A5065401280- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- SARS-CoV-2 and COVID-19 Research
- T-cell and B-cell Immunology
- CAR-T cell therapy research
- Ultrasound and Hyperthermia Applications
- COVID-19 Clinical Research Studies
- Photoacoustic and Ultrasonic Imaging
- Monoclonal and Polyclonal Antibodies Research
- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Nanoplatforms for cancer theranostics
- Inflammatory Biomarkers in Disease Prognosis
- Immune cells in cancer
- Nanoparticle-Based Drug Delivery
- Cytomegalovirus and herpesvirus research
- Pancreatic function and diabetes
- Ultrasound and Cavitation Phenomena
- Hepatitis B Virus Studies
- Influenza Virus Research Studies
- Histone Deacetylase Inhibitors Research
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Phagocytosis and Immune Regulation
TagCyx Biotechnologies (Japan)
2023
National Institute of Infectious Diseases
2021
New Tokyo Hospital
2021
Tohoku University
2009-2018
Japan Science and Technology Agency
2015-2016
Two doses of Pfizer/BioNTech BNT162b2 mRNA vaccine elicit robust severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-neutralizing antibodies with frequent adverse events. Here, by applying a high-dimensional immune profiling on 92 vaccinees, we identify six vaccine-induced dynamics that correlate the amounts neutralizing antibodies, severity events, or both. The early natural killer (NK)/monocyte subsets (CD16+ NK cells, CD56high and non-classical monocytes), dendritic cell (DC)...
A still unanswered question is what drives the small fraction of activated germinal center (GC) B cells to become long-lived quiescent memory cells. We found here that a population GC-derived CD38intBcl6hi/intEfnb1+ with lower mTORC1 activity favored cell fate. Constitutively high led defects in formation cells; conversely, decreasing resulted relative enrichment this memory-prone over recycling-prone one. Furthermore, had higher levels Bcl2 and surface BCR that, turn, contributed their...
Immune checkpoint inhibitors (ICIs) have caused revolutionary changes in cancer treatment, but low response rates remain a challenge. Semaphorin 4A (Sema4A) modulates the immune system through multiple mechanisms mice, although role of human Sema4A tumor microenvironment remains unclear. This study demonstrates that histologically Sema4A-positive non–small cell lung (NSCLC) responded significantly better to anti–programmed death 1 (PD-1) antibody than Sema4A-negative NSCLC. Intriguingly,...
Adaptive immunity is a fundamental component in controlling COVID-19. In this process, follicular helper T (Tfh) cells are subset of CD4+ that mediate the production protective antibodies; however, SARS-CoV-2 epitopes activating Tfh not well characterized. Here, we identified and crystallized TCRs public circulating (cTfh) clonotypes expanded patients who have recovered from mild symptoms. These recognized spike (S) conserved across emerging variants. The epitope most prevalent cTfh...
Sonoporation is achieved by ultrasound-mediated destruction of ultrasound contrast agents (UCA) microbubbles. For this, UCAs must be tissue specific and have good echogenicity also function as drug carriers. Previous studies developed acoustic liposomes (ALs), that encapsulate phosphate buffer solution perfluoropropane (C3F8) gas both Few examined the co-existence liquid in ALs. This study aims to elucidate AL structure using TEM. The size, zeta potential ALs were compared with those two...
The transcription factor Bach2 regulates both acquired and innate immunity at multiple steps, including antibody class switching regulatory T cell development in activated B cells, respectively. However, little is known about the molecular mechanisms of regulation response to signaling cytokines antigen. We show here that mammalian target rapamycin (mTOR) controls along differentiation with two distinct pre-B cells. First, mTOR complex 1 (mTORC1) inhibited accumulation protein nuclei reduced...
Broadly protective vaccines against SARS-related coronaviruses that may cause future outbreaks are urgently needed. The SARS-CoV-2 spike receptor-binding domain (RBD) comprises two regions, the core-RBD and motif (RBM); former is structurally conserved between SARS-CoV. Here, in order to elicit humoral responses more core-RBD, we introduced N-linked glycans onto RBM surfaces of RBD used them as immunogens a mouse model. We found glycan addition elicited higher proportions core-RBD-specific...
Abstract BTB and CNC homology 2 (Bach2) is a transcriptional repressor that required for the formation of germinal center (GC) reactions, including class switch recombination somatic hypermutation Ig genes in B cells, within GC. Although BCR-induced proliferation essential GC function Bach2 regulating cell has not been elucidated. In this study, we demonstrate to sustain high levels response BCR signaling. Following engagement vitro, cells from Bach2-deficient (Bach2−/−) mice showed lower...
Severe acute respiratory syndrome coronavirus 2-neutralizing antibodies primarily target the spike receptor binding domain (RBD). However, B cell antigen receptors (BCRs) on RBD-binding memory (Bmem) cells have variation in neutralizing activities. Here, by combining single Bmem profiling with antibody functional assessment, we dissected phenotype of harboring potently disease 2019 (COVID-19)-convalescent individuals. The subset was marked an elevated CD62L expression and characterized...
Some patients experience mixed response to immunotherapy, whose biological mechanisms and clinical impact have been obscure. We obtained two tumor samples from lymph node (LN) metastatic lesions in a same patient. Whole exome sequencing for the both tumors single-cell tumor-infiltrating lymphocytes (TIL) demonstrated significant difference clonality TILs' characteristics, especially exhausted T-cell clonotypes, although close relationship between cell clones were observed as of an overlapped...
Recent basic and clinical studies have assessed the use of highly sensitive imaging modalities for visualizing transplanted islets. We investigated utility enhanced ultrasonography, combined with fluorescent acoustic liposome nano/microbubbles (FALs), evaluating angiogenesis endocrine function BALB/c mice were classified into three groups: Diabetic that underwent syngeneic islet transplantation subrenal capsule achieved normoglycemia (Tx group); those failed to achieve (Tx-DM not receiving...
Two doses of Pfizer/BioNTec BNT162b2 mRNA vaccine elicit robust SARS-CoV-2-neutralizing antibodies with frequent adverse events. Here, by applying a high-dimensional immune profiling on 92 vaccinees, we identified six vaccine-induced dynamics that correlated the amounts neutralizing antibodies, severity events, or both. The early natural killer/monocyte subsets and two dendritic cell were revealed as distinct correlates for antibody titers respectively. Indeed, combination classified...
We attempted to clarify the usefulness of enhanced ultrasonography (US) using fluorescent acoustic liposomes (FALs) for detecting transplanted islets. FALs consisted 4 types phospholipid with dye. Diabetic BALB/c mice, which were performed syngeneic islet transplantation into subrenal capsule and achieved normoglycemia (Tx group), failed in (Tx-DM group) did not received any treatments (DM done FALs-enhanced high frequency US (HF-US) examinations at postoperative day (POD) 14, 28 56....
<div><p>Some patients experience mixed response to immunotherapy, whose biological mechanisms and clinical impact have been obscure. We obtained two tumor samples from lymph node (LN) metastatic lesions in a same patient. Whole exome sequencing for the both tumors single-cell tumor-infiltrating lymphocytes (TIL) demonstrated significant difference clonality TILs’ characteristics, especially exhausted T-cell clonotypes, although close relationship between cell clones were observed...
<div><p>Some patients experience mixed response to immunotherapy, whose biological mechanisms and clinical impact have been obscure. We obtained two tumor samples from lymph node (LN) metastatic lesions in a same patient. Whole exome sequencing for the both tumors single-cell tumor-infiltrating lymphocytes (TIL) demonstrated significant difference clonality TILs’ characteristics, especially exhausted T-cell clonotypes, although close relationship between cell clones were observed...