A5013518261- Advanced Proteomics Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Extracellular vesicles in disease
- Prostate Cancer Treatment and Research
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Cancer Genomics and Diagnostics
- Molecular Biology Techniques and Applications
- Ferroptosis and cancer prognosis
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Lung Cancer Research Studies
- RNA Research and Splicing
- Lung Cancer Treatments and Mutations
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer, Lipids, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Mass Spectrometry Techniques and Applications
- Aortic Disease and Treatment Approaches
- Computational Drug Discovery Methods
- Pancreatic and Hepatic Oncology Research
- Genetic and Kidney Cyst Diseases
- Antimicrobial agents and applications
- Aortic aneurysm repair treatments
Asan Medical Center
2009-2025
University of Ulsan
2002-2024
Korea Institute of Science and Technology
2014-2023
Ulsan College
2002-2022
Convergence
2021
Korea University of Science and Technology
2014-2018
Bio-Medical Science (South Korea)
2018
Korea Advanced Institute of Science and Technology
2009-2010
Saga University
2003
Korea Electrotechnology Research Institute
1999
Abstract Background Glioblastoma (GBM) is more difficult to treat than other intractable adult tumors. The main reason that GBM so it highly infiltrative. Migrasomes are newly discovered membrane structures observed in migrating cells. Thus, they can be generated from cells have the ability migrate along brain parenchyma. However, function of migrasomes has not yet been elucidated Results Here, we describe composition and with cell migration. Proteomic analysis revealed LC3B-positive...
Eukaryotic translation initiation factor 2α (eIF2α) phosphorylation, which regulates all three unfolded protein response pathways, helps maintain cellular homeostasis and overcome endoplasmic reticulum (ER) stress through transcriptional translational reprogramming. However, regulation of mitochondrial by eIF2α phosphorylation during ER is not fully understood. Here, we report that the phosphorylation-activating transcription 4 (ATF4) axis required for expression multiple factors (TFs)...
The effects of sunitinib in a broad patient population, especially those Asian ethnicity, have been rarely investigated. Here, we assessed the efficacy and safety Korean patients with advanced renal cell carcinoma.Between April 2006 August 2008, 77 carcinoma were treated sunitinib. We performed retrospective analysis for terms survival outcomes response rate. Toxicity profiles also assessed.A total 65 patients, including 39 (60%) without previous cytotoxic or immunotherapy, eligible...
We found that non-small-cell lung cancer (NSCLC) cells express high levels of multiple aldehyde dehydrogenase (ALDH) isoforms via an informatics analysis metabolic enzymes in NSCLC and immunohistochemical staining clinical tumor samples. Using a reaction-monitoring mass spectrometry analysis, we ALDH isozymes were generally abundant compared with their normal IMR-90 human cells. As result the catalytic reaction mediated by ALDH, NADH is produced as by-product from conversion to carboxylic...
Brain-derived exosomes released into the blood are considered a liquid biopsy to investigate pathophysiological state, reflecting aberrant heterogeneous pathways of pathological progression brain in neurological diseases. provide promising prospects for diagnosis diseases, with exciting possibilities early and sensitive such However, capability traditional exosome isolation assays specifically isolate characterize brain-derived exosomal proteins by high-throughput proteomics clinical...
Lymphocyte-rich hepatocellular carcinoma (LR-HCC) is largely unknown and a rare subtype of HCC with immune-rich stroma. Tertiary lymphoid structures (TLS), frequently observed in LR-HCC, are known to be prognostically significant various malignancies; however, their significance remains unevaluated.
Renal dysfunction, a major complication of type 2 diabetes, can be predicted from estimated glomerular filtration rate (eGFR) and protein markers such as albumin concentration. Urinary biomarkers may used to monitor or predict patient status. Urine samples were selected patients enrolled in the retrospective diabetic kidney disease (DKD) study, including 35 with good 19 poor prognosis. After removal immunoglobulin, remaining proteins reduced, alkylated, digested, analyzed qualitatively...
The 5-year survival rate in the early and late stages of ovarian cancer differs by 63%. In addition, a liquid biopsy is necessary because there are no symptoms stage tissue collection difficult without using invasive methods. Therefore, need for biomarkers to achieve this goal. study, we found blood-based metabolite or protein biomarker candidates diagnosis 20 clinical samples (10 patients 10 healthy control subjects). Plasma metabolites proteins were measured quantified mass spectrometry...
Non-small cell lung cancer (NSCLC) is histologically classified into adenocarcinoma (LUAD) and squamous carcinoma (LSCC). However, some tumors are ambiguous other pathophysiological features or microenvironmental factors may be more prominent. Here we report integrative multiomics analyses using data for 229 patients from a Korean NSCLC cohort 462 previous studies. Histological examination reveals five molecular subtypes, one of which subtype with PI3K-Akt pathway upregulation, showing high...
We propose to use cRFP (common Repository of FBS Proteins) in the MS (mass spectrometry) raw data search cell secretomes. is a small supplementary sequence list highly abundant fetal bovine serum proteins added reference database use. The aim behind using prevent contaminant from being misidentified as other database, just we would cRAP Adventitious present either by accident or through unavoidable contacts proteins. expect it be widely used experiments where are obtained serum-free media...
Extracellular vesicles (EVs) are emerging as crucial biomarkers in cancer diagnostics and therapeutics with their heterogeneity presenting both challenges opportunities prostate research. However, existing methods for isolating characterizing EV subtypes have been limited by inefficient separation inadequate proteomic analysis. Here we show an optimized centrifugal microfluidic device, Exodisc, that efficiently isolates large quantities of from particle-enriched medium, enabling...
The preoperative diagnosis of follicular thyroid carcinoma (FTC) is challenging because it cannot be readily distinguished from adenoma (FA) or benign nodular disease (FND) using the sonographic and cytological features typically employed in clinical practice. We comprehensive proteomics machine learning (ML) models to identify novel diagnostic biomarkers capable classifying three subtypes: FTC, FA, FND. Bottom-up techniques were applied quantify proteins formalin-fixed, paraffin-embedded...
We report a case of malignant pheochromocytoma recurred after debulking surgery. A 17-year-old male patient visited our hospital for right flank pain. He had not experienced palpitations, headache, sweating or weight loss. Level urinary catecholamine and its metabolite increased above normal values abdominal computed tomography showed huge adrenal mass. One month surgery, anterior mediastinal multiple liver metastases were found. These tumors no response to two conventional regimens...
Objective To determine the change of expression Bcl2 in cisplatin‐resistant bladder cancer cell lines and reversibility chemoresistance to cisplatin with antisense oligonucleotide against Bcl2, as higher is associated drug resistance many different lines. Materials methods In tumour T24R1 T24R2, was determined by reverse transcription‐polymerase chain reaction Western blot assay, targeting coding sequence administered lipofectin. Results The mRNA protein greater T24R2 cells than parent T24...
Aims: The naive or primitive states of stem cells (SCs) residing in specific niches are unstable and difficult to preserve vitro. Vitamin C (VitC), addition suppressing oxygen radicals, exerts pleiotropic effects the core functions SCs. However, this compound is labile readily oxidized, resulting cellular toxicity preventing its reliable application context. We found that a VitC derivative, ascorbic acid 2-glucoside (AA2G), stably maintains pluripotency murine embryonic SCs (mESCs)...
Major depressive disorder (MDD) is a leading cause of global disability with chronic and recurrent course. Recognition biological markers that could predict monitor response to drug treatment personalize clinical decision-making, minimize unnecessary exposure, achieve better outcomes. Four longitudinal plasma samples were collected from each ten patients MDD treated antidepressants for 10 weeks. Plasma proteins analyzed qualitatively quantitatively nanoflow LC-MS/MS technique. Of 1153...
The major challenges in pancreatic ductal adenocarcinoma (PDAC) management are local or distant metastasis and limited targeted therapeutics to prevent it. To identify a druggable target tumor secretome explore its therapeutic intervention, we performed liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic analysis of tumors obtained from patient-derived xenograft model PDAC. Galectin-3 binding protein (Gal-3BP) is identified as highly secreted protein, overexpression...
Non-small-cell lung cancer (NSCLC) constitutes approximately 80% of all diagnosed cancers, and diagnostic markers detectable in the plasma/serum NSCLC patients are greatly needed. In this study, we established a pipeline for discovery using 9 transcriptome datasets from publicly available databases profiling six cell secretomes. Thirty-one out 312 proteins that overlapped between two-fold differentially expressed genes identified secretome were detected pooled plasma patients. To quantify...
Eight aminoacyl-tRNA synthetases (M, K, Q, D, R, I, EP and LARS) three auxiliary proteins (AIMP1, 2 3) are known to form a multi-tRNA synthetase complex (MSC) in mammalian cells. We combined size exclusion chromatography (SEC) with reversed-phase liquid multiple reaction monitoring mass spectrometry (RPLC-MRM-MS) characterize MSC components free ARS human embryonic kidney (HEK 293T) Crude cell extract affinity-purified were fractionated by SEC non-denaturing state ARSs monitored each...
A superposition type ozonizer has been proposed to achieve relatively high ozone concentration and generation efficiency. high-pulsed voltage a 60 Hz ac are simultaneously applied the same reaction volume in order improve yield wide discharge gap. The of superimposed consisting surface pulsed corona was approximately two times larger than that obtained by an individual discharge. It observed cross-section extended is be 20% at maximum. emission intensity shows about maximum due increased...
Abstract Variations in protein coding sequence may sometimes play important roles cancer development. However, since variants not express into proteins due to various cellular quality control systems, it is get protein-level evidence of the genomic variations. We present a proteogenomic strategy getting variants, which we call sequential targeted LC-MS/MS based on prediction peptide pI and Retention time (STaLPIR). Our approach shows improved identification, has potential for unbiased...
The identification of blood biomarkers to diagnose acute exacerbation chronic obstructive pulmonary disease (AECOPD) will have clinical utility. Here, we used a proteomics-based approach identify capable identifying AECOPD.This prospective, single-center pilot study enrolled 12 patients who came Asan Medical Center (South Korea) via the outpatient clinic or emergency department with symptoms AECOPD and were follow-up in during convalescence between 2015 2017. Paired samples collected from...
Rationale: Exposure to humidifier disinfectants (HDs) can increase the risk of asthma, but characteristics HD-related asthma are currently unclear. Polyhexamethylene guanidine (PHMG)–containing HD was most commonly used and frequently associated with HD-associated lung injury. Objectives: To investigate PHMG-induced asthma. Methods: This general population–based birth cohort study data from Panel Study Korean Children 2008 (n = 846). Spirometry, bronchial provocation tests, detailed history...