A5089311549- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Tea Polyphenols and Effects
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Catalysis and Immobilization
- Livestock and Poultry Management
- Animal Nutrition and Physiology
- SARS-CoV-2 and COVID-19 Research
- NF-κB Signaling Pathways
- Biochemical Analysis and Sensing Techniques
- Immunodeficiency and Autoimmune Disorders
- Protein Kinase Regulation and GTPase Signaling
- Cholesterol and Lipid Metabolism
- Respiratory viral infections research
- PI3K/AKT/mTOR signaling in cancer
- Metabolism and Genetic Disorders
- Phytochemicals and Antioxidant Activities
- Spectroscopy and Chemometric Analyses
- Advanced Chemical Sensor Technologies
- Flavonoids in Medical Research
- Enzyme Structure and Function
- Sexual Differentiation and Disorders
- Dermatology and Skin Diseases
Zhejiang University of Technology
2025
Second Affiliated Hospital of Zhejiang University
2015-2025
Indiana University – Purdue University Indianapolis
2021-2024
Indiana University School of Medicine
2021-2024
Stanford University
2018-2024
Southwest University
2024
Jiangnan University
2020-2024
Indiana University
2022-2024
Indiana University Health
2024
Tea Research Institute
2024
Vaccines aim to elicit a robust, yet targeted, immune response. Failure of vaccine such response arises in part from inappropriate temporal control over antigen and adjuvant presentation the system. In this work, we sought exploit system's natural extended pathogen exposure during infection by designing an easily administered slow-delivery platform. We utilized injectable self-healing polymer–nanoparticle (PNP) hydrogel platform prolong codelivery components demonstrated that these hydrogels...
Abstract The ideal vaccine against viruses such as influenza and SARS-CoV-2 must provide a robust, durable broad immune protection multiple viral variants. However, antibody responses to current vaccines often lack robust cross-reactivity. Here we describe polymeric Toll-like receptor 7 agonist nanoparticle (TLR7-NP) adjuvant, which enhances lymph node targeting, leads persistent activation of cells responses. When mixed with alum-adsorbed antigens, this TLR7-NP adjuvant elicits...
Both B cell receptor (BCR) and CD40 signaling are rewired in germinal center (GC) cells (GCBCs) to synergistically induce c-MYC phosphorylated S6 ribosomal protein (p-S6), markers of positive selection. How interleukin-21 (IL-21), a key T follicular helper (T FH )–derived cytokine, affects GCBCs is unclear. Like BCR signals, IL-21 (IL-21R) plus signals also synergize p-S6 GCBCs. However, IL-21R stimulation differentially GCBC fate compared with ligation—engaging unique molecular...
Adjuvants enhance the magnitude and durability of immune response to vaccines. However, there is a paucity comparative studies on nature responses stimulated by leading adjuvant candidates. In this study, we compared five clinically relevant adjuvants in mice-alum, AS03 (a squalene-based supplemented with α-tocopherol), AS37 TLR7 ligand emulsified alum), CpG1018 TLR9 O/W 1849101 adjuvant)-for their capacity stimulate when combined subunit vaccine under clinical development. We found that all...
Adjuvants hold great potential in enhancing vaccine efficacy, making the understanding and improving of adjuvants critical goals vaccinology. The TLR7/8 agonist, 3M-052, induces long-lived humoral immunity non-human primates is currently being evaluated human clinical trials. However, innate mechanisms 3M-052 have not been fully characterized. Here, we perform flow cytometry, single cell RNA-seq ATAC-seq to profile kinetics, transcriptomics epigenomics immune cells murine draining lymph...
mRNA vaccines have proven to be pivotal in the fight against COVID-19. A recommended booster, given 3 4 weeks post initial vaccination, can substantially amplify protective antibody levels. Here, we show that, compared contralateral boost, ipsilateral boost of SARS-CoV-2 vaccine induces more germinal center B cells (GCBCs) specific receptor binding domain (RBD) and generates bone marrow plasma cells. Ipsilateral rapidly generate high-affinity RBD-specific antibodies with improved...
In black tea beverage, the interaction between theaflavins (TFs) and proteins may lead to precipitates' formation, affecting its clarity shelf life. This study investigated interactions TF five exogenous (β-lactoglobulin (β-Lg), zein, soy protein isolate, gelatin collagen) by spectrometry measurements. The technique for order preference similarity an ideal solution (TOPSIS) was applied select optimal concentration prepare a high-TF beverage with high clarity. results showed interact...
The immune system responds preferentially to particular antigenic-epitopes contained within complex immunogens, such as proteins or microbes. This poorly understood phenomenon, termed "immunodominance," remains an obstacle achieving polyvalent responses against multiple through vaccination. We observed profound suppression in the hapten-specific antibody response mice immunized with hapten-protein conjugate, mixed excess of protein, relative that alone. was robust (100-fold and 10-fold a 10-...
Signaling through the BCR can drive B cell activation and contribute to differentiation into Ab-secreting plasma cells. The positive signal is counterbalanced by a number of membrane-localized inhibitory receptors that limit differentiation. Deficiencies in these negative signaling pathways may cause autoantibody generation autoimmune disease both animal models human patients. We have previously shown transcription factor Ets1 restrain In this study, we tested roles controlling expression...
Atopic dermatitis (AD) is the most common inflammatory skin disease with no well-delineated cause or effective cure. Here we show that p53 family member p63, specifically ΔNp63, isoform has a key role in driving keratinocyte activation AD. We find overexpression of ΔNp63 transgenic mouse epidermis results severe phenotype shares many clinical, histological and molecular features associated human This includes pruritus, epidermal hyperplasia, aberrant differentiation, enhanced expression...
CD5 is a 67-kDa glycoprotein expressed on the cell surface membrane of all T lymphocytes and small proportion B lymphocytes. The physiologic role this Ag still unknown. Structural functional studies suggest that it might act as receptor for positive signal. CD5-specific mAb augment CD3- or mitogen-induced proliferation, IL-2 secretion, IL-2R expression induce rise in intracellular [Ca2+]. In report, we describe purification mouse protein (mCD5) its use probe to search ligand CD5. We...
The Epstein-Barr virus (EBV) latent membrane protein 1 (LMP1) oncoprotein has been shown to mediate activation of the signal transducer and activator transcription 3 (STAT3). In present study, we delineated mechanism by which LMP1 stimulates STAT3 in a human nasopharyngeal carcinoma (NPC) cell line. stimulated Tyr 705-dependent nuclear accumulation, as well phosphorylation at both 705 Ser 727. Treatment cells with interleukin-6 neutralizing antibody inhibited differential was found be result...
In the 1980s, a novel tea species, Cocoa (Camellia ptilophylla Chang), was discovered in Southern China with surprisingly low caffeine content (0.2% by dry weight). Although its health promoting characteristics have been known for while, very limited amount of scientific research has focused on tea. Herein, systematic study and chemical components, interactions bioactivities performed. YD (Yunnan Daye tea, Camellia sinensis), species high (5.8% weight), used as control. By UV-Vis...