A5100703466- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- GABA and Rice Research
- Food composition and properties
- Plant Reproductive Biology
- Plant nutrient uptake and metabolism
- Biofuel production and bioconversion
- Rice Cultivation and Yield Improvement
- Cellular transport and secretion
- Plant Stress Responses and Tolerance
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
- Genetic Mapping and Diversity in Plants and Animals
- Plant Genetic and Mutation Studies
- Fungal and yeast genetics research
- Lipid metabolism and biosynthesis
- Phytase and its Applications
- Plant responses to water stress
- Reproductive Physiology in Livestock
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Plant-Microbe Interactions and Immunity
Nanjing Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2025
Beijing University of Technology
2024
Jiangxi Agricultural University
2022-2024
Zhongshan Hospital
2024
Lanzhou University
2022
State Key Laboratory of Surface Physics
2021
University of Jinan
2021
Fudan University
2021
Beijing Forestry University
2016-2018
Starch is the most widespread form of energy storage in plant kingdom. Although many enzymes and related factors have been identified for starch biosynthesis, unknown players remain to be identified, given that it a complicated sophisticated process. The endosperm rice (Oryza sativa) has used study synthesis. Here, we report cloning characterization FLOURY ENDOSPERM6 (FLO6) gene rice. In flo6 mutant, content decreased normal physicochemical features are changed. Significantly, mutant cells...
Abstract Traits such as grain shape, panicle length and seed shattering, play important roles in yield harvest. In this study, the cloning functional analysis of PANICLE TRAITS 2 ( PT2 ), a novel gene from Indica rice Chuandali (CDL), is reported. synonymous with Growth‐Regulating Factor 4 OsGRF4 which encodes growth‐regulating factor that positively regulates shape negatively shattering. Higher expression correlated larger grain, longer lower A unique mutation, occurs at OsmiRNA396 target...
Rice glutelins are synthesized at the endoplasmic reticulum (ER) as precursors (pro-glutelins), and transported to protein storage vacuoles, where they processed into mature proteins. The molecular basis of this process is largely unknown. Here, we report isolation a rice mutant, gpa1, that accumulates 57 kDa pro-glutelins in seeds whose endosperm has floury appearance. Transmission electron microscopy analysis showed gpa1 cells have an enlarged ER lumen smaller body II (PBII), accumulated...
Abstract In seed plants, a major pathway for sorting of storage proteins to the protein vacuole (PSV) depends on Golgi-derived dense vesicles (DVs). However, molecular mechanisms regulating directional trafficking DVs PSVs remain largely elusive. Here, we report functional characterization rice (Oryza sativa) glutelin precursor accumulation3 (gpa3) mutant, which exhibits floury endosperm phenotype and accumulates excess proglutelins in dry seeds. Cytological immunocytochemistry studies...
Tillering and panicle branching are important determinants of plant architecture yield potential in rice (Oryza sativa). IDEAL PLANT ARCHITECTURE1 (IPA1) encodesSQUAMOSA PROMOTER BINDING PROTEIN-LIKE14, which acts as a key transcription factor regulating tiller outgrowth by directly activating the expression O. sativa TEOSINTE BRANCHED1 (OsTB1) DENSE AND ERECT PANICLE1 (OsDEP1), thereby influencing grain rice. Here, we report identification mutant named shi1 that is characterized...
In cereal crops, starch synthesis and storage depend mainly on a specialized class of plastids, termed amyloplasts. Despite the importance starch, molecular machinery regulating amyloplast development remains largely unknown. Here, we report characterization rice (Oryza sativa) floury endosperm7 (flo7) mutant, which develops floury-white endosperm only in periphery not inner portion. Consistent with phenotypic alternation flo7 endosperm, mutant had reduced amylose content seriously disrupted...
Abstract Bamboo is now widely used in construction, papermaking, textile, furniture and other fields because of its renewable, fast-growing, high-strength, high-yield easy processing. However, compared with wood, bamboo products are more vulnerable to damage by fungi pests. An effective eco-friendly method urgently needed improve their physical chemical properties, decay resistance anti-mildew hydrophobic properties. Here, was heated methyl silicone oil. The effect different temperatures...
Abstract In cereal grains, starch is synthesized by the concerted actions of multiple enzymes on surface granules within amyloplast. However, little known about how starch-synthesizing access granules, especially for amylopectin biosynthesis. Here, we show that rice (Oryza sativa) floury endosperm9 (flo9) mutant defective in biosynthesis, leading to grains exhibiting a endosperm with hollow core. Molecular cloning revealed FLO9 encodes plant-specific protein homologous Arabidopsis...
Summary Endosperm is the main storage organ in cereal grain and determines yield quality. The molecular mechanisms of heat shock proteins regulating starch biosynthesis endosperm development remain obscure. Here, we report a rice floury mutant flo24 that develops abnormal grains central starchy cells. Map‐based cloning complementation test showed FLO24 encodes protein HSP101, which localized plastids. mutated T296I dramatically lost its ability to hydrolyze ATP rescue thermotolerance defects...
Summary Rice ( Oryza sativa L.) accumulates prolamines and glutelins as its major storage proteins. Glutelins are synthesized on rough endoplasmic reticulum 57‐kDa precursors; they then sorted into protein vacuoles where processed acidic basic subunits. We report a novel rice glutelin mutant, W379 , which higher levels of the precursor. Genetic analysis revealed that phenotype is controlled by single recessive nuclear gene. Using map‐based cloning strategy, we identified this gene, OsVPE1...
The rice somaclonal mutant T3612 produces small grains with a floury endosperm, caused by the loose packing of starch granules. positional cloning mutation revealed deletion in gene encoding protein disulphide isomerase-like enzyme (PDIL1-1). In wild type, PDIL1-1 was expressed throughout plant, but most intensely developing grain. T3612, its expression abolished, resulting decrease activity plastidial phosphorylase and pullulanase, an increase that soluble synthase I ADP-glucose...
Chloroplasts and mitochondria contain their own genomes transcriptional translational systems. Establishing these genetic systems is essential for plant growth development. Here we characterized a mutant form of Val-tRNA synthetase (OsValRS2) from Oryza sativa that targeted to both chloroplasts mitochondria. A single base change in OsValRS2 caused virescent albino phenotypes seedlings white panicles at heading. We therefore named this panicle 1 (wp1). Chlorophyll autofluorescence...
Coat protein complex II (COPII) mediates the first step of anterograde transport newly synthesized proteins from endoplasmic reticulum (ER) to other endomembrane compartments in eukaryotes. A group evolutionarily conserved (Sar1, Sec23, Sec24, Sec13, and Sec31) constitutes basic COPII coat machinery; however, details how assembly is regulated remain unclear. Here, we report a mutant rice (Oryza sativa), named glutelin precursor accumulation4 (gpa4), which accumulates 57-kD precursors forms...
Seed-setting rate is a critical determinant of grain yield in rice (Oryza sativa L.). Rapid and healthy pollen tube growth the style required for high seed-setting rate. The molecular mechanisms governing this process remain largely unknown. In study, we isolate dominant low mutant, sss1-D. Cellular examination results show that blocked about half mutant styles. Molecular cloning functional assays reveals SSS1-D encodes OsCNGC13, member cyclic nucleotide-gated channel family. OsCNGC13...
Summary Endosperm, the major storage organ in cereal grains, determines grain yield and quality. Despite fact that a role for P‐type pentatricopeptide repeat ( PPR ) proteins regulation of endosperm development has emerged, molecular functions many remain obscure. Here, we report rice defective mutant, floury endosperm10 flo10 ), which developed smaller starch grains starchy abnormal cells aleurone layer. Map‐based cloning rescued experiments showed FLO 10 encodes protein with 26 motifs, is...
Rice is vulnerable to cold stress. Seedlings are very sensitive stress and this harms global rice production. The effects of on chloroplast development well known, but little known about the underlying molecular mechanisms. Here, we isolated a temperature-sensitive virescent (tsv) mutant that extremely It displayed defective chloroplasts, decreased chlorophyll zero survivorship under We identified TSV by map-based cloning rescue experiments, combined with genetic, cytological biological...
Starch is the most important form of energy storage in cereal crops. Many key enzymes involved starch biosynthesis have been identified. However, molecular mechanisms underlying regulation are largely unknown. In this study, we isolated a novel floury endosperm rice (Oryza sativa) mutant flo16 with defective grain (SG) formation. The amylose content and amylopectin structure were both altered mutant. Map-based cloning complementation tests demonstrated that FLO16 encodes NAD-dependent...
Rice (Oryza sativa) is a facultative short-day (SD) plant, flowering early under SD and late long-day (LD) conditions. Ghd7 major regulator of time in rice, which strongly delays LD. Induction expression by phytochromes has been shown to contribute photoperiodic regulation rice. Here, we show that also regulated at post-transcriptional level. We found constitutive the wild-type (WT) background, but not se5 mutant background (deficient functional phytochromes) LD protein fails accumulate...