Staphylococcus pseudintermedius is a commensal bacterium frequently isolated from canine skin and recognized as zoonotic agent especially for dog-owners. This study focused on (a) the antibiotic-resistance phenotypes; (b) ability to produce biofilm (slime); (c) dissemination of virulence factors in S. strains. Seventy-three strains were screened against 22 different molecules by means Kirby-Bauer assay. The was investigated using microtiter plate assay (MtP) amplification icaA icaD genes....

Abstract A sensitive (5 to 100 µg) and reproducible (±3.54% SD) method is described for determining monoglycerides, diglycerides, triglycerides, free fatty acids. After these lipid classes are separated from extracts of plasma or tissues by a single thin-layer chromatographic development, they eluted into concentrated H2SO4 at 200°C. The resulting color stable more than six days.

Staphylococcus aureus is considered one of the most widespread bacterial pathogens for both animals and humans, being causative agent various diseases like food poisoning, respiratory tract infections, nosocomial bacteremia, surgical site cardiovascular infections in as well clinical subclinical mastitis, dermatitis, suppurative animals. Thanks to their genetic flexibility, several virulent drug-resistant strains have evolved mainly due horizontal gene transfer insurgence point mutations....

Staphylococcus pseudintermedius is an emergent zoonotic agent associated with multidrug resistance (MDR). This work aimed to describe the antibacterial activity of four essential oils (EOs) and silver nanoparticles (AgNPs) against 15 S. strains isolated from pyoderma. The EOs, namely Rosmarinus officinalis (RO), Juniperus communis (GI), Citrus sinensis (AR), Abies alba (AB), AgNPs were used alone in combination determine Minimum Inhibitory Concentration (MIC) Bactericidal (MBC). All MDR...

Background/Objectives: Pseudomonas aeruginosa causes chronic infections in humans and animals, especially cats dogs. This bacterium's ability to adapt acquire antibiotic resistance traits may complicate exacerbate antibacterial therapy. study aimed evaluate the patterns, virulence factors form biofilm of P. strains isolated from Algerian dogs cats. Methods: Nineteen samples were collected healthy diseased Isolates studied for their patterns (disc diffusion method), formation (Microtiter...

Background/Objectives: Pseudomonas aeruginosa causes chronic infections in humans and animals, especially cats dogs. This bacterium’s ability to adapt acquire antibiotic resistance traits may complicate exacerbate antibacterial therapy. study aimed evaluate the patterns, virulence factors form biofilms of P. strains isolated from Algerian dogs cats. Methods: Nineteen samples were collected healthy diseased Isolates studied for their antibiotic-resistance patterns (disc diffusion method)...

Dairy herds around the world are undergoing several changes. Herd sizes increasing, as both milk yield and quality. The implementation of new technologies in various domains dairy production is leading to an increase amount data available. This, turn, creates a need extract useful information from these improve efficiency. This paper presents findings preliminary study that utilizes machine learning (ML) approach assess accuracy somatic cell count (SCC) neutrophils + lymphocytes Count/mL...

Natural hydroxycinnamic acid amides (HCAAs) and riparins offer significant health benefits. However, their extraction from natural sources is difficult, traditional synthetic methods remain wasteful, raising the need for more efficient alternatives. In this work, a two-step chemo-enzymatic flow method esterification amidation of phenolic acids was developed successfully applied to synthesis riparin derivatives HCAAs. The Fischer optimized using vanillic as model starting material SiliaBond...

Dairy herds around the world are undergoing several changes. Herd sizes increasing, as both milk yield and quality. The implementation of new technologies in various domains dairy production is leading to an increase quantity data available. This, turn, creates a need extract useful information from these improve efficiency. This paper presents findings preliminary study that utilizes machine learning (ML) approach assess accuracy somatic cell count (SCC) neutrophils + lymphocytes count/mL...

Background: Segmental bone defects (SBDs) pose significant clinical challenges, often requiring complex reconstructive procedures. Degradable substitutes loaded with antibiotics have emerged as promising tools for infection control. However, their impact on healing remains uncertain. This study investigates antibiotic-loaded biodegradable scaffolds in infected using an vivo rabbit model. Methods: Thirty New Zealand white rabbits were divided into three groups—antibiotic-loaded GreenBone...

Both synthetic and natural rubber-like elastomers are widely employed in industrial applications (such as tires, seals, protective gloves, damping absorbers) well the food animal husbandry industries. These materials should be regularly checked for contamination associated infectious risk since they frequently come into contact with food, animals, people. Additionally, could act vehicle of microbes and, a result, diseases. This pilot study investigates antibacterial efficacy novel elastomer...

Silver nanoparticles (AgNPs) are promising alternatives to antibiotics. The aims of this study were produce AgNPs using two biological methods and determine their antibacterial activity against Pseudomonas aeruginosa Staphylococcus pseudintermedius. biosynthesized from an infusion Curcuma longa (turmeric) the culture supernatant E. coli. Characterization was achieved by ultraviolet-visible spectroscopy Transmission Electron Microscopy (TEM). properties NPs C. (ClAgNPs) coli (EcAgNPs), alone...

Bacterial populations inhabiting a variety of natural and human-associated niches have the ability to grow in form biofilms. A large part pathological chronic conditions, essentially all bacterial infections associated with implanted medical devices or prosthetics, are caused by microorganisms embedded matrix made polysaccharides, proteins, nucleic acids. Biofilm generally characterized slow onset, mild symptoms, tendency chronicity, refractory response antibiotic therapy. Even though...

A series of carbonate and carbamate derivatives natural phenols, tyrosol hydroxytyrosol, were synthetized through a chemo-enzymatic flow approach. First, the chemoselective synthesis hydroxytyrosol carbonates was performed using immobilized lipase B from Candida antarctica as catalyst in tert-amyl alcohol, non-conventional green solvent. Then, two selected reacted with appropriate amine to obtain desired carbamates. All compounds isolated moderate good yields. The showed increased...

Oral colon delivery systems based on a dual targeting strategy, harnessing time- and microbiota-dependent release mechanisms, were designed in the form of drug-containing core, swellable/biodegradable polysaccharide inner layer gastroresistant outer film. High-methoxyl pectin was employed as functional coating polymer applied by spray-coating or powder-layering. Stratification powder required use low-viscosity hydroxypropyl methylcellulose water solution binder. These coatings exhibited...

The conjugated linoleic acid (CLA) isomers, a group of naturally occurring isomers the essential fatty (FA) acid, have received special attention in animal and human nutrition. Although they long been used as dietary integrators dairy cows, effects CLA on bovine immune cells remain mostly undisclosed. present study aimed to cover this gap investigate vitro inflammatory functions, including chemotaxis, phagocytosis, killing capability, extracellular respiratory burst purified monocytes...

Streptococcus agalactiae is a well-known pathogen in humans and food-producing animals. Therefore, this bacterium paradigmatic example of to be controlled by One Health approach. Indeed, the zoonotic reverse-zoonotic potential bacteria, prevalence Group B Streptococci (GBS) diseases both human animal domains, threatening global situation on GBS antibiotic resistance make these bacteria an important target for control programs. An epidemiological analysis using public database containing...

Milk differential somatic cells count (DSCC), made possible under field conditions by the recent availability of a high-throughput milk analyzer may represent an improvement in mastitis diagnosis. While increasing number studies reports data on DSCC individual cow samples, very few concerns from quarter samples. This paper for first time results retrospective study aiming to assess performance total (SCC), DSCC, and novel calculated marker (PLCC) measured samples as method identify cows at...

Staphylococcus pseudintermedius is the main aetiological agent of canine pyoderma. Whole genome sequencing most comprehensive way obtaining relevant genomic information about micro-organisms.Oxford Nanopore technology enables quality and de novo assembly whole S. pseudintermedius. analysis may help to better understand pathogenesis pyodermas.Twenty-two strains isolated from skin five healthy dogs 33 with pyoderma were analysed. DNA was extracted sequenced using Oxford MinION, a new that...

Mastitis is one of the major diseases dairy cows that affects milk quality and quantity increases potential risk for presence antimicrobial residues (AR) in milk, which could lead to development resistance (AMR) among human pathogens. Even if AR products low many countries, threat not negligible cannot be ignored. These problems may investigated by applying a One Health approach, this prevalence study aimed estimate risks health related production applied herds Lombardy. Three hundred...