A5103031286- Chronic Obstructive Pulmonary Disease (COPD) Research
- Gut microbiota and health
- Pediatric health and respiratory diseases
- Tracheal and airway disorders
- Respiratory and Cough-Related Research
- Respiratory viral infections research
- Respiratory Support and Mechanisms
- Asthma and respiratory diseases
- Enterobacteriaceae and Cronobacter Research
- Delphi Technique in Research
- Mycobacterium research and diagnosis
- Oral microbiology and periodontitis research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Antifungal resistance and susceptibility
- Inhalation and Respiratory Drug Delivery
- Dementia and Cognitive Impairment Research
- Health Systems, Economic Evaluations, Quality of Life
- BIM and Construction Integration
- Spatial Neglect and Hemispheric Dysfunction
- Maritime Navigation and Safety
- Cancer Risks and Factors
- Social Media in Health Education
- Spacecraft Design and Technology
- Innovative Approaches in Technology and Social Development
- Chronic Disease Management Strategies
University of Bergen
2013-2025
Haukeland University Hospital
2013-2024
Copenhagen University Hospital
2020-2023
Rigshospitalet
2020-2023
University of Copenhagen
2007-2020
Clinical trials evaluating the management of acute exacerbations COPD assess heterogeneous outcomes, often omitting those that are clinically relevant or more important to patients. We have developed a core outcome set, consensus-based minimum set outcomes we recommend evaluated in all future clinical on management, improve their quality and comparability. were identified through methodological systematic reviews qualitative interviews with 86 patients from 11 countries globally. The most...
Activation of the blood coagulation system is a common observation in inflammatory diseases. The role COPD underexplored.The study included 413 patients and 49 controls from 3-year Bergen Cohort Study (BCCS). One hundred forty-eight were also examined during AECOPD. plasma markers activation, TAT complex, APC-PCI complex D-dimer, measured at baseline exacerbations by enzyme immunoassays. Differences levels between stable controls, AECOPD examined. associations later mortality negative...
The low bacterial load in samples acquired from the lungs, have made studies on airway microbiome vulnerable to contamination DNA introduced during sampling and laboratory processing. We examined impact of collected lower airways by protected (through a sterile catheter) bronchoscopy explored various silico approaches dealing with post-sequencing. Our analyses included quantitative PCR targeted amplicon sequencing 16S rRNA gene. mean varied sample type for 23 study subjects (oral wash>1st...
Background The fungal part of the pulmonary microbiome (mycobiome) is understudied. We report composition oral and mycobiome in participants with COPD compared to controls a large-scale single-centre bronchoscopy study (MicroCOPD). Methods Oral wash bronchoalveolar lavage (BAL) was collected from 93 100 controls. Fungal DNA extracted before sequencing internal transcribed spacer 1 (ITS1) region ribosomal RNA gene cluster. Taxonomic barplots were generated, we taxonomic composition, Shannon...
Background Exacerbations of chronic obstructive pulmonary disease (COPD) are debilitating events and spur progression. Infectious causes frequent; however, it is unknown to what extent exacerbations caused by larger shifts in the airways' microbiota. The aim current study was analyse changes microbial composition between stable state during exacerbations, corresponding immune response. Methods sample included 36 COPD patients examined at exacerbation from Bergen Cohort studies, one patient...
Abstract Background Studies on the airway microbiome have been performed using a wide range of laboratory protocols for high-throughput sequencing bacterial 16S ribosomal RNA (16S rRNA) gene. We sought to determine impact number polymerase chain reaction (PCR) steps (1- or 2- steps) and choice target marker gene region (V3 V4 V4) presentation upper lower microbiome. Our analyses included lllumina MiSeq following three setups: Setup 1 (2-step PCR; V3 region), 2 3 (1-step region). Samples oral...
Introduction Previous population-based studies, mainly from high-income countries, have shown that a higher forced vital capacity (FVC) is associated with lower risk of developing cardiometabolic diseases. The aim this study was to assess the longitudinal association between spirometry measures and onset diseases across sites in low-income, middle-income countries. Methods population comprised 5916 individuals 15 countries participating Burden Obstructive Lung Disease baseline follow-up...
The aim of this study was to investigate whether the compositionality lower airway microbiota predicts later exacerbation risk in persons with COPD a cohort study.We collected airways samples by bronchoalveolar lavage and protected specimen brushes, oral wash from 122 participants COPD. Bacterial DNA extracted all samples, before we sequenced V3-V4 region 16S RNA gene. frequency moderate severe exacerbations surveyed telephone interviews follow-up visit. Compositional taxonomy α β diversity...
Randomised controlled trials (RCTs) on the management of COPD exacerbations evaluate heterogeneous outcomes, often omitting those that are clinically important and patient relevant. This limits their usability comparability. A core outcome set (COS) is a consensus-based minimum outcomes should be evaluated in all RCTs specific areas health care. We present study protocol COS-AECOPD ERS Task Force, aiming to develop COS for exacerbation management, could remedy these limitations. For...
COPD and coronary heart disease (CHD) frequently co-occur, yet which phenotypes are most prone to CHD is poorly understood. The aim of this study was see whether patients did have a true higher risk for than subjects without COPD, examine range potential factors associated with in controls.347 428 non-COPD controls, were invited computed tomography angiography (CCTA) pulmonary CT. Arterial blood gas, bioelectrical impedance lung function measured, detailed medical history taken. CCTA...
Background The lower airway microbiota in patients with chronic obstructive pulmonary disease (COPD) are likely altered compared the healthy individuals. Information on how is affected by smoking, use of inhaled corticosteroids (ICS) and COPD severity still scarce. Methods In MicroCOPD Study, participant characteristics were obtained through standardised questionnaires clinical measurements at a single centre from 2012 to 2015. Protected bronchoalveolar lavage samples 97 controls paired-end...
Data on discomfort and complications from research bronchoscopy in chronic obstructive pulmonary disease (COPD) asthma is limited. We present occurring within a week after bronchoscopy, investigate personal procedural risk factors.239 subjects with COPD, or without lung underwent bronchoscopies as part of microbiome study the lower airways (the MicroCOPD study). Bronchoscopy was done supine position oral scope insertion option light conscious alfentanil sedation. Sampling consisted protected...
The role of the pulmonary microbiome in sarcoidosis is unknown. objectives this study were following: (1) examine whether fungal and bacterial microbiota differed patients with compared controls; (2) there was an association between levels antimicrobial peptides (AMPs) protected bronchoalveolar lavage (PBAL).Thirty-five 35 healthy controls underwent bronchoscopy sampled oral wash (OW), BAL (PBAL), left sterile brushes (LPSB). ITS1 region V3V4 16S rRNA gene sequenced. Bioinformatic analyses...
The lower airways microbiome and host immune response in chronic pulmonary diseases are incompletely understood. We aimed to investigate possible characteristics key antimicrobial peptides proteins idiopathic fibrosis (IPF) obstructive disease (COPD).
Abstract Objective Little is known concerning the stability of lower airway microbiome. We have compared microbiota identified by repeated bronchoscopy in healthy subjects and patients with ostructive lung diseaseases (OLD). Methods 21 controls 41 OLD completed two bronchoscopies. In addition to negative (NCS) oral wash (OW) samples, we gathered protected bronchoalveolar lavage fractions (PBAL1 PBAL2) specimen brushes (PSB). After DNA extraction, amplified V3V4 region 16S rRNA gene,...
Bronchoscopy is frequently used to sample the lower airways in lung microbiome studies. Despite being a safe procedure, it associated with discomfort that may result reservations regarding participation research bronchoscopy Information on studies limited. We report response rates, reasons for non-response, motivation participation, and predictors of large-scale single-centre study (“MicroCOPD”). Two hundred forty-nine participants underwent at least one addition examined by physician,...
Few studies have examined the stability of pulmonary mycobiome. We report longitudinal changes in oral and mycobiome participants with without COPD a large-scale bronchoscopy study (MicroCOPD).Repeated sampling was performed 30 21 COPD. collected an wash (OW) bronchoalveolar lavage (BAL) sample from each participant at two time points. The internal transcribed spacer 1 region ribosomal RNA gene cluster PCR amplified sequenced on Illumina HiSeq sequencer. Differences taxonomy, alpha...
Spirometry is used to determine what "unusual" lung function compared with "usual" for healthy non-smokers. This study aimed investigate regional variation in the forced vital capacity (FVC) and expiratory volume one second FVC ratio (FEV1/FVC) using cross-sectional data from all 41 sites of multinational Burden Obstructive Lung Disease study. Participants (5,368 men; 9,649 women), aged ≥40 years, had performed spirometry, never smoked reported no respiratory symptoms or diagnoses. To...
Background: The aim of this study was to validate dementia diagnoses in the Danish nationwide hospital registers order determine value these epidemiological research on younger patients. Two hundred patients were randomly selected from 891 aged 65 years or and registered with a diagnosis for first time 2008. patients' medical records reviewed evaluate if they fulfilled ICD-10 and/or DSM-IV criteria clinical specific subtypes. Three independent raters records. Data compared data previous...