, resulting ageing. Numerous studies have focussed on the changes different lymphocyte subsets diseases and immunosenescence. The change phenotype key indication of diseased or healthy status. However, number brought about by ageing not been comprehensively analysed. Here, we analysed T natural killer (NK) cell subsets, differentiation states 43,096 individuals, aged 20-88 years, without known diseases. Thirty-six parameters...
Introduction. Our objective was to evaluate a newly invented urine flow cytometer, and select an optimal strategy for urinalysis in clinical practice. Methods. The performance of UF-1000i evaluated both control material patient samples. A total 1631 specimens were collected analysed by visual microscopy examination (VME), cytometer (Sysmex Medical Electronics Co, Kobe, Japan) automated dipstick reflectometer Clinitek Atlas (Bayer Corp, Elkhart, USA). Results. showed good imprecision the main...
The study aimed to evaluate the effectiveness of learning blood cell morphology by on our Artificial intelligence (AI)-based online platform.Our is based mixed-methods sequential explanatory design and crossover design. Thirty-one third-year medical students were randomly divided into two groups. groups had platform microscopy in diferent sequences with pretests posttests, respectively. Students interviewed, records coded analyzed NVivo 12.0.For both groups, test scores increased...
Recent technological advances offer an opportunity to further elucidate the complex cytokine network in Major Depressive Disorder (MDD). The objective of this study was investigate role pro-inflammatory and anti-inflammatory cytokines mechanism depressive disorders. Given activating on hypothalamic-pituitary-adrenal (HPA) axis, relevance its regulation MDD, we also analyzed relationships between several cortisol levels.Twenty-five unipolar patients 20 healthy controls were recruited study....
This study aimed to develop and validate a nomogram for overall survival (OS) prediction in which combine clinical characteristics hematological biomarkers patients with hepatocellular carcinoma (HCC). We performed retrospective analysis of 807 HCC patients. All the data these were collected through electronic medical record (EMR). The independent predictive variables identified by cox regression analysis. tested accuracy nomograms discrimination calibration, then plotted decision curves...
We developed an artificial intelligence (AI) model that evaluates the feasibility of AI-assisted multiparameter flow cytometry (MFC) diagnosis acute leukemia. Two hundred leukemia patients and 94 with cytopenia(s) or hematocytosis were selected to study AI application in MFC The kappa test analyzed consistency diagnostic results immunophenotype Bland-Altman Pearson analyses evaluated correlation abnormal cell proportion between manual methods. analysis time for each case (83.72 ± 23.90 s,...
Abstract Background Non‐anemic thalassemia trait (TT) accounted for a high proportion of TT cases in South China. Objective To use artificial intelligence (AI) analysis erythrocyte morphology and machine learning (ML) to identify gene carriers non‐anemic population. Methods Digital morphological data from 76 97 controls were collected. The AI technology‐based Mindray MC‐100i was used quantitatively analyze the percentage abnormal erythrocytes. Further, ML construct prediction model. Results...
NOP2/Sun domain family member 2 (NSUN2) is upregulated in numerous types of tumors and may be implicated multiple biological processes, including cell proliferation, migration human tumorigenesis. However, little known about how NSUN2 serves a role these processes. In the present study, expression profiles long noncoding RNAs (lncRNAs) mRNAs were developed NSUN2‑deficient HepG2 cells by RNA‑sequencing analysis. A total 757 lncRNAs differentially expressed, 392 which upregulated, 365...
Background: Compared with the proven utility of flow cytometry immunophenotyping (FCI) analysis in workup myelodysplastic syndromes (MDS), immunophenotypic alterations myeloproliferative neoplasms (MPN) have been less studied and potential FCI is not defined. Methods: Bone marrow (BM) samples 83 Philadelphia-negative MPN patients were assessed by multicolor including 27 essential thrombocythemia (ET); 17 polycythemia vera (PV); 33 primary myelofibrosis (PMF) 6 MPN-unclassifiable (MPN-U). The...
Period2 (PER2), a core clock gene, encodes circadian rhythm protein which has been shown to control mammary metabolism in rodents. Whether PER2 regulates milk component synthesis such as αs–casein bovine cells is unknown. Thus, we used gene silencing technology determine if could affect and cell growth cultured primary epithelial (BMEC). The BMEC were established by enzymatic digestion of tissue from mid-lactation cows. A transient-transfection technique was insert small interfering RNA...
It was discovered that the somatic mutation in JAK2 exon 14 (JAK2V617F) totally modified understanding and diagnosis of Philadelphia-Negative myeloproliferative neoplasm (Ph-MPNs), including polycythemia vera (PV), essential thrombocythemia (ET), primary myelofibrosis (PMF). Real-time quantitative PCR is most widely used method for JAK2V617F detection clinical laboratory. In this study, we aimed to evaluate significance allele burden Ph-MPNs detected by real-time PCR.A total 208 bone marrow...
The effects of rice straw silage with combined additives (RSSA) Lactobacillus plantarum, Trichoderma viride and wheat bran on growth performance, digestibility rumen fermentation in growing lambs were investigated. Twelve initial body weight (BW ± SD) 12.8 0.4 kg selected randomly divided into 2 groups. These fed (25%, the dry matter intake (DMI) without (RSSW) 25% RSSA supplementation respectively. nutrients parameters determined. results this study showed that average daily gain (ADG) feed...
This experiment was conducted to investigate the effects of certain long-chain fatty acid combinations on ruminal microbial community in vitro. Three cows fitted with permanent cannulas were used provide rumen liquor for vitro trail. The treatments stearic acid, oleic linoleic and linolenic which respectively group A (calcium palmitate, control), B (1.5%, 1.0%, 0.5% 1.5%, acetic type fermentation), C 1.5% propionic D (1.0%, 0.5%, butyric fermentation). goats cannula culture medium collected...
Abstract To evaluate whether low coverage whole genome sequencing is suitable for the detection of malignant pelvic mass and compare its diagnostic value with traditional tumor markers. We enrolled 63 patients a suspicious ovarian malignancy. Each patient underwent (LCWGS) markers test. The masses were finally confirmed via pathological examination. copy number variants (CNVs) detected Stouffers Z-scores each CNV was extracted. risk malignancy (RM) sample calculated based on counts Z-scores,...
Objective To investigate the clinical diagnosis value of serum glycocholic acid, total bile acid(TBA), alanine transarninase(ALT) and thyroid stimulating hormone(TSH) levels in intrahepatic cholestasis pregnancy(ICP). Methods From January to December 2015, a 62 cases ICP pregnant women(included group) 1 160 healthy women at same time outpatient for antenatal examination(included control Tianmen First People′s Hospital Hubei, were chosen as study objects.Detection TBA, ALT TSH all...