A5044246879- Telomeres, Telomerase, and Senescence
- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Virus-based gene therapy research
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Genomics and Chromatin Dynamics
- Cancer Cells and Metastasis
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Vision and Imaging
- Animal Genetics and Reproduction
- Cancer Research and Treatments
- Science, Research, and Medicine
- Mesenchymal stem cell research
- Biomedical and Engineering Education
- Ubiquitin and proteasome pathways
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer-related Molecular Pathways
- Industrial Vision Systems and Defect Detection
- DNA Repair Mechanisms
- Renal function and acid-base balance
- Epigenetics and DNA Methylation
- Medical Imaging and Pathology Studies
Pennsylvania State University
2003-2025
Washington State University Spokane
2014-2025
XinHua Hospital
2023-2025
Shanghai Jiao Tong University
2022-2025
Shaanxi University of Technology
2023
Washington State University
2015-2020
Block Center
2015
Penn State Milton S. Hershey Medical Center
2014-2015
Hershey (United States)
2010
American Cancer Society
2009
Circulating tumor cells (CTCs) have become an important biomarker for early cancer diagnosis, prognosis, and treatment monitoring. Recently, a replication-competent recombinant adenovirus driven by human telomerase gene (hTERT) promoter was shown to detect live CTCs in blood samples of patients. Here, we report new class adenoviruses containing regulatory elements that repress the hTERT normal cells. Compared virus with only core promoter, viruses showed better selectivity replication than...
The oncogenes RAS and RAF came to view as agents of neoplastic transformation. However, in normal cells, these genes can have effects that run counter oncogenic transformation, such arrest the cell division cycle, induction differentiation, apoptosis. Recent work has demonstrated elicits proliferative senescence mouse human fibroblasts. Because Raf/MEK/MAP kinase signaling cascade is a key effector from Ras proteins, we examined ability conditionally active forms Raf-1 elicit cycle cells....
Human fibroblasts whose lifespan in culture has been extended by expression of a viral oncogene eventually undergo growth crisis marked failure to proliferate. It proposed that telomere shortening these cells is the property limits their proliferation. Here we report ectopic wild-type reverse transcriptase protein (hTERT) human telomerase averts crisis, at same time reducing frequency dicentric and abnormal chromosomes. Surprisingly, as resulting immortalized containing active continue...
The analysis of circulating tumour cells (CTCs) in cancer patients could provide important information for therapeutic management. Enrichment viable CTCs permit performance functional analyses on to broaden understanding metastatic disease. However, this has not been widely accomplished. Addressing challenge, we present a separable bilayer (SB) microfilter size-based CTC capture. Unlike other single-layer microfilters, the precise gap between two layers and architecture pore alignment result...
Overexpression of the proto-oncogene MYC has been implicated in genesis diverse human cancers. One explanation for role tumorigenesis that this gene might drive cells inappropriately through division cycle, leading to relentless proliferation characteristic neoplastic phenotype. Herein, we report overexpression alone cannot sustain cycle normal but instead leads their arrest G 2 . We used an inducible form protein stimulate and rodent fibroblasts. The stimulated passed 1 S arrested...
Human telomerase gene hTERT is important for cancer and aging. promoter regulated by multiple transcription factors (TFs) its activity dependent on the chromatin environment. However, it remains unsolved how interplay between TFs environment controls transcription. In this study, we employed recombinase-mediated BAC targeting recombineering techniques to dissect functions of two proximal E-box sites at −165 +44 nt in regulating native genomic contexts. Our data showed that mutations these...
Pluripotent stem cells (PSCs) express telomerase and have unlimited proliferative potential. To study activation during reprogramming, 3 classes of embryonic cell (ESC)-like clones were isolated from mouse fibroblasts containing a transgenic hTERT reporter. Class I expressed few pluripotency markers, whereas class II contained many, but not Oct4, Nanog, Sox2. Neither differentiated efficiently. III cells, the fully reprogrammed induced PSCs (iPSCs), all formed teratomas indistinguishable...
Background: Long non-coding RNAs (lncRNAs) are crucial factors affecting the occurrence, progression, and prognosis of gastric carcinoma (GC). The accumulation disulfide bonds to excessive levels in cells expressing high SLC7A11 triggers disulfidptosis, which functions as a regulated form cellular death. Research has demonstrated that upregulated is common human cancers, but effect disulfidptosis on GC remains unclear. Identifying lncRNAs associated with (drlncRNAs) establishing prognostic...
The transcriptional activation of human telomerase reverse transcriptase (hTERT) is an important step during cellular immortalization and tumorigenesis. To study how this occurs immortalization, we have established a set genetically related pre-crisis cells their immortal progeny. As expected, hTERT mRNA was detected in our telomerase-positive but not or telomerase-negative cells. However, transiently transfected luciferase reporters controlled by promoter sequences exhibited similar levels...
Purpose: Both nonthyroidal illness syndrome (NTIS) and disseminated intravascular coagulation (DIC) are commonly occurred in sepsis. The objective of this study is to evaluate the association between NTIS DIC, as well their impacts on mortality adults with Patients methods: A total 1219 septic patients two Chinese academic centers from October 2012 2022 were enrolled analysis. We conduct logistic regression models analyze independent risk factors for DIC. Modified Poisson used estimate...
The ANLN gene encodes anillin, a protein that binds to actin. Recent research has identified ANLN’s function in the initiation and advancement of different cancers. However, its impact on gallbladder cancer (GBC) remains unexplored. This study aimed elucidate possible molecular mechanisms GBC. expression was assessed using quantitative real-time polymerase chain reaction (QRT-PCR), Western blotting (WB), immunohistochemistry (IHC), revealing elevated levels GBC tissues. knockdown resulted...
Normal human cells rarely undergo spontaneous immortalization. Given that ectopic expression of the telomerase catalytic subunit hTERT leads to cellular immortalization, endogenous gene is likely constitutively repressed. Hence, we have examined chromatin structure native locus and neighboring loci, CRR9 Xtrp2, in normal fibroblasts a set immortal lines. Using generalized DNase I sensitivity assays, revealed entire was embedded domain as resistant nuclease well studied β-globin loci both...
Abstract Telomerase expression, resulting from transcriptional activation of the hTERT gene, allows cells to acquire indefinite proliferative potential during cellular immortalization and tumorigenesis. However, mechanisms gene in many immortal cell lines cancer are poorly understood. Here, we report our studies on using genetically related pairs telomerase‐negative (Tel − ) ‐positive + fibroblast lines. First, whereas transiently transfected plasmid reporters did not recapitulate endogenous...
The human telomerase reverse transcriptase hTERT is highly expressed in undifferentiated embryonic cells and silenced the majority of somatic cells. To investigate mechanisms silencing, we have developed a novel reporter using bacterial artificial chromosome (BAC) that contained entire gene its neighboring loci, hCRR9 hXtrp2. Firefly Renilla luciferases were used to monitor transcription from promoters, respectively. In mouse stem stably integrated with BAC reporter, both promoters...
Differential regulation of telomerase reverse transcriptase ( TERT ) contributes to the distinct aging and tumorigenic processes in humans mice. Here, we report that hTERT gene was strongly repressed during differentiation human cells, whereas modest mTERT expression detected terminally differentiated post-mitotic cells. The stringent repression depended on native chromatin environment because transiently transfected promoters were not Conversely, core promoter cell differentiation,...
Telomerase is tightly regulated in humans relative to mice, owing the differential regulation of TERT genes. To explore hTERT vivo,we engineered mice with a 160-kb transgenic bacterial artificial chromosome (BAC) spanning locus ReniUa luciferase (Rluc) cassette downstream its promoter. Analysis multiple founder lines revealed that Rluc expression profile from reporter reproduced native gene all tissues and organs examined, demonstrating genetic sequence determined species-specific...