A5101544809- Amyloidosis: Diagnosis, Treatment, Outcomes
- Cardiovascular Function and Risk Factors
- Parathyroid Disorders and Treatments
- Cardiovascular Disease and Adiposity
- Cardiac Fibrosis and Remodeling
- Adipose Tissue and Metabolism
- Cardiovascular Effects of Exercise
- Heart Failure Treatment and Management
- Muscle Physiology and Disorders
- Cardiovascular and exercise physiology
- Angiogenesis and VEGF in Cancer
- Cardiac Imaging and Diagnostics
- Hormonal Regulation and Hypertension
- Cardiomyopathy and Myosin Studies
- Atrial Fibrillation Management and Outcomes
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cardiac Arrhythmias and Treatments
- Venous Thromboembolism Diagnosis and Management
- GDF15 and Related Biomarkers
- Cardiovascular Health and Disease Prevention
- Cardiac Valve Diseases and Treatments
- Sarcoidosis and Beryllium Toxicity Research
- Diet and metabolism studies
- Sirtuins and Resveratrol in Medicine
- Renin-Angiotensin System Studies
Kumamoto University
2014-2025
Tokyo Metropolitan University
2022-2025
Osaka Metropolitan University
2022-2025
Kumamoto University Hospital
2017-2025
Asahikawa Medical University
2025
Metropolitan University
2023-2025
Metropolitan University
2023
Osaka City University
1988-2022
Osaka University
2022
Izumi City General Hospital
2011-2016
Although increased external load initially induces cardiac hypertrophy with preserved contractility, sustained overload eventually leads to heart failure through poorly understood mechanisms. Here we describe a conditional transgenic system in mice characterized by the sequential development of adaptive contractility acute phase and dilated cardiomyopathy chronic following induction an activated Akt1 gene heart. Coronary angiogenesis was enhanced during growth but reduced as hearts underwent...
Fibroblast growth factor‐21 (FGF21) functions as a metabolic regulator. The FGF21 transcript is reported to be abundantly expressed in liver, but little known about the regulation of expression other tissues. In this study, we show that levels protein were similar skeletal muscle and liver from fasted mice. upregulated gastrocnemius muscle‐specific Akt1 transgenic Serum concentration was also increased by transgene activation. cultured cells, secretion regulated insulin, Akt transduction...
Although signaling mechanisms inducing cardiac hypertrophy have been extensively studied, little is known about the that reverse hypertrophy. Here, we describe existence of a similar Akt/forkhead axis in myocytes vitro and vivo, which regulated by insulin, insulin-like growth factor (IGF), stretch, pressure overload, angiotensin II stimulation. FOXO3a gene transfer prevented both IGF stretch-induced rat neonatal myocyte cultures vitro. Transduction with also caused significant reduction...
Cardiac hypertrophy is associated with upregulation of vascular endothelial growth factor (VEGF) in the myocardium. Here, we evaluated effects a decoy VEGF receptor on heart morphology and function to murine model pressure overload hypertrophy. Mice were administered adenoviral vector encoding (Ad-Flk), their hearts subjected by transverse aortic constriction (TAC). Treatment Ad-Flk led net reduction capillary density TAC. also TAC-induced cardiac promoted left ventricle dilatation loss...
Myogenic Akt signaling coordinates blood vessel recruitment with normal tissue growth. Here, we investigated the role of Follistatin-like 1 (Fstl1) in regulation endothelial cell function and growth muscle. Transgenic Akt1 overexpression skeletal muscle led to myofiber that was coupled an increase capillary density. or ischemic hind limb surgery induction Fstl1 increased circulating levels Fstl1. Intramuscular administration adenoviral vector expressing (Ad-Fstl1) accelerated flow recovery...
Objective— We investigated the comparative roles of mitogen-activated protein (MAP) kinases, including c-Jun NH2-terminal kinase (JNK), extracellular signal-regulated (ERK), and p38, in vascular smooth muscle cell (VSMC) proliferation, migration, gene expression. Methods Results— VSMCs were infected with recombinant adenovirus containing dominant-negative mutants ERK, JNK (Ad-DN-ERK, Ad-DN-p38, Ad-DN-JNK, respectively) to specifically inhibit respective MAP kinases then stimulated...
The Akt protein kinase is an important mediator of cardiac myocyte growth and survival. To identify factors with novel therapeutic applications in diseases, we focused on the identification secreted from Akt1-activated cells that have cardioprotective effects through autocrine/paracrine mechanisms.Using inducible Akt1 transgenic mouse model, found follistatin-like 1 (Fstl1) transcript expression are increased 4.0- 2.0-fold, respectively, by activation heart (P<0.05). Fstl1 was also...
Multiple lines of evidence establish that angiotensin II (Ang II) induces not only hypertension but also directly contributes to cardiac diseases. Apoptosis signal-regulating kinase 1 (ASK1), one mitogen-activated protein kinases, plays a key role in stress-induced cellular responses. However, nothing is known about the ASK1 hypertrophy and remodeling vivo. In this study, by using mice deficient (ASK1 −/− mice), we investigated induced Ang II. Left ventricular (LV) was activated infusion...
Cannabinoid 1 (CB1) receptor blockade with rimonabant represents a clinical therapeutic strategy for obesity. Recently, the role of endocannabinoid system has been described in peripheral organs. We sought to determine whether could be involved human atherosclerosis and CB1 modulate proinflammatory activity macrophages.mRNA expression levels coronary atherectomy samples were significantly higher patients unstable angina than those stable (3.62+/-2.96-fold; n=7; P<0.05). Immunoreactive area...
The detailed role of angiotensin II in salt-exacerbated stroke is unclear. We examined the salt-accelerated stroke-prone spontaneously hypertensive rats (SHRSP).Salt-loaded SHRSP were orally given type 1 (AT1) receptor blocker candesartan (0.3 to 3 mg/kg per day) and calcium channel amlodipine (1 day), effects on (n=61) brain superoxide compared between them. also effect infusion (200 ng/kg min) production blood-brain barrier.Despite comparable hypotensive amlodipine, prolonged survival...
Although the mammalian heart is one of least regenerative organs in body, recent evidence indicates that myocardium undergoes a certain degree renewal to maintain homeostasis during normal aging. However, cellular origin cardiomyocyte has remained elusive due lack lineage tracing experiments focusing on putative adult cardiac precursor cells. We have generated triple-transgenic mice based tet-cre system identify descendants cells expressed stem cell marker Sca1. found significant and lasting...
Heart failure, which is a major global health problem, often preceded by pathological cardiac hypertrophy. The expansion of the vasculature, to maintain adequate supply oxygen and nutrients, key determinant whether heart grows in physiological compensated manner or decompensated manner. Bidirectional endothelial cell (EC)-cardiomyocyte (CMC) cross talk via cardiokine angiocrine signaling plays an essential role regulation growth homeostasis. Currently, mechanisms involved EC-CMC interaction...
Several studies have reported that colchicine attenuated the infarct size and inflammation in acute myocardial infarction (MI). However, sustained benefit of administration on survival cardiac function after MI is unknown. It was hypothesized short-term treatment with could improve during recovery phase MI.Methods Results:MI induced mice by permanent ligation left anterior descending coronary artery. Mice were then orally administered 0.1 mg/kg/day or vehicle from 1 h to day 7 MI. Colchicine...
Aging is associated with the development of insulin resistance, increased adiposity, and accumulation ectopic lipid deposits in tissues organs. Starting mid-life there a progressive decline lean muscle mass preferential loss glycolytic, fast-twitch myofibers. However, it not known to what extent metabolic dysfunction are causally related or whether they independent epiphenomena aging process. Here, we utilized skeletal-muscle-specific, conditional transgenic mouse expressing constitutively...
Abstract Aim The aim of this study was to evaluate the diagnostic utility high‐sensitivity cardiac troponin T (hs‐cTnT) levels in discriminating amyloidosis from patients with hypertrophy caused by aetiologies other than amyloidosis. Methods and results Serum hs‐cTnT were measured 96 (light chain: 23, wild‐type transthyretin amyloidosis: 40, mutated 33), 91 causes who confirmed have no endomyocardial biopsy (control group). cut‐off value evaluated receiver operating characteristic analysis....
BACKGROUND: MRI-derived myocardial extracellular volume fraction (ECV) is elevated in the presence of fibrosis, amyloid deposition, inflammation and edema. In patients with cardiac amyloidosis prolonged T2 due to concomitant or edema, MRI-ECV may not correctly reflect histological load. The authors sought determine whether can accurately load 2 groups wild-type transthyretin cardiomyopathy (ATTRwt-CM), without prolongation. METHODS: This retrospective study included consecutive ATTRwt-CM who...
Duchenne muscular dystrophy, the most common form of childhood is caused by X-linked inherited mutations in dystrophin gene. Dystrophin deficiencies result loss dystrophin–glycoprotein complex at plasma membrane, which leads to structural instability and muscle degeneration. Previously, we induced muscle-specific overexpression Akt, a regulator cellular metabolism survival, mdx mice pre-necrotic (<3.5 weeks) ages demonstrated upregulation utrophin–glycoprotein protection against...