A5100621214- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Circular RNAs in diseases
- Epigenetics and DNA Methylation
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Pharmacological Effects of Natural Compounds
- Medicinal Plant Pharmacodynamics Research
- Genetics and Neurodevelopmental Disorders
- Cancer-related gene regulation
- Ginseng Biological Effects and Applications
- Peptidase Inhibition and Analysis
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Aldose Reductase and Taurine
- Natural product bioactivities and synthesis
- Phytochemistry and Bioactive Compounds
- Cancer, Lipids, and Metabolism
- Cancer Treatment and Pharmacology
- Neuropeptides and Animal Physiology
- Acute Myeloid Leukemia Research
- PI3K/AKT/mTOR signaling in cancer
- Medical Research and Treatments
Hebei Medical University
2025
Second Affiliated Hospital of Zhejiang University
2025
Wenzhou Medical University
2020-2025
First Affiliated Hospital of Kunming Medical University
2019-2024
Kunming Medical University
2019-2024
Nanjing Medical University
2024
Jiangsu Province Hospital
2024
Beijing Union University
2016-2024
Soochow University
2024
Yan'an University
2013-2023
Epidermal growth factor receptor (EGFR) is a clinical therapeutic target to treat subset of non-small cell lung cancer (NSCLC) harboring EGFR mutants. However, some patients with similar kind mutation show intrinsic resistance tyrosine kinase inhibitors (TKI). It indicates that other key molecules are involved in the survival these cells. We showed here 2-[(aminocarbonyl)amino]-5 -(4-fluorophenyl)-3- thiophenecarboxamide (TPCA-1), previously reported inhibitor IκB kinases (IKK), blocked...
Purpose: This study was done to investigate the inhibition effects of miR-30a-3p on mitotic arrest deficient 2 like 1 (MAD2L1) expression and proliferation gastric cancer cells. Patients methods: Cluster analysis TCGA database were used screen key genes highly expressed in cancer. Based LinkedOmics website, correlation between cell cycle-related target gene MAD2L1 analyzed. The mRNA protein levels detected with quantitative real-time PCR Western blot analysis. cycle also Results:...
Abstract Background The role of TMED3 involved in cancers has been seldom described, let alone breast cancer. To explore the clinicopathological significance expression and biological roles cancer cells, we undertook study. Methods Immunohistochemistry was performed to observe pattern tissues, totaling 224 cases; followed by detailed statistical analysis between versus information available. malignant behaviors wound-healing Transwell assays were conducted evaluate variation migration...
Methyl-CpG-binding protein 2 (MeCP2) facilitates the carcinogenesis and progression of several types cancer. However, its role in breast cancer relevant molecular mechanism remain largely unclear. In this study, analysis Cancer Genome Atlas (TCGA) data that MeCP2 expression was significantly upregulated tissues, high correlated with poor overall survival. Knockdown inhibited cell proliferation G1-S cycle transition migration as well induced apoptosis vitro. Moreover, knockdown suppressed...
Abstract Introduction Clear cell renal carcinoma (ccRCC) is the most prevalent and aggressive subtype of carcinoma, originating from tubular epithelial cells in kidney. Hypoxia proves to be a feature commonly observed solid tumors, leading increased resistance treatment tumor progression. Methods scRNA‐seq data were procured GSE159115 set. We utilized UMAP NMF algorithm for clustering dimensionality reduction. The FindAllMarkers function was used compare various groups identify potential...
Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a tetramethylfolate enzyme involved in folate metabolism. The aim of this study to determine the effect MTHFD2 on proliferation and metastasis colorectal cancer (CRC).MTHFD2 was silenced or overexpressed CRC cells. qRT-PCR Western blotting were used analyze mRNA protein expression, respectively. MTT assay colony forming detect cell formation ability. cycle apoptosis changes detected by flow cytometry. Transwell experiments migration...
Chimeric antigen receptor (CAR) T cells targeting CD19 have produced remarkable clinical outcomes for cancer patients. However, identifying measures to enhance effector function remains one of the most challenging issues in CD19-targeted immunotherapy. Here, we report a novel approach which microRNA (miRNA) or short-hairpin RNA (shRNA) cassette was integrated into CAR-expressing retroviral vectors. Using this system, generated anti-CD19 CAR-T co-expressing miR155 LSD1 shRNA and found that...
Abstract EGR1 regulates the expression of its downstream target genes and may exert different biological effects in tumours. We found that was increased gastric cancer (GC), silencing promoted apoptosis GC cells. Moreover, overexpression repressed Bioinformatics analysis showed had binding sites at upstream promoter region miR‐195; ChIP assays were applied to determine occupancy miR‐195 promoter. The RT‐PCR results suppressed miR‐195. mechanism by which acts as a transcriptional repressor is...
miR-497-5p can inhibit cervical cancer cell proliferation. However, the underlying mechanism remains to be elucidated.Bioinformatics was used analyze target genes of miR-497-5p. qRT-PCR and Western blot were mRNA protein expression, respectively. Dual-luciferase reporter assay direct binding between 3'-untranslated region CBX4. Cell viability measured with MTT assay. Flow cytometry performed detect cycle distribution.Here, using bioinformatics methods we firstly found that regulated...
Castrate-resistant prostate cancer (CRPC) is an aggressive and lethal disease. The pathogenesis of CRPC not fully understood novel therapeutic targets need to be identified improve the patients' prognosis. MicroRNA-30a (miR-30a) has been demonstrated a tumor suppressor in many types solid malignancies. However, its role androgen-independent (AI) growth (PCa) received limited attention as yet.The clinical association miR-30a potential with AI was characterized by bioinformatics analyses....
Abstract Background Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression miR-335-5p has been reported many cancers, including gastric (GC). In this study, precise GC as well molecular mechanisms underlying its effects, role target MAPK10, were evaluated. Methods Quantitative real-time PCR was used to evaluate levels cell lines and tissues. MTT colony formation assays detect proliferation, Transwell wound-healing invasion migration...
Apoptosis of endothelial cells (ECs) plays a key role in the development atherosclerosis and there are also evidence indicated that phosphatase tensin homolog deleted on chromosome 10 (PTEN) is viable target therapeutic approaches to prevent vascular ECs apoptosis. Aberrant miR-106b-5p expression has been reported plasma patients with unstable atherosclerotic plaques. However, underlying mechanism miR-106-5p genesis have not addressed. In this study, we explored anti-apoptotic by regulating...
Ovarian cancer (OC) is a severe malignancy featuring poor prognosis due to rapid metastasis and chemotherapy resistance. In this study, we extensively investigated the upstream downstream mechanisms of miR-548e in regulating OC progression cisplatin Our results indicated that ZFAS1 was highly expressed promoted cell proliferation, migration, invasion, resistance by directly suppressing expression. co-localized with cytosols cells. repressed CXCR4 expression, elevated expression Cisplatin...
Non-small cell lung cancer (NSCLC) accounts for approximately 80-85% of all cancers, with the FAS-associated factor 1 (FAF1) acting as a tumor suppressor. MicroRNAs (miRNAs) can influence progression by targeting oncogenes or anti-oncogenes. In this study, we aimed to reveal miR-26a-5p on regulation FAF1 expression and NSCLC progression, motivation identifying potential therapeutic target treatment.A dual-luciferase reporter assay was used check direct miR-26a-3p. The inhibitor shRNA plasmid...
Abstract The present study evaluated the anticancer potential of celastrol through down‐regulation matrix metalloproteinase‐2 (MMP‐2) and MMP‐9. HeLa cells were incubated with different concentrations (1, 10 100 µM) for 48h. Doxorubicin was used as a reference drug. Cancer cell migration, apoptosis, viability mitochondrial fragmentation following treatment. In addition, expression level MMP‐2, MMP‐9 caspase‐3 94.1 ± 7, 53.4 4 36.3 2% at 1‐100 µM celastrol, respectively. Apoptotic numbers...
Introduction Taurine has a prominent lipid-lowering effect on hyperlipidemia. However, comprehensive analysis of the effects taurine endogenous metabolites in hyperlipidemia not been documented. This study aimed to explore impact multiple associated with Methods The hyperlipidemic mouse model was induced by high-fat diet (HFD). administered via oral gavage at doses 700 mg/kg/day for 14 weeks. Evaluation body weight, serum lipid levels, and histopathology liver adipose tissue performed...