A5114585132- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Neuroscience and Neural Engineering
- 3D Printing in Biomedical Research
- Electrowetting and Microfluidic Technologies
- Nanofabrication and Lithography Techniques
- Advanced biosensing and bioanalysis techniques
- HIV/AIDS drug development and treatment
- HIV Research and Treatment
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Quantum Dots Synthesis And Properties
- Advanced Sensor and Energy Harvesting Materials
- Pneumonia and Respiratory Infections
- Solar-Powered Water Purification Methods
- Advanced Memory and Neural Computing
- Microfluidic and Bio-sensing Technologies
- Electrochemical Analysis and Applications
- Neural dynamics and brain function
- Graphene research and applications
State Key Laboratory of Transducer Technology
2013-2025
Shanghai Institute of Microsystem and Information Technology
2013-2025
Chinese Academy of Sciences
2014-2025
University of Chinese Academy of Sciences
2024
Kunming Third People's Hospital
2021
Xianning Central Hospital
2020
Hubei University of Science and Technology
2020
Forschungszentrum Jülich
2013
For a comprehensive understanding of cells or tissues, it is important to enable multiple studies under the controllable microenvironment chip. In this report, we present an integrated microfluidic cell culture platform in which endothelial (ECs) are static conditions exposed pulsatile and oscillatory shear stress. Through integration microgap, self-contained flow loop, pneumatic pumps, valves, novel chip achieved functions: fluid circulation, trapping, culture, formation ECs barrier, adding...
In a chamber-based digital PCR (dPCR) chip fabricated with polydimethylsiloxane (PDMS), bubble generation in the chambers at high temperatures is critical issue. Here, we found that main reason for formation PDMS chips too-high saturated vapor pressure of water an elevated temperature. The bubbles should be completely prevented by reducing initial system to under 13.6 kPa eliminate effects increased-pressure vapor. Then, cavity was designed and above reaction layer, Parylene C used as shell...
A portable, disposable, and low-waste sample discretization system is presented for digital assays in resource-poor settings point-of-care diagnostics.
Cerebral organoids derived from human induced pluripotent stem cells (iPSCs) have emerged as powerful in vitro models for studying brain development and neurological disorders. Understanding the electrophysiological properties of these is crucial evaluating their functional maturity potential applications. However, differentiation maturation into cerebral a long, slow, error-prone process. Hence, it vitally to establish non-invasive method monitoring process over long period time. In this...
Graphene represents a high-performance material for field effect transistor (FET) manufacture. As monolayer of carbon atoms, graphene offers flexibility, high carrier mobility, and transparency. Solution-gated FETs based on flexible substrates have been developed various applications. However, none these offer both flexibility good insulation from the electrolyte simultaneously. This would restrict their applicability in certain areas, such as implantation. To solve this problem, we used...
Digital polymerase chain reaction (dPCR) is emerging as a powerful method for nucleic acid detection due to its unprecedented sensitivity and precision. However, most current dPCR platforms are inherently limited by their low multiplexing ability primer-pair cross interactions spectral overlap of available fluorophores. Here, we present novel robust that free from primer dimerization fluorescence channel number limitation, enabling highly precise multiplexed targets. By prestoring...
Mycoplasma pneumonia (MP) is a common respiratory infection generally treated with macrolides, but resistance mutations against macrolides are often detected in mycoplasma pneumoniae China. Rapid and accurate identification of its mutant type necessary for precise medication. This paper presents 3D-printed microfluidic device to achieve this. By 3D printing, the stereoscopic structures such as microvalves, reservoirs, drainage tubes, connectors were fabricated one step. The integrated...
In this paper, the thermal performance of a heater on membrane was analyzed from ring by conventional simplified model. Some preliminary principles structure design are concluded for heaters working in vacuum or atmosphere. According to principles, an optimized is demonstrated, and coupled thermal-electrical finite element method (ANSYS) employed simulate structures. Experimental results indicated that temperature distributions quite agreement with simulations large uniform-temperature area.
We report a novel Polydimethylsiloxane (PDMS) based digital PCR (dPCR) chip with an integrated dual-function cavity that accelerates sample loading before the reaction and reduces water loss during thermal cycles. Neither external pump nor degassing setup such as vacuum chamber is needed for loading. A regular syringe sufficient delivery partition. In addition, time shortened to four minutes in reduced negligible level. Therefore, this would provide effective affordable solution on-site detection.
Surface plasmon resonance biosensor technique was used to study the binding of Moloney murine leukemia virus reverse transcriptase without RNase H domain (MMLV RT-) with DNA in absence and presence inhibitors. Different substrates, including single-stranded (ssDNA), template-primer (T-P) duplex gapped DNA, were immobilized on chip surface using streptavidin-biotin, MMLV RT(-)-DNA kinetics analyzed by different models. RT-; could bind ssDNA involved conformation change. T-P be simple 1:1...
In droplet-based microfluidics, visualizing and modulating of droplets is often prerequisite. this paper, we report a facile strategy for high-throughput in microfluidics. the strategy, by sampling frequency flash light with droplet frequency, are able to map real high signal low signal, which facilitates feedback controlling. Meanwhile, because not needing synchronization signals, can be directly implemented on any microfluidic chips. The only cost an additional generator. Moreover, catch...
We revealed the reason for bubble formation in a PDMS-based digital PCR (dPCR) chip: porous PDMS material drives water vapor out of chip at high temperature and sucks air into low temperature. Therefore, dPCR system where is enclosed with high-pressure developed. The elevated pressure to prevent direct exchange peripheral inner during thermal cycles, which turn eliminates seal valve structures from chips. Moreover, cold hot are rapidly flushed system, enabling precise, rapid, efficient...
Multi-organ chips are effective at emulating human tissue and organ functions replicating the interactions among tissues organs. An arrayed brain-heart chip was introduced whose configuration comprises open culture chambers closed biomimetic vascular channels distributed in a horizontal pattern, separated from each other by an endothelial barrier based on fibrin matrix. A 300 μm-high 13.2 mm-long surrounded organoid chamber, thereby satisfying material transport requirements. Numerical...
In this study, a high-pressure liquid environment thin-PDMS-film digital PCR (dPCR) system was developed. This platform features high quality, large surface area, low cost, fast thermal conduction speed, and easy operation, with film thickness of 400 μm dimensions 100 mm ⅹ 90 mm. Samples could be digitally processed within s. And accurate temperature shifts were achieved in designated reaction unit array area by Peltier unit. Moreover, heat-insulation lid downwardly pointed edge designed to...
This paper describes a simple method for the assembly of three-dimensional (3D) PDMS microfluidic devices using zipper-based microchannel alignment scheme. Mimicking interlocking teeth zippers, two layers are casted with interdigitated so that microchannels can be easily and rapidly realized under regular stereoscope or inverted microscope, no need any systems skillful operators. In addition, this would also tolerate small-degree mismatch between due to shrinkage when replicating structures,...
Elevated Yes-associated protein (YAP1) expression is associated with colorectal cancer. Bioinfor-matics analysis showed a targeting relationship between miR-194 and YAP13′-UTR. Our study assessed miR-194’s role in proliferation, apoptosis, CDDP resistance of cancer cells. The CDDP-resistant cell line SW480/CDDP was established YAP1 level parental SW480 cells normal intestinal epithelial HCoEpiC measured. were separated into control group, miR-NC group mimic followed by level, apoptosis...
Abstract Background Tuberculosis (TB), a multi-systemic disease with protean presentation, remains major global health problem. Although concurrent pulmonary tuberculosis (PTB) and extrapulmonary (EPTB) cases are commonly observed clinically, knowledge regarding PTB-EPTB is limited. Here large-scale multi- center observational study conducted in China aimed to epidemiology of by diagnostically defining TB types then implementing association rules analysis. Methods This was at 21 hospitals 15...
Rapid prototyping of Polydimethylsiloxane (PDMS) chips is essential in iterative design processes, but many the fabrication steps require handcrafting and haven't been adapted to automated manufacturing. This research proposes a novel method manufacturing high-precision PDMS chips, named "soft casting". A key component multifunctional removable cover that non-adhesive, gas permeable, semi-rigid. Using this method, with precise shape thickness can be manufactured. In addition, casting"...