- Genomics and Phylogenetic Studies
- Pesticide and Herbicide Environmental Studies
- Microbial bioremediation and biosurfactants
- Microbial Community Ecology and Physiology
- Plant-Microbe Interactions and Immunity
- Enzyme-mediated dye degradation
- Microbial Natural Products and Biosynthesis
- Legume Nitrogen Fixing Symbiosis
- Pharmaceutical and Antibiotic Environmental Impacts
- Iron Metabolism and Disorders
- Monoclonal and Polyclonal Antibodies Research
- Aquaculture disease management and microbiota
- Microbial metabolism and enzyme function
- Nematode management and characterization studies
- Oral and gingival health research
- Biofuel production and bioconversion
- Drug-Induced Adverse Reactions
- Wastewater Treatment and Nitrogen Removal
- Analytical chemistry methods development
- Molecular Biology Techniques and Applications
- Plant Disease Resistance and Genetics
- Biosensors and Analytical Detection
- Odor and Emission Control Technologies
- Mesoporous Materials and Catalysis
- Identification and Quantification in Food
Zhoukou Normal University
2019-2025
Nanjing Agricultural University
2014-2017
An indole–3–acetic acid producing Bacillus altitudinis WR10 was previously isolated from the root of wheat (Triticum aestivum L.). In this study, strain used for relieving abiotic stresses in under low phosphorus and high saline hydroponic co-culture models. Significantly, improved seed relative germination rate salinity stress (200/400 mM NaCl) dry weight seedlings (10 μM KH2PO3) when insoluble phosphates are available. To provide insights into its stress-alleviating properties,...
Due to the extensive use of chloroacetanilide herbicides over past 60 years, bacteria have evolved catabolic pathways mineralize these compounds. In upstream pathway, are transformed into two common metabolites 2-methyl-6-ethylaniline (MEA) and 2,6-diethylaniline (DEA) through N-dealkylation amide hydrolysis. The pathway downstream MEA is initiated by hydroxylation aromatic rings, followed its conversion a substrate for ring cleavage after several steps. Most key genes in been identified....
Strain BUT-8 T , a Gram-stain-negative, non-motile and rod-shaped aerobic bacterium, was isolated from the activated sludge of herbicide-manufacturing wastewater treatment facility. Comparative 16S rRNA gene sequence analysis revealed that strain clustered with species genus Lysobacter closely related to ruishenii DSM 22393 (98.3 %) daejeonensis KACC 11406 (98.7 %). The DNA G+C content genomic 70.6 mol%. major respiratory quinone ubiquinone-8, polar lipids were diphosphatidylglycerol,...
Thiobencarb is a thiocarbamate herbicide used in rice paddies worldwide. Microbial degradation plays crucial role the dissipation of thiobencarb environment. However, physiological and genetic mechanisms underlying remain unknown. In this study, novel pathway was proposed Acidovorax sp. strain T1. oxidized cleaved at C-S bond, generating diethylcarbamothioic S-acid 4-chlorobenzaldehyde (4CDA). 4CDA then to 4-chlorobenzoic acid (4CBA) hydrolytically dechlorinated 4-hydroxybenzoic (4HBA). The...
A novel aerobic, Gram-stain-negative, motile bacterium, designated strain BUT-10(T), was isolated from the sludge of a pesticide manufacturing factory in Kunshan, China. Cells were rod-shaped (0.4-0.45×0.9-1.4 µm) and colonies white, circular with entire edges had smooth surface. The grew at 25-37 °C, pH 6.0-8.0 0-0.5 % NaCl. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that BUT-10(T) member genus Phenylobacterium, showed highest similarities to Phenylobacterium...
Genetic engineering of probiotics, like bifidobacteria, may improve their microbial cell factory economy. This work designed a novel shuttle plasmid pBPES, which bears exogenous appA and is stable within Bifidobacterium longum JCM 1217. Cloning three predicted promoters into pBPES proved that all them drive expression in B. Transformation plasmids pBPES-tu pBPES-groEL JCM1217 resulted much more phytase secretion suggests Ptu PgroEL are strong promoters. Further vitro vivo experiments...
Bacillus sp. strain WR11 isolated from the root of wheat (Triticum aestivum L.) possesses abiotic stress alleviating properties and produces several types enzymes. However, its genomic information is lacking. The study described complete genome sequence bacterium. size was 4 202 080 base pairs that consisted 405 genes in total. G+C content circular 43.53% there were 170 coding genes, 114 pseudo 30 ribosome RNAs, 86 tRNAs, 5 ncRNAs, based on Prokaryotic Genome Annotation Pipeline (PGAP). Many...
A Gram-stain-positive, rod-shaped, non-motile, non-spore-forming, aerobic bacterial strain, designated BUT-2 T , was isolated from activated sludge of one herbicide-manufacturing wastewater-treatment facility in Kunshan, Jiangsu province, China, and subjected to polyphasic taxonomic studies. Analysis the 16S rRNA gene sequence indicated that strain shared highest similarity with Chryseomicrobium amylolyticum (98.98 %), followed by imtechense (98.88 less than 96 % similarlity members genera...
Quinoline is a typical nitrogen-heterocyclic compound with high toxicity and carcinogenicity which exists ubiquitously in industrial wastewater. In this study, new quinoline-degrading bacterial strain Rhodococcus sp. JH145 was isolated from oil-contaminated soil. Strain could grow quinoline as the sole carbon source. The optimum growth temperature, pH, salt concentration were 30 °C, 8.0, 1%, respectively. 100 mg/L be completely removed within 28 h. Particularly, showed excellent...
A nonphotosynthetic, Gram-stain-negative, rod-shaped and motile strain, designated Pet-1T, was isolated from oil-contaminated soil collected Daqing oil field in China. Optimal growth occurred at 37 °C, pH 5.5 1 % (w/v) NaCl. Q-10 the sole respiratory quinone. The most abundant fatty acid C18 : 1ɷ7c/C18 1ɷ6c (67.4 %). major polar lipids were phosphatidylglycerol, aminolipid, phosphatidylethanolaine, phosphatidycholine, two unidentified phospholipids. genomic DNA G+C content 69.3 mol%....
A rapid immunochromatographic lateral flow strip test (ILFST) of competitive format for the detection diclofenac (DCF) in medicinal wine samples was developed. The half-inhibitory concentration (IC50) values ILFST DCF and limit (LOD) DCF-spiked were 0.66 0.15 ng/mL, respectively. Recoveries from spiked 84.4% to 85.9% within an assay (intra-assay) 83.7% 88.4% between assays (inter-assay). coefficients variation (CV) intra-assay inter-assay 4.10–7.56% 4.71–8.94%, These results indicated that...