A5075780850- Lung Cancer Research Studies
- Neuroendocrine Tumor Research Advances
- Metastasis and carcinoma case studies
- Genetic factors in colorectal cancer
- Gastric Cancer Management and Outcomes
- Colorectal Cancer Screening and Detection
- Colorectal Cancer Treatments and Studies
- Pancreatic and Hepatic Oncology Research
- Lung Cancer Treatments and Mutations
- Colorectal and Anal Carcinomas
- Gastrointestinal Tumor Research and Treatment
- Gastrointestinal disorders and treatments
- Helicobacter pylori-related gastroenterology studies
- Esophageal Cancer Research and Treatment
- Acute Myeloid Leukemia Research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Lymphoma Diagnosis and Treatment
- Acute Lymphoblastic Leukemia research
- Cell Adhesion Molecules Research
- Epigenetics and DNA Methylation
- Colorectal Cancer Surgical Treatments
- Cancer-related gene regulation
- Sarcoma Diagnosis and Treatment
- Uterine Myomas and Treatments
- Thyroid Cancer Diagnosis and Treatment
Tokyo National Hospital
2014-2025
National Cancer Center Hospital East
2018-2023
National Cancer Center
2022
National Cancer Centre Japan
2021
Experimental Pathology Laboratories
2017-2018
Juntendo University
2017
Kyoto University Hospital
2014
Nagaoka University of Technology
2012
Next-generation sequencing (NGS) of tumor tissue (ie, clinical sequencing) can guide management by providing information about actionable gene aberrations that have diagnostic and therapeutic significance. Here, we undertook a hospital-based prospective study (TOP-GEAR project, 2nd stage) to investigate the feasibility utility NGS-based analysis 114 cancer-associated genes (the NCC Oncopanel test). We examined 230 cases (comprising more than 30 types) advanced solid tumors, all which were...
The molecular mechanisms underlying the serrated pathway of colorectal tumourigenesis, particularly those related to traditional adenomas (TSAs), are still poorly understood. In this study, we analysed genetic alterations in 188 polyps, including hyperplastic sessile adenomas/polyps (SSA/Ps), TSAs, tubular adenomas, and tubulovillous by using targeted next-generation sequencing reverse transcription-PCR. Our analyses showed that most TSAs (71%) contained WNT components. particular,...
Uterine adenomyosis is a benign disorder that often co-occurs with endometriosis and/or leiomyoma, and impairs quality of life. The genomic features are unknown. Here we apply next-generation sequencing to (70 individuals 192 multi-regional samples), as well co-occurring leiomyoma endometriosis, find recurring KRAS mutations in 26/70 (37.1%) cases. Multi-regional reveals oligoclonality adenomyosis, some also detected normal endometrium endometriosis. more frequent cases low progesterone...
Abstract The neuroendocrine carcinoma of the gastrointestinal system (GIS-NEC) is a rare but highly malignant neoplasm. We analyzed 115 cases using whole-genome/exome sequencing, transcriptome DNA methylation assays, and/or ATAC-seq and found GIS-NECs to be genetically distinct from tumors (GIS-NET) in same location. Clear genomic differences were also evident between pancreatic NECs (Panc-NEC) nonpancreatic (Nonpanc-NEC). Panc-NECs could classified into two subgroups (i.e., “ductal-type”...
Objective Cancer-associated fibroblasts (CAFs), a major component of cancer stroma, can confer aggressive properties to cells by secreting multiple factors. Their phenotypes are stably maintained, but the mechanisms not fully understood. We aimed show critical role epigenetic changes in CAFs maintaining their tumour-promoting capacity and validity epigenomic approach identifying therapeutic targets from starve cells. Design Twelve pairs primary gastric corresponding non-CAFs (NCAFs) were...
425 Background: Definitive chemoradiotherapy (dCRT) is the standard treatment for unresectable locally advanced esophageal squamous cell carcinoma (LA-ESCC) based on results of JCOG 0303 study. However, prognosis in this population remains poor with a median overall survival (OS) 13.1 months, and fistula formation was reported about 20% patients who received dCRT. In such circumstance, more effective safer strategy, as induction chemotherapy followed by conversion surgery or dCRT, developed....
Sessile serrated adenoma/polyps (SSA/Ps) are believed to be the major precursor of pathway-derived colorectal carcinomas. To better characterize process progression from SSA/Ps carcinomas, we analyzed 46 with dysplasia and 45 without using targeted next-generation sequencing immunohistochemistry. Among WNT pathway genes analyzed, protein-truncating mutations RNF43, APC, ZNRF3 were identified in 23 (50%), 4 (9%), 3 (7%) dysplasia, respectively. In contrast, rarely had gene mutations, except...
Aims Familial adenomatous polyposis ( FAP ) is a hereditary cancer predisposition syndrome caused by germline APC mutation. A recent study showed the enrichment of pyloric gland adenomas PGA s) stomach, in addition to fundic polyps FGP and foveolar‐type FA s), patients with . In present study, we analysed genetic alterations these ‐associated gastric lesions. Methods results Mutational statuses GNAS KRAS , which are frequently mutated sporadic s, as well those were examined s using Sanger...
The presence of intestinal metaplasia (IM) is a risk factor for gastric cancer. However, it still controversial whether IM itself precancerous or paracancerous. Here, we aimed to explore the nature by analysing epigenetic alterations.
Aims Traditional serrated adenoma ( TSA ) is a rare but distinct type of colorectal polyp. Our previous study showed that PTPRK – RSPO 3 fusions are frequent and characteristic genetic alterations in s. This aimed to characterize comprehensively the prevalence variability Methods results We examined expression explored novel 129 s, including 66 lesions analysed previously for WNT pathway gene mutations. Quantitative polymerase chain reaction qPCR analyses identified three 43 s overexpressing...
Abstract Helicobacter pylori (HP) drives a chronic inflammation in the gastric mucosa, leading to atrophic gastritis, intestinal metaplasia (IM), and further culminating cancer. Oncogenesis by HP infection has been mainly attributed epigenetic dysregulation, recent advances understanding of clonal expansion have shed light on mutational architecture IM. However, these analyses performed separately, leaving our fragmented. Furthermore, small-sized tissue cannot determine spatial events entire...
ABSTRACT Adenosquamous carcinoma is an uncommon type of cancer that comprises malignant squamous and glandular components. We present a case human papillomavirus (HPV)‐positive adenosquamous the anorectum, which exhibited extensive pagetoid spread, in 70‐year‐old woman. The tumor had spread from lower rectum to perianal skin was removed through combined endoscopic transanal surgical procedure. Histological examination revealed three morphologically distinct components: adenocarcinoma rectum,...
We describe a series of colorectal polyps characterized by mixed adenomatous and serrated features, herein referred to as superficially adenomas. Twenty adenomas were obtained from 11 female 9 male patients aged 62-87 years. Most lesions endoscopically appeared small sessile polyps, but larger plaque-like (2-20 mm; median, 5 mm). Eighteen (90%) located in the sigmoid colon or rectum. They consisted primarily straight, glands showed serration confined superficial layer. Immunohistochemistry...
Aims Traditional serrated adenoma (TSA) is an uncommon type of colorectal polyp. RSPO fusions, which potentiate WNT signalling, are common and characteristic genetic alterations in TSA. The aim this study was to further characterise the prevalence variation fusions Methods results Quantitative polymerase chain reaction (PCR) analysis 99 TSAs revealed overexpression RSPO2 RSPO3 six 29 lesions, respectively. Reverse transcription PCR identified previously reported PTPRK – fusion transcripts...
Colorectal traditional serrated adenomas (TSAs) are often associated with precursor polyps, including hyperplastic polyps and sessile adenoma/polyps. To elucidate the molecular mechanisms involved in progression from to TSAs, present study analyzed 15 polyp-associated TSAs harboring WNT pathway gene mutations. Laser microdissection-based sequencing analysis showed that BRAF or KRAS mutations were shared between TSA all lesions. In contrast, statuses of different 2 components. 8 lesions,...
Abstract Anal squamous cell carcinoma (ASCC) is a rare tumor of the gastrointestinal tract. We aimed to compare genetic backgrounds and their effect on clinical outcomes between Japanese Caucasian patients with ASCC. Forty-one diagnosed ASCC at National Cancer Center Hospital were enrolled evaluated for clinicopathological features, human papillomavirus (HPV) infection, HPV genotypes, p16 expression, PD-L1, association status efficacy concurrent chemoradiotherapy (CCRT). Target sequencing...
Gastric foveolar-type adenoma (FA) is a rare benign neoplasm occurring either sporadically or in patients with familial adenomatous polyposis (FAP). However, the molecular features of FA and relationship between sporadic syndromic lesions remain unclear. In this study, we performed clinicopathological, immunohistochemical, genetic analyses 18 30 FAP-associated FAs. Most FAs were located upper middle third stomach, on background fundic gland mucosa. low-grade, but 3 had high-grade component....
Abstract Background The standard preoperative treatment for resectable locally advanced esophageal squamous cell carcinoma (LAESCC) in Japan is docetaxel, cisplatin (CDDP), and 5-fluorouracil. However, patients with renal or cardiac dysfunction elderly are ineligible a CDDP-containing regimen because of toxicities. Oxaliplatin, leucovorin, 5-fluorouracil (FOLFOX) therapy has less toxicity than regimens does not require hydration. there limited data on FOLFOX these patients. Methods This...