A5027643455- Melanoma and MAPK Pathways
- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Virus-based gene therapy research
- Chemotherapy-induced cardiotoxicity and mitigation
- Computational Drug Discovery Methods
- Cutaneous Melanoma Detection and Management
- Nonmelanoma Skin Cancer Studies
- PARP inhibition in cancer therapy
- Ubiquitin and proteasome pathways
- Cancer-related Molecular Pathways
- Cancer, Stress, Anesthesia, and Immune Response
- Hippo pathway signaling and YAP/TAZ
- vaccines and immunoinformatics approaches
- Neuroscience and Neuropharmacology Research
- Wnt/β-catenin signaling in development and cancer
- Skin and Cellular Biology Research
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Neuroscience and Neural Engineering
- Peptidase Inhibition and Analysis
- Protein Degradation and Inhibitors
- Cardiac electrophysiology and arrhythmias
- Hepatitis B Virus Studies
- Dermatologic Treatments and Research
George Washington University
2016-2024
GW Medical Faculty Associates
2017-2020
Washington University in St. Louis
2008-2015
Saint Louis University
2003-2014
Baoshan College
2010
Inserm
1996
Institut pour la Recherche sur le Cancer de Lille
1996
The efficiency of hepatitis C virus (HCV) transmission by sexual activity remains controversial. We conducted a cross-sectional study HCV-positive subjects and their partners to estimate the risk for HCV infection among monogamous heterosexual couples. A total 500 anti-HCV-positive, human immunodeficiency virus-negative index long-term were studied. Couples interviewed separately lifetime factors infection, within-couple practices, sharing personal grooming items. Blood samples tested...
Acute mu and kappa opioids activate the ERK/MAPK phosphorylation cascade that represents an integral part of signaling pathway growth factors in astrocytes. By this cross-talk, may impact neural development plasticity among other basic neurobiological processes vivo. The agonist, [D-ala2,mephe4,glyol5]enkephalin (DAMGO), induces a transient stimulation ERK phosphorylation, whereas U69,593, engenders sustained activation. Here we demonstrate acute U69,593 DAMGO stimulate by utilization...
GTP binding regulatory protein (G protein)-coupled receptors can activate MAPK pathways via G protein-dependent and -independent mechanisms. However, the physiological outcomes correlated with cellular signaling events are not as well characterized. In this study, we examine involvement of beta-arrestin 2 in kappa opioid receptor-induced, extracellular signal-regulated kinase 1/2 (ERK1/2)-mediated proliferation both immortalized primary astrocyte cultures. As different agonists induce...
Growth factors, hormones, and neurotransmitters have been implicated in the regulation of stem cell fate. Since various neural precursors express functional neurotransmitter receptors, which include G protein-coupled it is anticipated that they are involved fate decisions. We detected mu-opioid receptor (MOR-1) kappa-opioid (KOR-1) expression immunoreactivity embryonic (ES) cells retinoic acid-induced ES cell-derived, nestin-positive, progenitors. Moreover, these receptors functional, since...
Seven‐ transmembrane receptors activate ERK/MAPK via G protein dependent and independent mechnanisms. Opioid (ORs) modulate proliferation of immortalized primary astrocytes ERK. We examined κ OR‐induced, ERK‐mediated rat to delineate participation vs. β‐arrestin (β‐arr). opioid agonist, U69,593, activated ERK by a rapid (min) initial stimulation that was sustained over 2 h increased as measured bromodeoxyuridine labeling. MOM‐Sal‐B (a salvinorin derivative), induced activation transiently...
Proline-rich protein tyrosine kinase 2 (Pyk2) is a member of the focal adhesion family. We used Pyk2 knockout (Pyk2-KO) mice to study role in cutaneous wound repair. report that rate closure was delayed Pyk2-KO compared with control mice. To examine whether impaired healing caused by keratinocyte cell-autonomous defect, capacities primary keratinocytes from and wild-type (WT) littermates heal scratch wounds vitro were compared. The repair decreased WT cells. Moreover, cultured human...
The efficacy of an anthracycline antibiotic doxorubicin (DOX) as a chemotherapeutic agent is limited by dose-dependent cardiotoxicity. DOX associated with activation intracellular stress signaling pathways including p38 MAPKs. While previous studies have implicated MAPK in DOX-induced cardiac injury, the roles individual isoforms, specifically, alternative isoforms p38γ and p38δ, remain uncharacterized. We aimed to determine potential cardioprotective effects p38δ genetic deletion mice...
One of the several still unexplained aspects mechanism by which Cdc34/SCF RING-type ubiquitin ligases work is marked stimulation Cdc34 autoubiquitination, a phenomenon unknown and significance. In in vitro experiments with single-lysine-containing mutant proteins Saccharomyces cerevisiae, we found that SCF-mediated autoubiquitination limited to specific N-terminal lysines modified via an intermolecular mechanism. striking contrast, SCF quenches C-terminal catalyzed intramolecular manner....
In this work, we show several previously unknown features of p120-catenin in a cadherin-catenin complex that are critical for our understanding cadherin-based adhesion and signaling. We human epithelial A-431 cells, nearly all p120 molecules engage high-affinity interaction with E-cadherin-catenin complexes located at the cellular surface. is positioned proximity to alpha-catenin cadherin. These findings suggest functional cooperation between adhesion. A low level cadherin-free molecules,...
Activation and/or upregulated expression of p38δ are demonstrated in human skin malignancies including cutaneous squamous cell carcinoma, suggesting a role for carcinogenesis. We previously reported that mice with germline deletion the gene significantly protected from chemical Here, we investigated effects cell-selective targeted ablation keratinocytes and immune (myeloid) cells on tumor development two-stage 7,12-dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA)...
e12569 Background: Triple-Negative Breast Cancers (TNBCs) represent a diverse subset of tumors characterized by varied responses to therapies including immunotherapy (IO). While IO is approved in early-stage and advanced TNBC, are modest. Modulation the tumor microenvironment improve an active area research. In-vitro in-vivo studies have shown Poly-ADP Ribose Polymerase inhibitors (PARPi) increase PD-L1 expression sporadic, BRCA1 BRCA2 mutated TNBC cell lines improving response IO. In...
To the Editor: Seborrheic keratosis (SK) is one of most common types dermatologic lesions and is, therefore, skin tumor seen by dermatologists in everyday practice.1Coyne J.D. Classification seborrheic keratosis.Int J Surg Pathol. 2016; 24: 51-52Crossref PubMed Scopus (4) Google Scholar Currently, cryosurgery commonly employed successful techniques to physically resolve an SK.2Farhangian M.E. Snyder A. Huang K.E. Doerfler L. W.W. Feldman S.R. Cutaneous United States.J Dermatolog Treat. 27:...
p38δ expression and/or activity are increased in human cutaneous malignancies, including invasive squamous cell carcinoma (SCC) and head neck SCC, but the role of carcinogenesis has not been well-defined. We have reported that mice with germline loss exhibited a reduced susceptibility to skin tumor development compared wild-type two-stage 7,12-dimethylbenz(a)anthracene (DMBA)/12-O-tetradecanoylphorbol-13-acetate (TPA) chemical model. Here, we report gene ablation inhibited growth tumors...
Severe cardiotoxic effects limit the efficacy of doxorubicin (DOX) as a chemotherapeutic agent. Activation intracellular stress signaling networks, including p38 mitogen-activated protein kinase (MAPK), has been implicated in DOX-induced cardiotoxicity (DIC). However, roles individual isoforms DIC remain incompletely elucidated. We recently reported that global p38δ deletion protected female but not male mice from DIC, whereas p38γ did significantly modulate it. Here we studied vivo p38α and...
Hepatitis C virus (HCV) is a major cause of hepatitis and hepatocellular carcinoma (HCC) world-wide. Most HCV patients have relatively stable disease, but approximately 25% progressive disease that often terminates in liver failure or HCC. highly variable genetically, with seven genotypes multiple subtypes per genotype. This variation affects HCV's sensitivity to antiviral therapy has been implicated contribute differences disease. We sequenced the complete viral coding capacity for 107...
BackgroundUV-A radiation contributes to photoaging/photocarcinogenesis by generating inflammation and oxidative damage. Current photoprotective strategies are limited the availability/utilization of UV-A filters, highlighting an unmet need. Cannabidiol (CBD), having anti-inflammatory/antioxidant properties via regulation nuclear erythroid 2–related factor, heme oxygenase 1, peroxisome proliferator-activated receptor gamma, could potentially mitigate damage from exposure.Objective/MethodsThis...