A5022431342- Single-cell and spatial transcriptomics
- Zebrafish Biomedical Research Applications
- Muscle Physiology and Disorders
- Immune cells in cancer
- Immune Cell Function and Interaction
- Spine and Intervertebral Disc Pathology
- Mesenchymal stem cell research
- Neuroinflammation and Neurodegeneration Mechanisms
- Muscle metabolism and nutrition
- Cancer-related molecular mechanisms research
- Analytical chemistry methods development
- Bioactive Natural Diterpenoids Research
- MicroRNA in disease regulation
- Nutrition and Health in Aging
- Musculoskeletal pain and rehabilitation
- Viral Infectious Diseases and Gene Expression in Insects
- Erythrocyte Function and Pathophysiology
- Phytochemistry and biological activity of medicinal plants
- Adipose Tissue and Metabolism
- Natural product bioactivities and synthesis
- Advancements in Battery Materials
- RNA modifications and cancer
- Advanced Battery Materials and Technologies
- Phytochemistry and Biological Activities
- Circular RNAs in diseases
Inner Mongolia Electric Power Survey & Design Institute (China)
2025
Chinese Academy of Agricultural Sciences
2021-2024
Lanzhou Institute of Husbandry and Pharmaceutical Sciences
2021-2024
Utrecht University
2024
Chinese PLA General Hospital
2023
Daping Hospital
2023
Army Medical University
2023
Academy of Military Medical Sciences
2019-2022
Gansu Agricultural University
2022
Zhejiang University
2022
Tracing the emergence of first hematopoietic stem cells (HSCs) in human embryos, particularly scarce and transient precursors thereof, is so far challenging, largely due to technical limitations material rarity. Here, using single-cell RNA sequencing, we constructed genome-scale gene expression landscape covering entire course endothelial-to-HSC transition during embryogenesis. The transcriptomically defined HSC-primed hemogenic endothelial (HECs) were captured at Carnegie stage (CS) 12-14...
A comprehensive understanding of the cellular heterogeneity and molecular mechanisms underlying development, homeostasis, disease human intervertebral disks (IVDs) remains challenging. Here, transcriptomic landscape 108 IVD cells was mapped using single-cell RNA sequencing three main compartments from young adult healthy IVDs, including nucleus pulposus (NP), annulus fibrosus, cartilage endplate (CEP). The chondrocyte subclusters were classified based on their potential regulatory,...
Aberrant glycosylation is a pathological alteration that widespread in colon cancer, and usually accompanies the onset progression of disease. To date, molecular mechanisms underlying aberrant remain largely unknown. In this study, we identify somatic germ-line mutations gene encoding for polypeptide N-acetylgalactosaminyltransferase 12 (GALNT12) individuals with cancer. Biochemical analyses demonstrate each 8 GALNT12 identified inactivates normal function GALNT enzyme initiating mucin type...
Human skeletal stem cells (SSCs) have been discovered in fetal and adult long bones. However, the spatiotemporal ontogeny of human embryonic SSCs during early skeletogenesis remains elusive. Here we map transcriptional landscape limb buds bones at single-cell resolution to address this fundamental question. We found remarkable heterogeneity within bud mesenchyme epithelium, aligned them along proximal-distal anterior-posterior axes using known marker genes. Osteo-chondrogenic progenitors...
Abstract Whereas the critical roles of innate lymphoid cells (ILCs) in adult are increasingly appreciated, their developmental hierarchy early human fetus remains largely elusive. In this study, we sorted hematopoietic stem/progenitor cells, progenitors, putative ILC progenitor/precursors and mature ILCs fetal hematopoietic, non-lymphoid tissues, from 8 to 12 post-conception weeks, for single-cell RNA-sequencing, followed by computational analysis functional validation at bulk levels. We...
Exposure to microgravity leads alterations in multiple systems, but microgravity-related changes the gastrointestinal tract and its clinical significance have not been well studied. We used hindlimb unloading (HU) mouse model simulate a condition investigated intestinal microbiota colonic epithelial cells. Compared with ground-based controls (Ctrls), HU affected fecal composition profile that was characterized by expansion of Firmicutes decrease Bacteroidetes. The colon epithelium mice...
Background: The discovery of traditional plants’ medicinal and nutritional properties has opened up new avenues for developing pharmaceutical dietary strategies to prevent atherosclerosis. However, the effect antioxidant Dendrobium officinale polysaccharide (DOP) on atherosclerosis is still not elucidated. Purpose: This study aims investigate inhibitory potential mechanism DOP high-fat diet-induced in Apolipoprotein E knockout (ApoE−/−) mice. Study design methods: identification was measured...
Abstract Disuse leads to severe muscle atrophy and a slow-to-fast myofiber-type transition. PGC-1α (Peroxisome proliferator-activated receptor γ coactivator 1α) is documented play an important role in slow-twitch myofiber determination. Transcription of atrophy-related Atrogin-1 by FoxO3 can be reduced PGC-1α. While Smad3 augments FoxO3-induced MuRF1 promoter activity. So PGC-1α, as transcription co-activator, may regulate hindlimb unloading (HU)-induced transition through Smad3. Our results...
Rhubarb has edible stems or stalks. In this paper, we investigated the nutritional value, chemical composition, and bioactivities of Rheum palmatum (SRP) analyzed mode action. SRP exhibited biosafety had with abundant essential amino acids minerals. Based on network pharmacology western blot tests, found that it showed anti-inflammatory activity via PI3K-Akt-mediated NF-κB pathway. Out 20 compounds identified using UPLC-ESI-Q-TOF/MS analysis, cirsiliol hydrangenol were active they inhibited...
Muscle biopsy has long been expected to be replaced by noninvasive biomarkers with diagnostic value and prognostic applications for muscle atrophy. Growing evidence suggests that circulating microRNAs (miRNAs) could act as numerous pathophysiological statuses. In the present study, our results showed serum levels of six muscle-specific miRNAs (miR-1/23a/133/206/208b/499) were all elevated in unloading induced mice. The medium these starvation atrophic C2C12 myotubes. Moreover,...
Abstract Background Muscle wasting occurs in response to various physiological and pathological conditions, including ageing Duchenne muscular dystrophy (DMD). Transforming growth factor‐β1 (TGF‐β1) contributes muscle pathogenesis elderly people DMD patients; inhibition of TGF‐β1 signalling is a promising therapeutic strategy for muscle‐wasting disorders. Hemojuvelin (HJV or Hjv as the murine homologue) membrane‐bound protein that highly expressed skeletal muscle, heart, liver. In hepatic...
Abstract Previous studies have revealed cellular heterogeneity in intervertebral discs (IVDs). However, the and molecular alteration patterns of cell populations during degenerative progression remain to be fully elucidated. To illustrate disc degeneration (IDD), we perform single RNA sequencing on cells from four anatomic sites healthy goat IVDs. EGLN3 + StressCs, TGFBR3 HomCs GPRC5A RegCs exhibit characteristics associated with resistance stress, maintaining homeostasis repairing,...
Backgroud: Rhododendron przewalskii Maxim. is an evergreen shrub that used as a traditional medicine in China. However, the modern pharmacology and chemical components of this plant has not been studied. In paper, we aimed to investigate antifungal, anti-inflammatory antioxidant activities underlying mechanism its aqueous ethanol extracts, analyze their composition active compounds R. . Methods: The antifungal activity was determined vitro , extracts were evaluated RAW 264.7 cells. analyzed...
Background: Sarcopenia is an age-related condition characterized by progressive loss of muscle mass, strength, and function. The occurrence sarcopenia has a huge impact on physical, psychological, social health. Therefore, the prevention treatment becoming important public health issue. Method: 35 six-week-old male C57BL/6 mice were randomly divided into five groups, one which served as control group, while rest groups constructed model intraperitoneal injection D-galactose. intervention...
The aim of this study is to investigate the effects inhibiting Aurora-B on osteosarcoma (OS) cell malignant phenotype, phosphorylation valosin-containing protein (VCP), and activity NF-κB signaling in vitro. expressions p-VCP proteins were detected by immunohistochemistry 24 OS tissues, relationship between was investigated. results showed that there a positive correlation proteins. expression human lines U2-OS HOS cells inhibited specific short hairpin RNA (shRNA) lentivirus (AURKB-shRNA...