A5050118501- MicroRNA in disease regulation
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Ferroptosis and cancer prognosis
- interferon and immune responses
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Lymphoma Diagnosis and Treatment
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Macrophage Migration Inhibitory Factor
- Immunotherapy and Immune Responses
- Cancer-related gene regulation
- Phagocytosis and Immune Regulation
- Peptidase Inhibition and Analysis
- Lung Cancer Treatments and Mutations
- Cancer Genomics and Diagnostics
- Protease and Inhibitor Mechanisms
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Extracellular vesicles in disease
- Viral-associated cancers and disorders
Sun Yat-sen University
2019-2025
Sun Yat-sen Memorial Hospital
2019-2025
University of Hong Kong
2021-2022
Zhongshan Hospital of Xiamen University
2015-2021
Xiamen University
2011-2018
MicroRNAs are stable and easy to detect in plasma. The plasma levels of microRNAs often changed disease conditions, including cancer. This makes circulating a novel class biomarkers for cancer diagnosis. Analyses online microRNA data base revealed that expression level three microRNAs, microRNA-24 (miR-24), microRNA-320a (miR-320a), microRNA-423-5p (miR-423-5p) were down-regulated colorectal (CRC). However, whether the these can serve as CRC diagnosis prognosis is not determined.Plasma...
Abstract Background Circular RNAs (circRNAs), a novel class of noncoding RNAs, have recently drawn much attention in the pathogenesis human cancers. However, role circRNAs esophageal squamous cell carcinoma (ESCC) remains unclear. In this study, we aimed to identify that regulate ESCC progression and explored their regulatory mechanisms clinical significance ESCC. Methods Differentially expressed between paired adjacent normal tissues were identified using microarrays. The effects specific...
Suppression of type I interferon (IFN) response is one pathological outcome the infection highly pathogenic human coronaviruses.To effect this, severe acute respiratory syndrome coronavirus (SARS-CoV) and SARS-CoV-2 encode multiple IFN antagonists.In this study, we reported on antagonism main protease NSP5.NSP5 proteins both SARS-CoV counteracted Sendai virus-induced production.NSP5 variants G15S K90R commonly seen in circulating strains retained IFN-antagonizing property.The suppressive...
SARS-CoV-2 is the causative agent of COVID-19. Overproduction and release proinflammatory cytokines are underlying cause severe Treatment this condition with JAK inhibitors a double-edged sword, which might result in suppression cytokine storm concurrent enhancement viral infection, since signaling essential for host antiviral response. Improving current inhibitor therapy requires detailed molecular analysis on how modulates interferon (IFN)-induced activation JAK-STAT signaling.In study, we...
RAS genes are among the most frequently mutated proto-oncogenes in cancer. However, how Ras stability is regulated remains largely unknown. Here, we report a regulatory loop involving E3 ligase Nedd4-1, Ras, and PTEN. We found that signaling stimulates expression of which turn acts as an ubiquitin regulates levels. Importantly, activation, either by oncogenic mutations or epidermal growth factor (EGF) signaling, prevents Nedd4-1-mediated ubiquitination. This leads to Ras-induced Nedd4-1...
Abstract Mitochondrial dynamics can regulate Major Histocompatibility Complex (MHC)-I antigen expression by cancer cells and their immunogenicity in mice patients with malignancies. A crucial role the mitochondrial fragmentation connection is played IRE1α-XBP-1s axis. XBP-1s a transcription factor for aminopeptidase TPP2, which inhibits MHC-I complex cell surface likely degrading tumor peptides. fission inhibition Mdivi-1 upregulates on enhances efficacy of adoptive T therapy patient-derived...
Abstract Background Esophageal squamous cell carcinoma (ESCC) is a common gastrointestinal tumor and has become an important global health problem. The PI3K/AKT signaling pathway plays key role in the development of ESCC. CircRNAs have been reported to be involved regulation pathway, but underlying mechanisms are unclear. Therefore, this study aimed identify protein-coding circRNAs investigate their functions Methods Differential expression between ESCC tissues adjacent normal was identified...
Abstract Basal‐like breast cancer (BLBC) is the most aggressive molecular subtype of with worse prognosis and fewer treatment options. The underlying mechanisms upon BLBC transcriptional dysregulation its upstream transcription factors (TFs) remain unclear. Here, among hyperactive candidate TFs identified by bioinformatic analysis, POU4F1 uniquely upregulated in associated poor prognosis. necessary for tumor growth malignant phenotypes through regulating G1/S transition direct binding at...
Cyclin-dependent kinase subunit 2 (CKS2) is indicated in the processes of cell cycle and proliferation. Through these processes, CKS2 identified as a cancer gene, but its role has not been well reviewed. The aim present study was to summarize clinicopathological significance molecular mechanisms human cancers. Its expression upregulated majority types studied. shown have function cancers digestive tract, genital thyroid, nerve certain other cancer. can promote progression via positive...
Apoptosis has long been recognized as a significant mechanism for inhibiting tumor formation, and plethora of stimuli can induce apoptosis during the progression treatment tumors. Moreover, tumor-derived apoptotic extracellular vesicles (apoEVs) are inevitably phagocytosed by live cells, promoting heterogeneity. Understanding which apoEVs regulate cells is imperative enhancing our knowledge metastasis recurrence. Herein, we conducted series in vivo vitro experiments, report that promoted...
Smurf1-mediated RhoA ubiquitination and degradation plays key roles in regulation of cell polarity protrusive activity. However, how Smurf1 recognizes is still not clear. Here we report that the C2 domain necessary sufficient for binding RhoA, therefore crucial targeting ubiquitination. In contrast, dispensable Smad1. Consistent with its biochemical specificity, essential Smurf1-regulated protrusion formation but BMP signaling. Therefore, our study reveals mechanism substrate selection.
Abstract miRNAs are expected to become potential biomarkers in the diagnosis and prognosis of Esophageal cancer (EC). Through a series screening, miR-34a-5p, miR-148a-3p miR-181a-5p were selected as EC-associated miRNAs. Based on AllGlo probe, novel absolute quantitative RT-qPCR method with high sensitivity, specificity accuracy was established for detecting Then clinical significance these 3 explored 213 patients (166 cases EC 47 benign diseases) 170 normal controls. Compared controls,...
Abstract Primary pulmonary lymphoepithelioma-like carcinoma (PPLELC) is a rare subtype of non-small-cell lung cancer. Duo to the current lack precise targeted therapies, there an urgent need identify novel therapeutic targets. In this study, we perform single-nucleus transcriptome analysis on PPLELC samples reveal molecular tumor heterogeneity and characterize functional states immune cells within microenvironment. We critical malignant subpopulation characterized by elevated expression AKT3...
Background: In microenvironment of malignant tumors, Hypoxia-Inducible Factors (HIF), most importantly HIF-1α, play an important role in regulation adaptive biological response to hypoxia, promoting angiogenesis and metastasis. However, the underlying mechanism that HIF-1α regulates metastasis needs be further clarified. Methods: The expressions SP1 were detected 182 samples esophageal squamous cell carcinoma (ESCC) adjacent normal tissues by immunohistochemistry (IHC), correlation between...
Abstract Esophageal squamous carcinoma (ESCC) is a common malignant cancer. Although the non-coding roles of circRNAs in pathogenesis human tumors have been well studied, whether participate progression ESCC by encoding novel proteins remains unclear. In this study, we identified an overexpression circRNA with protein-coding ability tissues, called circUBE4B, whose expression level correlated tumor size and differentiation patients. Moreover, higher circUBE4B patients worse prognosis....
Abstract Purpose Solid tumors such as lung adenocarcinoma include not only the tumor cells but also microenvironment in which continuously interact with each other. An in-depth understanding of oncological features and (TME) brain metastases at single-cell level could provide new therapeutic strategies for from adenocarcinoma. Methods To solve this problem, we performed RNA sequencing (scRNA-seq) analysis on 15 samples 10 metastasis samples. Results A total 86,282 single were obtained...
Accumulating evidence indicates that a wide range of E3 ubiquitin ligases are involved in the development many human diseases. Searching for small-molecule modulators these is emerging as promising drug discovery strategy. Here, we report cell-based high-throughput screening method to identify by integrating ubiquitin-reference technique (URT), based on fusion protein located between interest and reference moiety, with Dual-Luciferase system. Using this method, screened SMAD regulatory...
Background. Lipid metabolism reprogramming played an important role in cancer occurrence, development, and immune regulation. The aim of this study was to identify validate lipid metabolism-related genes (LMRGs) associated with the phenotype, prognosis, immunological characteristics lung squamous cell carcinoma (LUSC). Methods. In TCGA cohort, bioinformatics survival analysis were used differentially expressed (DEGs) prognosis LUSC. PTGIS/HRASLS knockdown overexpression effects on LUSC...
Abstract Our previous work demonstrates that DHX32 is upregulated in colorectal cancer (CRC) compared to its adjacent normal tissues. However, how overexpressed contributes CRC remains largely unknown. In this study, we reported was human colon cells. Overexpressed promoted SW480 cells proliferation, migration and invasion, as well decreased the susceptibility chemotherapy agent 5-Fluorouracil. Furthermore, PCR array analyses revealed depleting suppressed expression of WISP1 , MMP7 VEGFA Wnt...
We previously reported that overexpression of DHX32 contributes to the growth and metastasis colorectal cancer (CRC). However, underlying mechanism is not largely characterized. Herein, we in CRC cells upregulated expression vascular endothelial factor A (VEGFA) at transcription level through interacting with stabilizing β-catenin. This promoted recruitment host tumor, therefore, formation microvessel tumor. Xenograft model revealed depletion significantly reduced density grafts suppressed...
The present study explored the oncogenic roles of overexpressed Cks1 and Cks2 in human hepatocellular carcinoma cells. Gene expression HepG2 cells was disrupted by siRNA or increased cDNA transfection. Cell proliferation assayed CCK-8 analysis cell counting. Cisplatin-induced apoptosis after transfection measured flow cytometry using Annexin V/propidium iodide (PI) double staining. cycle changes were determined with PI Protein levels Akt GSK-3β results revealed that decreased depletion...
Aim: To investigate whether plasma miR-19a can serve as a biomarker for esophageal squamous cell carcinoma (ESCC) diagnosis and prognosis. Materials & methods: Plasma samples from 89 ESCC, 45 benign lesion patients 80 healthy controls were subjected to RT-qPCR analyses miR-19a. In addition, 30 collected before after surgery the same analyses. Results: was significantly increased in ESCC compared with controls. The sensitivity of early stages 68.09%. Combination cytokeratin 19 fragment 21–1...