A5100748340- Telomeres, Telomerase, and Senescence
- Platelet Disorders and Treatments
- Immune Cell Function and Interaction
- Diabetes Treatment and Management
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Advanced biosensing and bioanalysis techniques
- Chromosomal and Genetic Variations
- Cancer-related molecular mechanisms research
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- T-cell and B-cell Immunology
- Bacterial Genetics and Biotechnology
- Blood properties and coagulation
- Pluripotent Stem Cells Research
- Hematopoietic Stem Cell Transplantation
- Animal Genetics and Reproduction
- Circular RNAs in diseases
- T-cell and Retrovirus Studies
- Global Health Care Issues
- RNA and protein synthesis mechanisms
- Chronic Lymphocytic Leukemia Research
- Pancreatic function and diabetes
- Esophageal Cancer Research and Treatment
- Immune cells in cancer
Qilu Hospital of Shandong University
2018-2025
Shandong University
2020-2025
Washington State University Spokane
2016-2025
Hainan General Hospital
2022-2024
China Three Gorges University
2023-2024
Shanghai Normal University
2023
Wuhan University
2017-2023
Zhejiang University
2023
Inner Mongolia University
2018-2022
Hainan Medical University
2022
Altered microenvironmental stiffness is a hallmark of inflammation. It sensed by the mechanically activated cation channel Piezo1 in macrophages to induce subsequent immune responses. However, mechanism which mechanosensitive signals shape metabolic status and tune responses remains unclear. We revealed that Piezo1-deficient exhibit reduced aerobic glycolysis resting or liposaccharide (LPS)-stimulated with impaired LPS-induced secretion inflammatory cytokines vitro. Additionally,...
Abstract Background Vasculopathy is the most common complication of diabetes. Endothelial cells located in innermost layer blood vessels are constantly affected by flow or vascular components; thus, their mechanosensitivity plays an important role mediating regulation. damage, one main causes hyperglycemic complications, has been extensively studied. However, mechanosensitive signaling endothelial damage remains unclear. Methods Vascular endothelial-specific Piezo1 knockout mice were...
Soil microorganisms play a key role in soil P transformation and cycling grassland ecosystems, while the microbial mechanisms of animal grazing mediating processes are not fully understood. We conducted experiment typical steppe Inner Mongolia, to explore responses communities rhizosphere bulk soils intensity for understanding affecting cycling. determined physico-chemical properties alkaline phosphatase activities, measured diversity relative abundance P-transformation genes by metagenomics...
Natural products are critical for drug discovery and development; however their is challenged by the wide inactivation or silence of microbial biosynthetic pathways. Currently strategies targeting this problem mainly concentrated on chromosome dissembling, transcription, translation-stage regulations as well chemical stimulation. In study, we developed a novel approach to awake cryptic/silenced pathways through augmentation conserved protein modification step-phosphopantetheinylation carrier...
Cancer immunotherapy by immune checkpoint blockade has been effective in the treatment of certain tumors. However, association between checkpoints and autoimmune diseases remains elusive requires urgent investigation. Primary thrombocytopenia (ITP), characterized reduced platelet count a consequent increased risk bleeding, is an disorder with hyper-activated T cell response. Here, we investigated contribution checkpoint-related single-nucleotide polymorphisms (SNPs), including CD28, ICOS,...
ABSTRACT In Drosophila, an adult external sensory organ (bristle) consists of four distinct cells which arise from a precursor cell via two rounds asymmetric divisions. The first divides to generate secondary cells, IIa and IIb. then produce the hair socket cell. Shortly after, IIb neuron sheath membrane-associated protein Numb has been shown be required for We now report that new hypo- morphic numb mutant not only displays double-socket phenotype, due transformation, but also double-sheath...
Two phorbol ester-inducible elements (beta E2 and beta E3) within the human T-cell receptor gene enhancer each contain consensus binding sites for Ets core factor (CBF) transcription families. Recombinant Ets-1 purified CBF bound individually to E3, in which are directly adjacent. In this report, we show that bind together E3 Ets-1-CBF-DNA complexes favored over of either protein alone E2. Formation increased affinity Ets-1-DNA interactions decreased rate dissociation from DNA. were not...
During asymmetric cell division, the membrane-associated Numb protein localizes to a crescent in mitotic progenitor and is segregated predominantly one of two daughter cells. We have identified putative serine/threonine kinase, Numb-associated kinase (Nak), which interacts physically with phosphotyrosine binding (PTB) domain Numb. The PTB domains Shc insulin receptor substrate bind an NPXY motif not present region Nak that domain. found but through peptide 11 amino acids, implicating novel...
The Moloney murine leukemia virus causes thymic leukemias when injected into newborn mice. A major genetic determinant of the disease specificity genetically maps to two protein binding sites in enhancer, factor b site and adjacent core site. Point mutations introduced either these significantly shifts from erythroleukemia (N. A. Speck, B. Renjifo, E. Golemis, T. Frederickson, J. Hartley, N. Hopkins, Genes Dev. 4:233-242, 1990). We have purified several polypeptides that bind enhancer. These...
Pluripotent stem cells (PSCs) express telomerase and have unlimited proliferative potential. To study activation during reprogramming, 3 classes of embryonic cell (ESC)-like clones were isolated from mouse fibroblasts containing a transgenic hTERT reporter. Class I expressed few pluripotency markers, whereas class II contained many, but not Oct4, Nanog, Sox2. Neither differentiated efficiently. III cells, the fully reprogrammed induced PSCs (iPSCs), all formed teratomas indistinguishable...
Abstract Immune thrombocytopenia (ITP) is an autoimmune disorder, in which megakaryocyte dysfunction caused by reaction can lead to thrombocytopenia, although the underlying mechanisms remain unclear. Here, we performed single-cell transcriptome profiling of bone marrow CD34 + hematopoietic stem and progenitor cells (HSPCs) determine defects megakaryopoiesis ITP. Gene expression, cell-cell interactions, transcriptional regulatory networks varied HSPCs ITP, particularly immune cell...
Abstract Recent scientific studies have highlighted the importance of long-chain noncoding RNAs (lncRNAs) in a variety metabolic diseases, but specific functions and mechanisms lncRNAs aberrant lipid synthesis associated with aging are unknown. In this work, we inspected effects on metabolism mice, as substantial evidence suggests that disturbs metabolism. The results revealed expression lncRNA Gm15232 was significantly elevated epididymal white adipose tissue mice compared to adult mice....
The transcriptional activation of human telomerase reverse transcriptase (hTERT) is an important step during cellular immortalization and tumorigenesis. To study how this occurs immortalization, we have established a set genetically related pre-crisis cells their immortal progeny. As expected, hTERT mRNA was detected in our telomerase-positive but not or telomerase-negative cells. However, transiently transfected luciferase reporters controlled by promoter sequences exhibited similar levels...
Abstract Background: Macrophage polarization anomalies and dysfunction play a crucial role in the pathogenesis of immune thrombocytopenia (ITP). Itaconate is Krebs cycle-derived immunometabolite synthesized by myeloid cells to modulate cellular metabolism inflammatory responses. This study aimed evaluate immunoregulatory effects an itaconate derivative on macrophages patients with ITP. Methods: Peripheral blood-derived from ITP healthy controls were treated 4-octyl (4-OI), that can penetrate...
Abstract Telomerase expression, resulting from transcriptional activation of the hTERT gene, allows cells to acquire indefinite proliferative potential during cellular immortalization and tumorigenesis. However, mechanisms gene in many immortal cell lines cancer are poorly understood. Here, we report our studies on using genetically related pairs telomerase‐negative (Tel − ) ‐positive + fibroblast lines. First, whereas transiently transfected plasmid reporters did not recapitulate endogenous...
The uvrB gene belongs to the SOS network, encoding a key component of nucleotide excision repair. promoter region contains three identified promoters with four LexA binding sites, one consensus and six potential DnaA sites. More than 3-fold increase in transcription chromosomal is observed both ∆lexA ∆sulA cells dnaAA345S cells, 5-fold relative wild-type shown. full activity requires uvrBp1-2 uvrBp3 repressed by proteins. binds tightly LexA-box1 at irrespective presence this important for...
Summary Immune thrombocytopenia (ITP) is an acquired autoimmune disease, in which the imbalance of CD4 + T cell subsets play a key role pathogenesis. Since cells highly depend on metabolism for their function, we hypothesized that dysfunction may be due to intracellular metabolic reprogramming. We found ITP, shifts from oxidative phosphorylation glycolysis. Empagliflozin, sodium–glucose cotransporter 2 inhibitor, has shown regulatory effects proximal tubular epithelial and cardiac beyond...
The human telomerase reverse transcriptase hTERT is highly expressed in undifferentiated embryonic cells and silenced the majority of somatic cells. To investigate mechanisms silencing, we have developed a novel reporter using bacterial artificial chromosome (BAC) that contained entire gene its neighboring loci, hCRR9 hXtrp2. Firefly Renilla luciferases were used to monitor transcription from promoters, respectively. In mouse stem stably integrated with BAC reporter, both promoters...