A5036508844- Single-cell and spatial transcriptomics
- Immune cells in cancer
- Zebrafish Biomedical Research Applications
- High Altitude and Hypoxia
- Immune Cell Function and Interaction
- Effects of Radiation Exposure
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Mesenchymal stem cell research
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Erythrocyte Function and Pathophysiology
- CAR-T cell therapy research
- Cardiac Arrest and Resuscitation
- Osteoarthritis Treatment and Mechanisms
- Digital Media Forensic Detection
- Thermal Regulation in Medicine
- IL-33, ST2, and ILC Pathways
- Gut microbiota and health
- Scoliosis diagnosis and treatment
- Facial Rejuvenation and Surgery Techniques
- Immune responses and vaccinations
- Ideological and Political Education
Beijing Radiation Center
2024-2025
Beijing Tsinghua Chang Gung Hospital
2025
Tsinghua University
2025
Ministry of Ecology and Environment
2025
Tianjin First Center Hospital
2024
Tianjin Medical University
2024
Chinese PLA General Hospital
2019-2023
Academy of Military Medical Sciences
2019-2022
Second Hospital of Shanxi Medical University
2020
Shanxi Medical University
2020
Tracing the emergence of first hematopoietic stem cells (HSCs) in human embryos, particularly scarce and transient precursors thereof, is so far challenging, largely due to technical limitations material rarity. Here, using single-cell RNA sequencing, we constructed genome-scale gene expression landscape covering entire course endothelial-to-HSC transition during embryogenesis. The transcriptomically defined HSC-primed hemogenic endothelial (HECs) were captured at Carnegie stage (CS) 12-14...
A comprehensive understanding of the cellular heterogeneity and molecular mechanisms underlying development, homeostasis, disease human intervertebral disks (IVDs) remains challenging. Here, transcriptomic landscape 108 IVD cells was mapped using single-cell RNA sequencing three main compartments from young adult healthy IVDs, including nucleus pulposus (NP), annulus fibrosus, cartilage endplate (CEP). The chondrocyte subclusters were classified based on their potential regulatory,...
Human skeletal stem cells (SSCs) have been discovered in fetal and adult long bones. However, the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive. Here we map transcriptional landscape limb buds bones at single-cell resolution to address this fundamental question. We found remarkable heterogeneity within bud mesenchyme epithelium, aligned them along proximal-distal anterior-posterior axes using known marker genes. Osteo-chondrogenic progenitors...
Abstract Bone marrow serves as the residence of hematopoietic stem cells and is recognized one most radiosensitive tissues. Exposure to acute radiation leads severe damage bone hematopoiesis which can be fatal, while few clinically applicable medication or specific therapeutic targets have been discovered. In this study, we found that administration cannabidiol significantly enhanced individual survival restored reconstitution capacity within 14 days after irradiation. Single‐cell RNA...
Abstract Whereas the critical roles of innate lymphoid cells (ILCs) in adult are increasingly appreciated, their developmental hierarchy early human fetus remains largely elusive. In this study, we sorted hematopoietic stem/progenitor cells, progenitors, putative ILC progenitor/precursors and mature ILCs fetal hematopoietic, non-lymphoid tissues, from 8 to 12 post-conception weeks, for single-cell RNA-sequencing, followed by computational analysis functional validation at bulk levels. We...
Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells (PSCs) is a central aim in cell regenerative medicine. To date, preferentially reconstituting vivo PSCs remains practical challenge. Here we documented that synergistic transient expression Runx1 Hoxa9 restricted the time window endothelial-to-hematopoietic transition hematopoietic maturation stages PSC differentiation scheme (iR9-PSC) vitro induced preferential generation engraftable progenitors...
Abstract Keratinocyte proliferation and differentiation in epidermis are well-controlled essential for reacting to stimuli such as ultraviolet light. Imbalance between is a characteristic feature of major human skin diseases psoriasis squamous cell carcinoma. However, the effect keratinocyte metabolism on remains largely elusive. We show here that gluconeogenic enzyme fructose-1,6-bisphosphatase 1 (FBP1) promotes while inhibits suppresses development. FBP1 identified among most upregulated...
Aims/Background Patients receiving kidney transplant experience immunosuppression, which increases the risk of bacterial, viral, fungal, and parasitic infections. Q fever is a potentially fatal infectious disease that affects immunocompromised renal recipients has implications in terms severe consequences for donor's kidney. Case Presentation A patient with acute infection following transplantation was admitted to Tsinghua Changgung Hospital Beijing, China, March 2021. Next-generation...
High altitude pulmonary edema (HAPE) poses a significant medical challenge to individuals ascending rapidly high altitudes. Hypoxia-induced cellular morphological changes in the alveolar-capillary barrier such as mitochondrial structural alterations and cytoskeletal reorganization, play crucial role pathogenesis of HAPE. These are critical understanding response hypoxia represent potential therapeutic targets. However, there is still lack effective valid drug discovery strategies for...
ABSTRACT Grain supply and demand affect regional food security; however, the drivers are often unclear, making precise forecasting policymaking challenging. This study used Central Asia as a case to integrate Partial Least Squares Structural Equation Modeling (PLS‐SEM) with particle swarm optimization least squares support vector machine (PSO‐LSSVM) separately identify of grain enhance prediction accuracy. We analyzed interannual variations in production, import/export volumes, consumption,...
Hepatotoxicity induced by bioactive constituents in traditional Chinese medicines or herbs, such as bavachin (BV) Fructus Psoraleae, has a prolonged latency to overt drug-induced liver injury the clinic. Several studies have described BV-induced damage and underlying toxicity mechanisms, but little attention been paid deciphering of organisms cellular responses BV at no-observed-adverse-effect level, molecular mechanisms specific indicators are also lacking during asymptomatic phase, making...
In the aorta-gonad-mesonephros (AGM) region of mouse embryos, pre-hematopoietic stem cells (pre-HSCs) are generated from rare and specialized hemogenic endothelial (HECs) via endothelial-to-hematopoietic transition, followed by maturation into bona fide hematopoietic (HSCs). As HECs also generate a lot progenitors not fated to HSCs, powerful tools that pre-HSC/HSC-specific become urgently critical. Here, using gene knockin strategy, we firstly developed an Hlf-tdTomato reporter model...